Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway

Velmurugan, Bharath Kumar; Lin, Shu-Hui; Mahalakshmi, B.; Lo, Yu‐Sheng; Lin, Chia-Chieh; Chuang, Yi‐Ching; Hsieh, Ming‐Ju; Chen, Mu‐Kuan

doi:10.3389/fendo.2020.568436

Cited by 20 publications

(22 citation statements)

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“…Salvianic acid A (Danshensu), a major water‐soluble component extracted from Salvia miltiorrhiza , is often converted into Sodium Danshensu to improve its chemical stability (Meng et al, 2019). A recent study showed that Sodium Danshensu inhibits oral cancer cell proliferation and metastasis (Kumar et al, 2020). However, as far as we know, whether Sodium Danshensu plays an anti‐cancer role in lung cancer remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Human normal bronchial epithelial cell (BEAS‐2B) and lung cancer lines (A549; NCI‐H1299) were obtained from ATCC (Manassas, VA, USA) and cultured in RPMI‐1640 medium (Gibco, CA, USA) supplemented with 10% FBS (Gibco, CA, USA), 1% streptomycin and penicillin in a humidified incubator with 5% CO 2 at 37°C. Then BEAS‐2B cells, A549 cells, and NCI‐H1299 cells were induced by various concentrations of Sodium Danshensu (25, 50, and 100 μM) for 24, 48, and 72 h (Kumar et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…Danshensu is the main water‐soluble component extracted from the root of Salvia miltiorrhiza , which can be converted into Sodium Danshensu to improve its chemical stability without interfering with its biological activity (Meng et al, 2019). A recent study has revealed that Sodium Danshensu could inhibit the proliferation and metastasis of oral cancer cells (Kumar et al, 2020). At present, whether Sodium Danshensu also has anti‐cancer effect in lung cancer is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Sodium Danshensu inhibits the progression of lung cancer by regulating PI3K/Akt signaling pathway

Wang

Tang

Gong

et al. 2021

Drug Development Research

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Sodium Danshensu, extracted from the root of the Salvia miltiorrhiza, has pleiotropic effects including anti‐oxidation, anti‐inflammation and anti‐tumor. However, whether Sodium Danshensu has an anti‐cancer effect in lung cancer remains to be elucidated. The present study aimed to illustrate the effects of Sodium Danshensu on lung cancer cells and the potential molecular mechanisms. BEAS‐2B, A549, and NCI‐H1299 cells were stimulated with 25, 50, and 100 μM Sodium Danshensu for 24, 48, and 72 h, and then cell viability, apoptosis, migration and invasion were determined using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), flow cytometry and Transwell assays, respectively. Moreover, the levels of Proliferating cell nuclear antigen (PCNA), matrix metalloproteinase 9 (MMP9), B‐cell lymphoma‐2 (Bcl‐2) associated X (Bax), Bcl‐2, phosphorylated (p)‐phosphoinositide 3‐kinase (PI3K), and p‐Protein kinase B (AKT) in lung cancer cells were evaluated using quantitative real‐time polymerase chain reaction (qRT‐PCR) and/or Western blot assays. We observed that Sodium Danshensu suppressed cells viability, migration, and invasion, as well as promoted cells apoptosis in A549 and NCI‐H1299 cells in a dose‐dependent manner, while Sodium Danshensu had no cytotoxic effect on the proliferation activity of BEAS‐2B cells. Moreover, the expression of PCNA, MMP9, Bcl‐2 were decreased, but Bax was up‐regulated in Sodium Danshensu‐treated A549 and NCI‐H1299 cells. Our findings also revealed that Sodium Danshensu inhibited PI3K/AKT pathway in A549 and NCI‐H1299 cells. In conclusion, our study provided the first evidence that Sodium Danshensu suppressed the malignant biological behaviors of lung cancer cells, indicating that Sodium Danshensu might be a latent candidate for lung cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sodium Danshensu inhibits the progression of lung cancer by regulating PI3K/Akt signaling pathway

Wang

Tang

Gong

et al. 2021

Drug Development Research

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“…Previous studies deciphering the anti-metastatic effects of phytochemicals have shown sodium danshensu significantly inhibits the migration and invasion of human oral cancer cells by downregulating the phosphorylation of p38 [ 21 ]. Similarly, other bioactive plant compounds, such as Kahweol acetate and desacetylnimbinene, have been shown to inhibit cancer metastasis by reducing the phosphorylation of p38 [ 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition

