Sodium chloride transport across the chicken coprodeum. Basic characteristics and dependence on sodium chloride intake

Abstract

SUMMARY1. The transport characteristics of the chicken coprodeum have been examined in vitro using the isolated mucosa. The short-circuit current (I,), the transepithelial electrical potential difference (p.d.), the unidirectional transmural fluxes (J., J.) of sodium and chloride measured in the short-circuited state, and the unidirectional influx of sodium and chloride across the brush border membrane measured under open-circuit conditions have been studied. The effect of the sodium chloride contents of the diet on these parameters have been investigated.2. The isolated mucosa depends functionally on the presence of glucose in the incubation media. This dependence reflects the need of glucose as a fuel. There is no indication of coupling between transport of -sugars and sodium across the brush border membrane. For preparations from chickens on a low sodium diet a very high and stable ISc can quantitatively be accounted for by the net transport of sodium. Influx of sodium across the brush border membrane is not significantly different from the net flux of sodium. By feeding the chickens a high sodium diet the I,, is reduced by more than 95 %, the net transport of sodium is abolished, and the transepithelial electrical conductance is reduced by more than 50 %.3. Both unidirectional transepithelial fluxes of chloride, and the serosa to mucosa flux of sodium appear to proceed through a paracellular shunt.4. Under the conditions of the low sodium diet the paracellular pathway appears to be anion selective. Whereas, under the conditions of the high sodium regimen the paracellular route appears to be cation selective. After adaptation to a high sodium diet the influx of sodium across the brush border membrane is only moderately reduced. Consequently the decisive event in the adaptation must be localized elsewhere.

Function of the Lower Intestine and Osmoregulation in the Ostrich: Preliminary Anatomical and Physiological Observations

“…recorded. This method (Edmonds & Godfrey, 1970) has in the hen coprodeum on a low-NaCl diet given p.d.s close to the 40-50 mV, lumen negative, recorded in Ussing-chamber experiments in vitro (Choshniak, Munck & Skadhauge, 1977).…”

“…in the chicks on a low-NaCl diet only means that the presence of an electrogenic Na transport was not demonstrated. Such transport was demonstrated in other birds such as the domestic fowl (Choshniak et al 1977) and the galah . Although other transport mechanisms are possible, their total effect on ureteral urine might be limited.…”

Luminal morphology of the avian lower intestine: Evidence supporting the importance of retrograde peristalsis for water conservation

“…Experiments with sparrows show that significant urine modification occurs in hydrated but not in dehydrated sparrows (Goldstein and Braun, 1988). In hens, salt-loading experiments show that on high salt diets the coprodeal epithelium absorbs virtually no salt, but on a lower salt diet, sodium absorption increases dramatically up to 100-fold (Chosniak et al 1977; Thomas and Skadhauge, 1982b).…”

Conversion of sodium channels to a form sensitive to cyclic AMP by component(s) from red cells

“…The avian coprodaeum behaves rather as the rat in that amiloride-sensitive sodium transport appears only after salt deprivation (Choshniak, Munck & Skadhauge, 1977;Bindslev, Cuthbert, Edwardson & Skadhauge, 1982).…”