Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Abstract

Micropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first one, treatments consisted of control I (no sterilization), control II (thermal sterilization), and total active chlorine concentrations of 0.0005, 0.001, 0.002 and 0.003%. In the second experiment, based on the results observed in the first experiment, treatments consisted of control I (thermal sterilization) and II (chemical sterilization), and total active chlorine concentrations of 0.002, 0.0025 and 0.003%. Plant behavior was assessed based on the length of aerial part and roots, number of roots, and dry biomass of plants. Results showed that the addition of an active chlorine concentration of 0.003% to culture media provided total control of contaminants, and there were no significant differences regarding the variables analyzed between plants obtained with thermal sterilization and with sodium hypochlorite sterilization. Thus, chemical sterilization can be used as a replacement for thermal sterilization of nutrition media for rooting of gerbera in vitro.

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

“…At low active chlorine concentrations (up to 0.005%), sodium hypochlorite has proven viable in controlling bacterial manifestation in in vitro cultures of Eucalyptus benthamii at the establishment phase [16], Eucalyptus pellita at the multiplication phase [22], Sequoia sempervirens L. [21], the hybrid Gerbera hybrida cv. Essandre [24], Cochlospermum regium [25] and Bambusa vulgaris [26]. Our results for Eucalyptus mycrocoris agree with the literature: sodium hypochlorite at 0.003% produced the best response by eliminating microbial manifestations in 25% of individual 2 explants and 5% of individual 1 explants.…”

The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri

“…Clorox ® , a conventional bleach that consists of 5.25% of sodium hypochlorite (NaOCl), has the ability to penetrate the inner layers of the plant. It has been extensively used along with ethanol as an effective surface sterilizing agent in various plant species such as Ludisia discolour [41], Gerbera hybrida [42], Ananas comosus [43], Jatropha curcas [44], Aquilaria malaccensis [45], sour cherry [46] and Taraxacum belorussicum [47].…”

Use of chlorine dioxide to sterilize medium for tissue culture of potato

“…As low as 12.6 μM aqueous CD was capable of sterilizing potato culture media. This concentration was lower than the concentration of sodium hypochlorite (NaOCl) used to sterilize media in previous studies 17,18,31 , but similar to the CD concentrations used in other plant species 13–16 . Another advantage of using CD for sterilization is the low cost; the prices of sodium chlorite and food grade hydrochloric acid are far lower than commercial sodium hypochlorite products 11 .…”

“…The similar morphological features and rates of microtuber induction between autoclaved and CD sterilized media are in line with previous studies including Paris et al . 18 , while Teixeira et al . 17 and Cardoso & Silva 14 who reported that CD containing medium promoted the growth of plants.…”