Sodium dodecyl sulfate-sensitive septation in a mitomycin C-sensitive, mtc, mutant of Escherichia coli

Higashi, Tomoyasu; Suzuki, Katsutoshi; Otsuji, Nozomu

doi:10.1128/jb.146.3.1117-1123.1981

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…The BL101 strain, which has an ethidiumsensitive phenotype (Ebs), derives from the AB1157 strain after a single mutation, located at 10 min on the bacterial chromosome (Lambert & Le Pecq, 1982). Several E. coli strains, with a mutation in the same region of the chromosome, have similar phenotypes (Imae, 1968;Nakamura, 1968;Otsuji, 1968;Tonnesen & Friesen, 1973;Nakamura et al, 1975;Coleman & Leive, 1979;Higashi et al, 1981). As discussed recently, all these mutations are probably allelic to acrA (Coleman & Leive, 1979).…”

Section: Resultsmentioning

confidence: 91%

Effect of mutation, electric membrane potential, and metabolic inhibitors on the accessibility of nucleic acids to ethidium bromide in Escherichia coli cells

Lambert¹,

Pecq²

1984

Biochemistry

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The uptake of ethidium bromide by Escherichia coli K 12 cells has been studied by using 14C-labeled ethidium and spectrofluorometry on three E. coli strains: the first one (AB1157) has an ethidium-resistant phenotype; the second one derives from the first one after a single mutation (at 10 min on the E. coli genetic map) and has an ethidium-sensitive (Ebs) phenotype; the third one is the acrA strain which appeared to have the same phenotype as the Ebs strain. When the cells are in exponential growth, no ethidium enters wild-type cells, and a very limited amount of ethidium enters Ebs and acrA cells. Massive quantities of ethidium enter AB1157, Ebs, and acrA cells treated by uncouplers and respiring Ebs cells treated by the membrane ATPase-inhibitor dicyclohexylcarbodiimide. A small amount of ethidium enters cells treated in M9 succinate medium by metabolic inhibitors such as KCN or cells starved with oxygen in the same M9 medium. The amount of ethidium and ethidium dimer retained at equilibrium by either type of cell, and by cells infected by T5 phage, as well as the kinetics of influx and efflux, has been measured under a variety of situations (membrane energized or not, and/or membrane ATPase inhibited or not). Furthermore, it was shown that ethidium binds to both RNA and DNA when it enters CCCP-treated wild-type E. coli cells, whereas it binds mainly to DNA when it enters Ebs and acrA cells in exponential growth. As it will be discussed, it is difficult to account for the EthBr uptake by invoking only membrane functions and active transport. Therefore, it is proposed that the variations of the nucleic acid accessibility in E. coli cells might play a role in the control of this uptake. Accordingly, in ethidium-sensitive cells, the mutation would have caused a significant part of the chromosomal DNA (10-20%) to become accessible to ethidium. Hansen [Hansen M. T. (1982) Mutat. Res. 106, 209-216], after a study of the photobinding of psoralen to nucleic acids in the acrA mutant, also suggested that DNA environment was modified in acrA cells.

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Section: Resultsmentioning

confidence: 91%

Effect of mutation, electric membrane potential, and metabolic inhibitors on the accessibility of nucleic acids to ethidium bromide in Escherichia coli cells

Lambert¹,

Pecq²

1984

Biochemistry

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“…Much activity has recently centered around the biophysics of cell membranes. This interest stems from the variety of processes in which the membrane plays a key role, among them cell shaping and movement [1], cell division [2], signal transduction [3], and molecule trafficking [4]. This latter process is of fundamental value for the regulation of the localization, assembly, and aggregation of molecules within, and in the vicinity of, the plasma membrane, functionalities that are all linked to a healthy cell existence [4].…”

Section: Introductionmentioning

confidence: 99%

Molecular motion in cell membranes: Analytic study of fence-hindered random walks

2008

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A theoretical calculation is presented to describe the confined motion of transmembrane molecules in cell membranes. The study is analytic, based on Master equations for the probability of the molecules moving as random walkers, and leads to explicit usable solutions including expressions for the molecular mean square displacement and effective diffusion constants. One outcome is a detailed understanding of the dependence of the time variation of the mean square displacement on the initial placement of the molecule within the confined region. How to use the calculations is illustrated by extracting (confinement) compartment sizes from experimentally reported published observations from single particle tracking experiments on the diffusion of gold-tagged G -protein coupled mu -opioid receptors in the normal rat kidney cell membrane, and by further comparing the analytical results to observations on the diffusion of phospholipids, also in normal rat kidney cells.

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“…The biophysics of cell membranes is an active field of current research, issues of interest being cell shaping and movement [1], cell division [2], signal transduction [3], and molecule trafficking [4]. Observations of the lateral movement of molecules on the surface of the cell [5][6][7][8][9][10][11] have given rise to the idea that the moving (transmembrane) molecules are confined within certain regions of the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Effects of disorder in location and size of fence barriers on molecular motion in cell membranes

Kalay

Parris²,

Kenkre

2008

J. Phys.: Condens. Matter

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The effect of disorder in the energetic heights and in the physical locations of fence barriers encountered by transmembrane molecules such as proteins and lipids in their motion in cell membranes is studied theoretically. The investigation takes as its starting point a recent analysis of a periodic system with constant distances between barriers and constant values of barrier heights, and employs effective medium theory to treat the disorder. The calculations make possible, in principle, the extraction of confinement parameters such as mean compartment sizes and mean intercompartmental transition rates from experimentally reported published observations. The analysis should be helpful both as an unusual application of effective medium theory and as an investigation of observed molecular movements in cell membranes.

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Sodium dodecyl sulfate-sensitive septation in a mitomycin C-sensitive, mtc, mutant of Escherichia coli

Cited by 3 publications

References 38 publications

Effect of mutation, electric membrane potential, and metabolic inhibitors on the accessibility of nucleic acids to ethidium bromide in Escherichia coli cells

Effect of mutation, electric membrane potential, and metabolic inhibitors on the accessibility of nucleic acids to ethidium bromide in Escherichia coli cells

Molecular motion in cell membranes: Analytic study of fence-hindered random walks

Effects of disorder in location and size of fence barriers on molecular motion in cell membranes

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