Sodium ion modulates collagen types in human fibrolastic cells in culture

Hata, Ryu‐Ichiro; Sunada, Hironobu; Nagai, Yutaka

doi:10.1016/0006-291x(83)91577-2

Cited by 6 publications

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Activation of collagen synthesis in primary culture of rat liver parenchymal cells (hepatocytes)

Hata

Ninomiya

Sano

et al. 1985

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

An increase in collagen synthesis by hepatic parenchymal cells (hepatocytes) was observed during 8 days in primary culture by the quantification of total [3H]hydroxyproline as a marker of total collagen synthesis and the ratio of [3H]hydroxyproline in the high-molecular-weight fraction to total [3H]hydroxyproline as a marker of collagen degradation after incubation of the cells with [3H]proline for 24 h. Type analysis of the collagen produced by the cells after 8 days in culture showed the presence of type I and type III collagens in addition to the components corresponding to type IV and type V (alpha A and alpha B) collagens. Only the latter two types were found in the collagens produced by the cells after 2 days in primary culture. The purity of the hepatocytes inoculated was 97%, and the majority of the contaminating small cells were erythrocytes. The rate of serum albumin synthesis, which is a typical function of the hepatocytes, was constant or increased during the culture period. Immuno-electron microscopic observation indicated the production of type I collagen by the hepatocytes after 8 days in primary culture. These results are explained only by the activation of collagen synthesis in the day-8 hepatocytes in primary culture.

show abstract

Activation of collagen synthesis in primary culture of rat liver parenchymal cells (hepatocytes)

Hata

Ninomiya

Sano

et al. 1985

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

Reciprocal effects of excess potassium salts in the culture medium on type I and type III collagen synthesis by human skin fibroblasts

Hata¹,

Sunada²,

Arai³

1984

Cell Biology International Reports

View full text Add to dashboard Cite

By the addition of excess KCl or CH3COOK in the culture medium of human skin fibroblasts, production of non-collagenous proteins and membrane transport of [3H]proline of the cells were activated, but collagen synthesis was not increased. Production of type I collagen was inhibited under these culture conditions but type III collagen synthesis was activated; thus the ratio of type I to type III collagen decreased from 10 to near 1, indicating that the regulation of these two types of collagen is independent in human skin fibroblasts.

show abstract

Age-dependent changes in collagen metabolism and response to hypertonic culture conditions of rat aortic smooth muscle cells and skin fibroblasts

Hata

1990

Cell Biology International Reports

View full text Add to dashboard Cite

The rates of growth and collagen synthesis of rat aortic smooth muscle cells in culture were decreased with increasing the age of the animals from which the cells were obtained. These rates were further decreased by the presence of a 0.1 M excess concentration of KCl in the culture medium, and the cells obtained from older animals were much more sensitive to the inhibitory effect of the hypertonic culture condition. These tendencies were observed in smooth muscle cells obtained from different phases of in vitro cellular aging and also in skin fibroblasts obtained from animals of different ages. These results indicate that the cells become more sensitive to the environmental changes with increasing age both in vivo and in vitro.

show abstract

Sodium ion modulates collagen types in human fibrolastic cells in culture

Cited by 6 publications

References 12 publications

Activation of collagen synthesis in primary culture of rat liver parenchymal cells (hepatocytes)

Activation of collagen synthesis in primary culture of rat liver parenchymal cells (hepatocytes)

Reciprocal effects of excess potassium salts in the culture medium on type I and type III collagen synthesis by human skin fibroblasts

Age-dependent changes in collagen metabolism and response to hypertonic culture conditions of rat aortic smooth muscle cells and skin fibroblasts

Contact Info

Product

Resources

About