Sodium pheophorbide a has photoactivated fungicidal activity against Pestalotiopsis neglecta

Yang, Jing; Lin, Lian-Nan; Zhang, Xiaobo; Yue, Wu; Bing, Bi; Zhang, Guocai; Zou, Chuanshan

doi:10.1016/j.pestbp.2019.04.003

Cited by 17 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The control effect of strain La2 on pear Valsa canker was assessed based on a previous method. 18 In short, healthy and uniformly growing annual pear twigs were selected and punctured by using a sterile borer (0.5 cm in diameter), then 30 μL of conidial suspension (1 × 10 8 CFU mL −1 ) of strain La2 was placed in each hole. Sterile water was used as the negative control and 5000× diluted tebuconazole was used as a positive control.…”

Section: Control Effect Of Strain La2 On Pear Valsa Cankermentioning

confidence: 99%

Biocontrol activity and potential mechanism of volatile organic compounds from Aspergillus niger strain La2 against pear Valsa canker

Shi,

Yuan,

Wang

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

BACKGROUNDValsa pyri‐caused pear Valsa canker is one of the most destructive diseases of pear, causing severe yield and economic lost. Volatile organic compounds (VOCs) from endophytes take important roles in the regulation of plant disease. In the present study, we investigated the biocontrol activity of endophytic fungus Aspergillus niger strain La2 and its antagonistic VOCs against pear Valsa canker.RESULTSThe strain La2 exhibited obvious inhibitory effect against V. pyri. Colonization assay suggested that strain La2 could colonize to complete its life cycle on pear twigs. The symptoms of pear Valsa canker were weakened on detached pear twigs after treatment with strain La2. In addition, VOCs from strain La2 also significantly suppressed the mycelial growth of V. pyri. Based on the results of headspace solid‐phase microextraction/gas chromatography‐mass spectrometry (HS‐SPME‐GC‐MS) analysis, 6 possible VOCs produced by strain La2 were detected, and 2,4‐di‐tert‐butylphenol and 4‐methyl‐1‐pentanol were the main antagonistic VOCs of strain La2 according to its antagonism effect on pear Valsa canker in vitro and in vivo. Further results showed that 4‐methyl‐1‐pentanol could destroy the V. pyri hypha structure and cell membrane integrity. Importantly, the activities of pear defense‐related enzymes (PPO, PAL and SOD) were enhanced after 4‐methyl‐1‐pentanol treatment in pear twigs, implying that 4‐methyl‐1‐pentanol might induce plant disease resistance response.CONCLUSIONA. niger strain La2 and its VOCs compounds 2,4‐di‐tert‐butylphenol and 4‐methyl‐1‐pentanol offer the potential as novel biocontrol agents of pear Valsa canker.This article is protected by copyright. All rights reserved.

show abstract

Section: Control Effect Of Strain La2 On Pear Valsa Cankermentioning

confidence: 99%

Biocontrol activity and potential mechanism of volatile organic compounds from Aspergillus niger strain La2 against pear Valsa canker

Shi,

Yuan,

Wang

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…11 Pheophorbide-a can generate singlet oxygen upon excitation under red light. 12 It is assumed that this singlet oxygen breaks the phosphodiester bond in the liposome through further oxidation to destroy the liposome structure. Thus, the leakage of the liposome-encapsulated drug can control the drug's release (Figure 1).…”

Section: ■ Introductionmentioning

confidence: 99%

“…It can be used as an excellent photosensitizer and has broad application prospects in light-responsive nanodrug carriers . Pheophorbide-a can generate singlet oxygen upon excitation under red light . It is assumed that this singlet oxygen breaks the phosphodiester bond in the liposome through further oxidation to destroy the liposome structure.…”

Section: Introductionmentioning

confidence: 99%

Pheophorbide-a as a Light-Triggered Liposomal Switch: For the Controlled Release of Alpinia galanga (A. galanga) Essential Oil and Its Stability, Antioxidant, and Antibacterial Activity Assessment

