Sodium fluoride inhibits HSP synthesis in heat-stressed cultured cells of Arabidopsis thaliana

Pulyaevskaya, M. A.; Varakina, N. N.; Gamburg, K. Z.; Rusaleva, T. M.; Степанов, А. В.; Войников, В. К.; Rikhvanov, E. G.

doi:10.1134/s1021443711040108

Cited by 9 publications

(5 citation statements)

References 23 publications

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“…It was reported that shoots and leaves absorb fluoride from gaseous HF in the air near some industrial enterprises, while roots uptake fluoride from water and soil where fluoride can exist naturally or as a result of water fluoridation [ 20 ]. One study using Arabidopsis cell suspension showed no defects in cell viability after treatment with 20 mM NaF for 2 h [ 25 ]. However, even 1 mM NaF inhibited cell growth after 10 days [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…One study using Arabidopsis cell suspension showed no defects in cell viability after treatment with 20 mM NaF for 2 h [ 25 ]. However, even 1 mM NaF inhibited cell growth after 10 days [ 25 ]. Some plants even have an ability to uptake fluoride from the soil and convert it to the even more toxic fluoroacetate as a deterrent to consumption by animals [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Fluoride export (FEX) proteins from fungi, plants and animals are 'single barreled' channels containing one functional and one vestigial ion pore

et al. 2017

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The fluoride export protein (FEX) in yeast and other fungi provides tolerance to fluoride (F-), an environmentally ubiquitous anion. FEX efficiently eliminates intracellular fluoride that otherwise would accumulate at toxic concentrations. The FEX homolog in bacteria, Fluc, is a ‘double-barreled’ channel formed by dimerization of two identical or similar subunits. FEX in yeast and other eukaryotes is a monomer resulting from covalent fusion of the two subunits. As a result, both potential fluoride pores are created from different parts of the same protein. Here we identify FEX proteins from two multicellular eukaryotes, a plant Arabidopsis thaliana and an animal Amphimedon queenslandica, by demonstrating significant fluoride tolerance when these proteins are heterologously expressed in the yeast Saccharomyces cerevisiae. Residues important for eukaryotic FEX function were determined by phylogenetic sequence alignment and functional analysis using a yeast growth assay. Key residues of the fluoride channel are conserved in only one of the two potential fluoride-transporting pores. FEX activity is abolished upon mutation of residues in this conserved pore, suggesting that only one of the pores is functional. The same topology is conserved for the newly identified FEX proteins from plant and animal. These data suggest that FEX family of fluoride channels in eukaryotes are ‘single-barreled’ transporters containing one functional pore and a second non-functional vestigial remnant of a homologous gene fusion event.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fluoride export (FEX) proteins from fungi, plants and animals are 'single barreled' channels containing one functional and one vestigial ion pore

et al. 2017

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“…Morphological and anatomical symptoms: A cell suspension of Arabidopsis thaliana treated with 20 mM NaF for 2 h exhibited no loss of cell viability. However, cellular development was strictly inhibited by exposure to even 1 mM NaF for 10 d (Pulyaevskaya et al 2011). Fs can accumulate in the plant foliage and typical injury is marginal and tip necrosis gradually spread inward.…”

Section: Fluoride Phytotoxicitymentioning

confidence: 99%

Fluorine: a biohazardous agent for plants and phytoremediation strategies for its removal from the environment

Banerjee¹,

Roychoudhury²

2019

Biologia plant.

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Fluorine, one of the most abundant elements found on earth, acts as an environmental xenobiotics even at sparingly low concentrations. Uncontrolled anthropogenic activities have steeply increased the F content in the air, water, and soil. Irrigation of crops and vegetables with F contaminated groundwater or agricultural practices in contaminated soils adversely affect their physiological and biochemical parameters, leading to inhibited growth and productivity. Some plants can translocate the toxic ions from roots to shoots and accumulate them in the edible parts. Bioaccumulations of F have hazardous outcomes, as their concentrations in edible parts can be higher than the safe value. Screening of F hyperaccumulators and F-tolerant plants has been performed to facilitate phytoremediation. Some plant bioindicators have been identified which can be used to analyse the extent of atmospheric F pollution. Cumulative use of these organisms through proper scientific planning programs can potentially improve the agricultural soil quality in terms of their toxic F content. Future research should focus on proper execution of these phytoremediative strategies via robust field trials. High throughput genetic analyses should also be performed to identify quantitative trait loci which can be exploited to generate F-tolerant characters in susceptible crop cultivars.

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“…Таким образом, обработка в концентрации NaF 20 мМ приводит к сильному снижению жизнеспособности клеток A. thaliana. Эти данные подтверждают ранее полученные результаты [21], где было показано, что инкубация с 10 мМ NaF приводила к подавлению роста, однако на жизнеспособность практически не влияла, а увеличение концентрации до 20 мМ приводило к гибели культуры клеток A. thaliana. Что касается других видов растений, было показано, что концентрации 5 и 10 мМ NaF также вызывали значительное ингибирование роста побегов и корней таких растений, как нут, ячмень, редис, рожь, пшеница, люпин, подсолнечник, огурец, люцерна и томат [8].…”

Section: обсуждение результатовunclassified

Increased <i>Arabidopsis thaliana</i> cell culture resistance to sodium fluoride by constitutive expression of HSP101

Gorbyleva,

Safonova,

Stepanov

et al. 2023

Izvestiâ vuzov. Prikladnaâ himiâ i biotehnologiâ

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Fluorine is one of the toxic elemental components of industrial emissions. Increased fluoride content in the atmosphere, soil or water negatively affects the growth and development of plants, as well as reducing resistance to various environmental stressors. An increase in ambient temperature causes a protective response in all organisms taking the form heat shock protein synthesis. The specific protein HSP101, which performs the function of protecting plant cells from heat damage, is also responsible for inducible thermotolerance, representing the ability of organisms to withstand the effects of severe heat shock that were previously exposed to mild heat stress, as a result of which heat shock proteins are induced. Heat shock proteins are involved in protecting not only against elevated temperatures, but also various other stress factors. In this work, the effect of sodium fluoride treatment on the viability of Arabidopsis thaliana cell culture, expression and synthesis of heat shock proteins was studied along with the role of heat shock protein HSP101 in providing resistance to fluoride. Sodium fluoride has been shown to significantly reduce the viability of A. thaliana cells by suppressing the activation of HSP101 gene expression with an increase in temperature. At the same time, the A. thaliana line, which has constitutive expression of the HSP101 gene, proved to be more resistant to the toxic effects of sodium fluoride.

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Sodium fluoride inhibits HSP synthesis in heat-stressed cultured cells of Arabidopsis thaliana

Cited by 9 publications

References 23 publications

Fluoride export (FEX) proteins from fungi, plants and animals are 'single barreled' channels containing one functional and one vestigial ion pore

Fluoride export (FEX) proteins from fungi, plants and animals are 'single barreled' channels containing one functional and one vestigial ion pore

Fluorine: a biohazardous agent for plants and phytoremediation strategies for its removal from the environment

Increased <i>Arabidopsis thaliana</i> cell culture resistance to sodium fluoride by constitutive expression of HSP101

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