Sodium Cromoglycate Prevents Exacerbation of IgE-Mediated Food-Allergic Reaction Induced by Aspirin in a Rat Model of Egg Allergy

Abstract

Background: Aspirin (ASP)-facilitated absorption of ingested allergens is considered an exacerbating factor in the development of food allergy. Sodium cromoglycate (SCG) is used for the treatment of atopic dermatitis with food allergy, but the efficacy of SCG in ASP-exacerbated food-allergy reactions is unclear. In this study, we evaluated the effect of SCG on ASP-exacerbated food-allergic reactions, as well as allergen absorption, in egg-allergic model rats. Methods: Plasma concentrations of ovalbumin (OVA) and fluorescein isothiocyanate-labeled dextran (FD-40), a marker for nonspecific-absorption pathways, were measured after oral administration of mixtures of OVA and FD-40 in OVA-unsensitized and OVA-sensitized rats. IgE-mediated allergic reactions were evaluated by measuring changes in rectal temperature and Evans blue dye (EBD) extravasation in the intestine and liver after oral challenge with OVA. The effects of ASP and SCG on such absorption and allergic reactions were also evaluated kinetically. Results: In OVA-sensitized rats, plasma concentrations of OVA and FD-40 were significantly higher than those in unsensitized rats after oral administration. ASP increased the intestinal absorption of OVA and FD-40 via the paracellular pathway, and a lower rectal temperature and higher EBD extravasation were detected in the intestine and liver of OVA-sensitized rats. SCG ameliorated these ASP-facilitated absorptions and allergic reactions in a dose-dependent manner. In particular, high-dose SCG (195.2 μmol/kg) completely inhibited these absorptions and reactions. Conclusion: SCG can prevent ASP-exacerbated allergic reactions in patients with food allergy resulting from inhibition of increases in allergen absorption.

Aspirin enhances sensitization to the egg-white allergen ovalbumin in rats

“…Previous reports have shown that aspirin induced and exacerbated allergic symptoms by promoting absorption of ingested allergens in rats and patients with IgE-mediated food allergies [7–9,11]. However, there are no reports regarding the effect of aspirin on the sensitization to ingested allergens.…”

“…We previously demonstrated that OVA were absorbed from intestine as an intact form in the presence of protease inhibitors using in situ intestinal perfusion technique [5]. In addition, aspirin elicited and/or exacerbated the allergic symptoms by increasing oral absorptions of OVA [9]. These reports suggested that aspirin could enhance the oral absorption of OVA as an intact form at least partly.…”

“…To evaluate the effects of aspirin on the absorption of an ingested allergen, plasma levels of OVA in rats were examined as reported previously [9]. Briefly, after overnight fasting, rats were anesthetized with pentobarbital (30 mg/kg, i .…”

“…Each blood sample was centrifuged, and the plasma sample was stored at −30°C until use. The plasma concentration of OVA was determined using a sandwich enzyme-linked immunosorbent assay (ELISA) kit (Morinaga Institute of Biological Science, Yokohama, Japan) and a Microplate Fluorometer (PerkinElmer, Waltham, MA, USA) at a wavelength of 500 nm for excitation and 520 nm for emission, as described previously [9].…”

Evaluation of the allergenicity of ω5-gliadin-deficient Hokushin wheat (1BS-18) in a wheat allergy rat model

“…In this study, OVA, ω5-gliadin, Hokushin gluten or 1BS-18 gluten were intravenously administered because we can evaluate their allergenicities while excluding processes of digestion and absorption. Rectal temperature was measured using a specific rectal thermometer for rats (Shibaura Electronics, Saitama, Japan) before and 30 min after intravenous challenge according to a previous report [16]. After measuring the rectal temperature at 0 min, OVA, ω5-gliadin, Hokushin gluten or 1BS-18 gluten (10 mg/kg) were intravenously injected via a canula inserted in the femoral vein.…”