A supplemental table of sodium chloride equivalents and freezing-point depressions at various concentration for 29 substances in aqueous solution is presented. Also listed is the isosmotic concentration of each material that can form such a solution. The degree of hemolysis of human erythrocytes was determined in 12 different isosmotic solutions, and the data are presented to supplement the previously published values. While four isosmotic solutions prevented hemolysis, eight other did not.