Sodium chloride and nitrogen effects on Chlorella vulgaris growth and biocommodities production

Biliani, Styliani E.; Manariotis, Ioannis D.

doi:10.5004/dwt.2021.27729

Cited by 4 publications

(2 citation statements)

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“…An increase in cell size was also recorded by other specialists, for example, when using scanning electron microscopy, an increase in cell size by 33.52% (in Chlorella humicola) and 27.79% (in Chlorella vulgaris) was noted at 100 mm sodium chloride [35]. This may be due to the fact that sodium chloride leads to an increase in lipids, proteins, carbohydrates and thickening of the cell wall [31,36]. The results of the use of urotropin (see table.…”

Section: Resultssupporting

confidence: 53%

Cytotoxicity of some preservatives for cultureChlorella VulgarisGKO strain

Azhikina,

Makhnin,

Berenev

et al. 2023

BIO Web Conf.

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One of the ways to preserve the culture of microalgae for a long time is conservation. All modern methods of preservation and long-term storage of microorganism cultures are based on the transfer of cells to a state of anabiosis, in which metabolic processes are either completely or partially stopped. One of the mechanisms that are realized during the transition to the anabiotic state is a change in cell permeability. This can be achieved by modifying the culture medium. Thus, the search for preservatives that contribute to the long-term preservation of biomass is very relevant. The primary condition for the use of any preservative or a new component of the culture medium should be the absence of a toxic effect on the cell culture. Goal of the work: to determine the cytotoxic effect of some preservatives for the cell culture of Chlorella vulgaris GKO strain. The cytotoxicity of ascorbic acid, lactic acid, citric acid, acetic acid, sodium chloride and urotropin was analyzed for the cell culture of Chlorella vulgaris strain GKO. The following parameters were determined: pH of Chlorella vulgaris suspension with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm pH of suspension of Chlorella vulgaris with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm pH of suspension of Chlorella vulgaris with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm

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Section: Resultssupporting

confidence: 53%

Cytotoxicity of some preservatives for cultureChlorella VulgarisGKO strain

Azhikina,

Makhnin,

Berenev

et al. 2023

BIO Web Conf.

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“…Their fast growth combined with the ability to be cultivated in a variety of environments (pH, temperature, salinity) in different mediums (freshwater, saline water and wastewater) make microalgae ideal candidates for the production of biomass, lipids and biocompounds (proteins and carbohydrates) and for wastewater remediation. 2,3 Lipids can be converted into renewable biodiesel, 4,5 while proteins and carbohydrates can be used as fertilizers or in aquaculture. 6,7 Microalgae can be cultivated in suspended and attached cultures.…”

Section: Introductionmentioning

confidence: 99%

Chlorococcum sp. biofilm growth on biochar from olive kernels solid support

Tsavatopoulou

Vakros

Manariotis

2023

J of Chemical Tech & Biotech

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BACKGROUND: Biofilm is a complex community of microorganisms including microalgae, bacteria and protozoa, which are adhered to a surface. The purpose of an integrated microalgal processing system is the production of biofuels, biomass and other valuable bioproducts as well as wastewater remediation. Biochar is a carbon-rich and porous substrate, capable of adsorbing nutrients.RESULTS: In this study, biochar produced from olive kernels pyrolyzed at 400 °C was tested as a solid support candidate for adhering Chlorococcum sp. cultures. The substrate used was BG-11 with 1/3 nitrates and BG-11 without nitrates and phosphates. After 15 days of cultivation, the biomass attached to biofilm was measured, while different parameters were determined in the liquid. Among the different concentrations of biochar used, the culture containing 1 g L −1 biochar gave the best performance, resulting in 1.28 and 1.24 g microalgae g −1 biochar in the presence and absence of nitrates, respectively. CONCLUSION: The cultures containing BG-11 without nitrogen and phosphorus showed leaching of nutrients in the liquid of the reactor. The present findings provide an alternative use of biochar, enhancing the growth of microalgae, and thus encouraging its use as supporting material in bioreactors.

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Wastewater treatment by high density algal flocs for nutrient removal and biomass production

Biliani

Manariotis

2023

J Appl Phycol

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Sodium chloride and nitrogen effects on Chlorella vulgaris growth and biocommodities production

Cited by 4 publications

References 0 publications

Cytotoxicity of some preservatives for cultureChlorella VulgarisGKO strain

Cytotoxicity of some preservatives for cultureChlorella VulgarisGKO strain

Chlorococcum sp. biofilm growth on biochar from olive kernels solid support

Wastewater treatment by high density algal flocs for nutrient removal and biomass production

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