Sodium current inhibition by nanosecond pulsed electric field (nsPEF)—fact or artifact?

Verkerk, Arie O.; Ginneken, A. C. G. Van; Wilders, Ronald

doi:10.1002/bem.21754

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…However, neither tetrodotoxin nor ranolazine produced any effects on PALCAR-stimulated I MEP found in these cells. Taken together, results from these observations lead us to propose that, in GH 3 cells, PALCAR has a stimulatory effect on I MEP , rather than on late Na + currents [21,28,29. ]…”

Section: Resultsmentioning

confidence: 99%

Identification of Minuscule Inward Currents as Precursors to Membrane Electroporation-Induced Currents: Real-Time Prediction of Pore Appearance

Tsai

et al. 2013

Cell Physiol Biochem

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Background/Aims: The objective of this study is to examine the current signals in response to large hyperpolarizations with the aid of principal component analysis (PCA) to search for or even predict current fluctuations related to membrane electroporation-induced current (I_MEP). Methods: The characteristics of principal eigenvalues generated for I_MEP and the current signals at 10 sec prior to the start of initial I_MEP (I_Pre) were examined. As membrane hyperpolarizations were applied at 0.1 Hz, the appearance of I_MEP coincided with the higher principal eigenvalues extracted in PCA. Results: Subsequent addition of LaCl₃ (100 µM) greatly reduced I_MEP and associated principal eigenvalues. In real-time analysis for a single frame (i.e, 300 msec), in response to large hyperpolarization, multiple runs of heralded minuscule inward currents (I_min) occurring before large rise in current amplitudes were detected. With PCA, such heralded I_min was noted to coincide with the extreme principal eigenvalues. The duration of I_min together with large principal eigenvalues was influenced by different levels of membrane hyperpolarization. In GH3 cells, palmitoyl-L-carnitine (PALCAR), a long-chain acylcarnitine, effectively increased the I_MEP amplitude with an EC₅₀ value of 2.4 µM. However, in PALCAR-treated cells, the I_min together with higher principal eigenvalues disappeared, while in isoflurane-treated cells, I_min occurring before large rise of current amplitude remained intact. Similarly, the PCA analysis from I_Pre in RAW 264.6 macrophages showed the presence of herald I_min accompanied by the extreme principal eigenvalues. Conclusion: It is clear from this study that these large principal eigenvalues are representative of MEP-associated formation of electropores. Therefore, different compositions around the surface membrane of cells may alter the appearance of I_min followed by I_MEP emergence.

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Section: Resultsmentioning

confidence: 99%

Identification of Minuscule Inward Currents as Precursors to Membrane Electroporation-Induced Currents: Real-Time Prediction of Pore Appearance

Tsai

et al. 2013

Cell Physiol Biochem

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“…Combined total impact of different external magnetic fields on the genesis of biological objects makes it difficult to determine the role of each contributing field separately and, consequently, to identify plausible mechanisms of biomagnetic effects. Most current studies deal with the impact on biological objects of alternating electromagnetic fields in the natural superposition with static magnetic fields [Zhadin, ; Kim et al, ; Samsonov and Popov, ; Verkerk et al, ].…”

Section: Introductionmentioning

confidence: 99%

Effect of weak static magnetic fields on the development of cultured skeletal muscle cells

Surma

Belostotskaya

Щеголев

et al. 2014

Bioelectromagnetics

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We studied the effect produced on the development and functional activity of skeletal muscle cells from newborn Wistar rats in primary culture by weak static magnetic fields (WSMF; 60-400 µT) with a high capacity of penetrating the biological media. To reduce the impact of external magnetic fields, cells were cultured at 37 °C in a multilayered shielding chamber with the attenuation coefficient equal to 160. WSMF inside the chamber was created by a circular permanent magnet. We found that the application of WSMF with the magnetic field strength only a few times that of the geomagnetic field can accelerate the development of skeletal muscle cells, resulting in the formation of multinuclear hypertrophied myotubes. WSMF was shown to induce 1.5- to 3.5-fold rise in the concentration of intracellular calcium [Ca(2+)]i due to the release of Ca(2+) from the sarcoplasmic reticulum (SR) through ryanodine receptors (RyR), which increases in the maturation of myotubes. We also found that fully differentiated myotubes at late stages of development were less sensitive to WSMF, manifesting a gradual decrease in the frequency of contractions. However, myotubes at the stage when electromechanical coupling was forming dramatically reduced the frequency of contractions during the first minutes of their exposure to WSMF.

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“…Patch-clamp provides a precise approach to measure single or multiple ion channel responses to nanosecond pulsed electric field treatment. Some works carrying on demonstrate that nanosecond pulsed electric field may have the effect of not only long-term permeabilization but also inhibition of voltage-gated I Na and I Ca [54, 55]. …”

Section: Part I: Electrode Invented For Experiments In Vitromentioning

confidence: 99%

The Electrode Modality Development in Pulsed Electric Field Treatment Facilitates Biocellular Mechanism Study and Improves Cancer Ablation Efficacy

Cen

Chen

2017

Journal of Healthcare Engineering

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Pulsed electric field treatment is now widely used in diverse biological and medical applications: gene delivery, electrochemotherapy, and cancer therapy. This minimally invasive technique has several advantages over traditional ablation techniques, such as nonthermal elimination and blood vessel spare effect. Different electrodes are subsequently developed for a specific treatment purpose. Here, we provide a systematic review of electrode modality development in pulsed electric field treatment. For electrodes invented for experiment in vitro, sheet electrode and electrode cuvette, electrodes with high-speed fluorescence imaging system, electrodes with patch-clamp, and electrodes with confocal laser scanning microscopy are introduced. For electrodes invented for experiment in vivo, monopolar electrodes, five-needle array electrodes, single-needle bipolar electrode, parallel plate electrodes, and suction electrode are introduced. The pulsed electric field provides a promising treatment for cancer.

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Sodium current inhibition by nanosecond pulsed electric field (nsPEF)—fact or artifact?

Cited by 4 publications

References 11 publications

Identification of Minuscule Inward Currents as Precursors to Membrane Electroporation-Induced Currents: Real-Time Prediction of Pore Appearance

Identification of Minuscule Inward Currents as Precursors to Membrane Electroporation-Induced Currents: Real-Time Prediction of Pore Appearance

Effect of weak static magnetic fields on the development of cultured skeletal muscle cells

The Electrode Modality Development in Pulsed Electric Field Treatment Facilitates Biocellular Mechanism Study and Improves Cancer Ablation Efficacy

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