Sodium nitrite-induced oxidative stress causes membrane damage, protein oxidation, lipid peroxidation and alters major metabolic pathways in human erythrocytes

Ansari, Firoz Ahmad; Ali, Shaikh Nisar; Mahmood, Riaz

doi:10.1016/j.tiv.2015.07.022

Cited by 80 publications

(53 citation statements)

References 46 publications

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“…Percentage erythrocyte fragility was highest in the group II compared to group III (0.6 ± 0.03% vs 0.52 ± 0.05%). These findings are in agreement with previous reports [11]. Oxygen radicals are produced continuously in erythrocytes by hemoglobin autooxidation [44], and this process is accelerated upon exposure to a large number of xenobiotic agents [45,46].…”

Section: Resultssupporting

confidence: 93%

“…These data greatly supports and confirms that both nitrate and nitrite induce oxidative damage in rat erythrocytes. In accordance with this, recent studies [11,51] has reported a decrease in the levels of these enzymes in human erythrocytes treated with nitrite. In addition, it was observed that nitrite increased the activities of acid phosphatase, AMP deaminase, and LDH.…”

Section: T a B L E 1 Erythrocyte Oxidative Damage Biomarkers By Nitrsupporting

confidence: 80%

“…However, it has been reported that nitrite added to erythrocytes oxidizes glutathione and causes membrane damage, protein oxidation, high levels of lipid peroxidation [10] and also perturbs energy metabolism in these cells [11]. Besides, the administration of nitrite to mice leads to an increase in the metHb level, the activity of GR and glucose 6-phosphate dehydrogenase (G6PDH) of erythrocytes, as well as a decrease in the activity of SOD and CAT.…”

Section: +mentioning

confidence: 99%

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Nitrate and nitrite in drinking water affect antioxidant enzymes in erythrocytes of rats

Sierra-Campos¹,

Valdez-Solana²,

Campos-Almazán³

et al. 2018

Ukr.Biochem.J.

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D rinking water is derived from two basic sources: surface waters, such as rivers and reservoirs, and groundwater. All water contains natural contaminants, particularly inorganic compounds that arise from the geological strata through which water flows and, to a varying extent, anthropogenic pollution by both microorganisms and chemicals. The quality of drinking water and possible associated health risks vary throughout the world were some regions show high levels of arsenic and fluoride, or contamination by pathogens [1]. There is a number of possible sources of man-made contaminants, some of which are more important than others. Agriculture is another source of chemical contamination. In this case, the most important contaminant is nitrate, which can cause methemoglobinaemia, or blue-baby syndrome, in bottle-fed infants under 3 months of age [2].Comarca Lagunera Region of Mexico is famed as the largest milk-producing area. The Laguna faces a serious problem of over-exploitation and contamination of its water sources. Located in a semidesert, it produces cotton, alfalfa, walnut, cattle and goats. Such economic activities, along with the large production of milk and beer, consume the aquifer reserves in the region. As a result, the remaining water supplies are contaminated with arsenic, nitrates and other contaminants [3], an important factor for health problems in hundreds of people in the local communities.

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Section: Resultssupporting

confidence: 93%

Section: T a B L E 1 Erythrocyte Oxidative Damage Biomarkers By Nitrsupporting

confidence: 80%

Section: +mentioning

confidence: 99%

See 1 more Smart Citation

Nitrate and nitrite in drinking water affect antioxidant enzymes in erythrocytes of rats

Sierra-Campos¹,

Valdez-Solana²,

Campos-Almazán³

et al. 2018

Ukr.Biochem.J.