Velmurugan

Lin

Mahalakshmi³

et al. 2021

IJMS

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Advanced-stage oral cancers with lymph node metastasis are associated with poor prognosis and a high mortality rate. Although recent advancement in cancer treatment has effectively improved the oral cancer prognosis, the majority of therapeutic interventions are highly expensive and are associated with severe sideeffects. In the present study, we studied the efficacy of a diarylheptanoid derivative, platyphyllenone, in modulating the metastatic potential of human oral cancer cells. Specifically, we treated the human oral cancer cells (FaDu, Ca9-22, and HSC3) with different concentrations of platyphyllenone and measured the cell proliferation, migration, and invasion. The study findings revealed that platyphyllenonesignificantly inhibited the motility, migration, and invasion of human oral cancer cells. Mechanistically, platyphyllenone reduced p38 phosphorylation, decreased β-catenin and Slug, increased E-cadherin expression, and reduced cathepsin L expression, which collectively led to a reduction in cancer cell migration and invasion. Taken together, our study indicates that platyphyllenone exerts significant anti-metastatic effects on oral cancer cells by modulating cathepsin L expression, the MAPK signaling pathway, and the epithelial–mesenchymal transition process.

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“…[ 22 ] With regard to commonly used traditional Chinese medicine (TCM) herbal remedies, Danshen ( Radix Salviae Miltiorrhizae ) has reportedly demonstrated clear anti-inflammatory properties through tumor cell proliferation reduction via apoptotic cell activity and MAPK signaling decreases of oral cancer cells. [ 23 , 24 ] A meta-analysis conducted by Bae et al, [ 25 ] identified the herbal medicine exhibits the potential to increase natural killer cells in cancer patients. Furthermore, herbal medicine intervention demonstrates the potential to reduce multiple chemotherapy and radiotherapy induced side effects, such as xerostomia, gastrointestinal reactions, peripheral neuropathy, and pneumonitis.…”

Section: Introductionmentioning

confidence: 99%

Traditional Chinese medicine use in patients with oral cancer: A retrospective longitudinal cohort study in Taiwan

et al. 2022

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Oral cancer is frequently associated with smoking, alcohol consumption, and betel quid chewing, which are common harmful behaviors observed in certain cohorts of the Taiwanese population. Some reports have explored the potential therapeutic effect of certain herbal remedies on cancer treatments and the outcomes thereof. However, supportive evidence regarding the specific use of traditional Chinese medicine (TCM) in oral cancer treatment is lacking and deserves further investigation. This study measured the use of TCM therapies for oral cancer in a Taiwanese population-based retrospective longitudinal cohort study. The Taiwan National Health Insurance Research Database was utilized to conduct this study. The study population was limited to oral cancer patients diagnosed between 2000 and 2009, which were followed up for at least 5 years. Therapeutic strategies investigated included acupuncture and the Chinese herbs and formula used. Additionally, the frequency of TCM treatment visits, total medical costs, and all-cause mortality were also analyzed. Between 2000 and 2009, a total of 951 patients were diagnosed with various oral cancers. 13.7% of the diagnosed patients utilized TCM treatment measures. The majority of the patients were males. The top 3 common single herbs used were Xuán shēn (Radix Scrophulariae), Shí hú (Herba Dendrobii), and Mài mén dōng (Ophiopogon Japonicus). Then, Gān lù yǐn, Zhī bǎi dì huáng wán, and Sàn zhǒng kuì jiān tāng were the most frequently used herbal formulas. The survival probability was higher in TCM users when compared to non-TCM users in 5-and 12-year all-cause mortality (P < .05). This study explored the use of TCM therapies in oral cancer patients and identified essential information regarding the specifics of conventional herbal medicine used, affiliated medical costs, survival probability, and common symptoms observed in Taiwanese oral cancer patients.

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Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway

Cited by 20 publications

References 30 publications

Sodium Danshensu inhibits the progression of lung cancer by regulating PI3K/Akt signaling pathway

Sodium Danshensu inhibits the progression of lung cancer by regulating PI3K/Akt signaling pathway

PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition

Traditional Chinese medicine use in patients with oral cancer: A retrospective longitudinal cohort study in Taiwan

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