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“…28) What cannot be ignored is that nitrite and secondary amines, tertiary amines or amino compounds react to form strong carcinogenic nitrite amine compounds, which lead to cancers of the digestive system, chronic toxicity and teratogenic risks. [29][30][31] Furthermore, nitrite in the blood stream converts hemoglobin to methemoglobin, thereby interfering with the oxygen transport system of the body. 32) Excessive amounts of nitrite in food pose a serious threat to public health.…”

mentioning

confidence: 99%

The Synthesis and Evaluation of Arctigenin Amino Acid Ester Derivatives

Cai

Yang

Jia

et al. 2016

CHEMICAL & PHARMACEUTICAL BULLETIN

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The use of arctigenin (ARG), a traditional medicine with many pharmacological activities, has been restricted due to its poor solubility in water. Five amino acid derivatives of ARG have been synthesized using glycine, o-alanine, valine, leucine, and isoleucine, which have t-butyloxy carbonyl (BOC) as a protective group. In this study, we examined the effects of removing these protective groups. The results showed that the amino acid derivatives have better solubility and nitrite-clearing ability than ARG. Among the compounds tested, the amino acid derivatives without protective group were the best. Based on these results, ARG and its two amino acid derivatives without protective group (ARG8, ARG10) were selected to evaluate their anti-tumor activity in vivo at a dosage of 40 mg/kg. The results indicated that ARG8 and ARG10 both exhibit more anti-tumor activity than ARG in H 22 tumor-bearing mice. The tumor inhibition rates of ARG8 and ARG10 were 69.27 and 43.58%, which was much higher than ARG. Furthermore, the mice treated with these compounds exhibited less damage to the liver, kidney and immune organs compared with the positive group. Furthermore, ARG8 and ARG10 improved the serum cytokine levels significantly compared to ARG. In brief, this study provides a method to improve the water solubility of drugs, and we also provide a reference basis for new drug development.

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“…The lesions are similar to the results observed in our study. -Wakf et al, 2009) and Ansari et al (2015) (Ansari et al, 2015) reported that NaNO 2 induces protein and lipid oxidation, membrane damage, osmotic fragility, and morphological alterations in human erythrocytes. Their data suggested the creation of ROS and resultant production of oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

The potential role of mesenchymal stem cells in a hypoxia model induced by sodium nitrite in testes of male albino rats

et al. 2017

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Introduction: The present work aims to examine the possible role of stem cells on biochemical markers and histopathological alterations of hypoxia caused by sodium nitrite (NaNO 2 ) toxicity in testes of male rats. Methods: In this study, 96 adult male albino rats were divided into 6 groups (16 rats each). Group 1 (G1) was the control group and received distilled H 2 O. Group 2 (G2) received daily NaNO 2 (35 m/kg bwt/ day) via subcutaneous injection for 3 weeks. Group 3 (G3) received NaNO 2 for 2 weeks and were then injected once with 2*10 6 mesenchymal stem cells (MSCs) intravenously and sacrificed 4 weeks later. Group 4 (G4) received NaNO 2 for 2 weeks and were then injected with 2*10 6 MSCs followed by daily NaNO 2 injection for 1 week; rats in G4 were sacrificed 4 weeks from MSCs treatment. Group 5 (G5) rats were treated with NaNO 2 for 2 weeks and then left to recover for 4 weeks. Finally, Group 6 (G6) rats were treated with NaNO 2 for 3 weeks and left to recover for 3 weeks, after which point they were sacrificed. Results: The results showed that NaNO 2 caused oxidative damage and histopathological alterations in the rat testes, as well as increased the levels of testes malondialdehyde (MDA), nitric oxide (NO) and DNA fragmentation percentage (DNA F %). Moreover, NaNO 2 decreased the elevated activities of testes catalase (CAT) and total antioxidant activity (TAA), in comparison to the control group. The histological results illustrated different distortions, vacuolization and lipid accumulations in interlobular space as well as diminution of inter cellular germ cell layers, absence of Leydig cells, irregular basement membrane of tubule, and separation within spermatogenic cells. In

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Sodium nitrite-induced oxidative stress causes membrane damage, protein oxidation, lipid peroxidation and alters major metabolic pathways in human erythrocytes

Cited by 80 publications

References 46 publications

Nitrate and nitrite in drinking water affect antioxidant enzymes in erythrocytes of rats

Nitrate and nitrite in drinking water affect antioxidant enzymes in erythrocytes of rats

The Synthesis and Evaluation of Arctigenin Amino Acid Ester Derivatives

The potential role of mesenchymal stem cells in a hypoxia model induced by sodium nitrite in testes of male albino rats

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