Shen

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

In this study, Alpinia galanga essential oil liposomes (EO-Lip) were prepared with soybean lecithin and cholesterol as wall materials. A light-responsive liposome (EO-PLip) was designed for the controlled release of A. galanga oil based on the lightresponsive properties of Pheophorbide-a. The dependence of Pheophorbide-a on illumination time was proved by UV spectroscopy. Characterization techniques such as UV spectroscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy demonstrated that the essential oils were successfully encapsulated in liposomes. Moreover, the particle size of EO-PLip was 166.30 nm, the polydispersity index was 0.22, the zeta potential was −49.50 mV, and the encapsulation efficiency was 30.83%. Both EO-Lip and EO-Plip have high sustained-release effects on essential oil and showed light-responsive release characteristics under infrared stimulation. The prepared liposomes had good storage stability at 4 °C for 28 d. EO-PLip showed excellent transient antioxidant and bacteriostatic properties based on the ability to respond to light and slow release. This EO-PLip provided a platform for essential oils and might be used as a potent and controllable solution.

show abstract

“…Nowadays, the use of chemical pesticides is still the main means of controlling various diseases. A study suggests that Sodium pheophorbide a (SPA) inhibits spore germination and has detrimental effects on growth, cell wall, membrane permeability, morphology, and enzyme activities of P. neglecta mycelia, thereby disturbing the functions of the fungus and strongly inhibiting its growth [17]. Transcriptomic analysis showed most DEGs were involved in the metabolism of amino acids, carbohydrates, and lipids, as well as cell structure and genetic information processing [18].…”

Section: Introductionmentioning

confidence: 99%

“…High-quality transcriptomes have been sequenced from P. trachicarpicola-infected tea leave and acquire a clue to the infection-related gene [15]. Transcriptomic analysis has been employed to unravel potential pathways and genes involved in pecan (Carya illinoinensis) resistance to P. microspora [17]. The P. neglecta's response to sodium pheophorbide a (SPA) and cell wall degrading enzyme-related gene was revealed in the transcriptomic analysis [25].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Mechanism of Phenazine-1-Carboxylic Acid against Pestalotiopsis kenyana

Xun

Gong

Liu

et al. 2023

IJMS

View full text Add to dashboard Cite

Pestalotiopsis sp. is an important class of plant pathogenic fungi that can infect a variety of crops. We have proved the pathogenicity of P. kenyana on bayberry leaves and caused bayberry blight. Phenazine-1-carboxylic acid (PCA) has the characteristics of high efficiency, low toxicity, and environmental friendliness, which can prevent fungal diseases on a variety of crops. In this study, the effect of PCA on the morphological, physiological, and molecular characteristics of P. kenyana has been investigated, and the potential antifungal mechanism of PCA against P. kenyana was also explored. We applied PCA on P. kenyana in vitro and in vivo to determine its inhibitory effect on PCA. It was found that PCA was highly efficient against P. kenyana, with EC50 around 2.32 μg/mL, and the in vivo effect was 57% at 14 μg/mL. The mechanism of PCA was preliminarily explored by transcriptomics technology. The results showed that after the treatment of PCA, 3613 differential genes were found, focusing on redox processes and various metabolic pathways. In addition, it can also cause mycelial development malformation, damage cell membranes, reduce mitochondrial membrane potential, and increase ROS levels. This result expanded the potential agricultural application of PCA and revealed the possible mechanism against P. kenyana.

show abstract

Sodium pheophorbide a has photoactivated fungicidal activity against Pestalotiopsis neglecta

Cited by 17 publications

References 34 publications

Biocontrol activity and potential mechanism of volatile organic compounds from Aspergillus niger strain La2 against pear Valsa canker

Biocontrol activity and potential mechanism of volatile organic compounds from Aspergillus niger strain La2 against pear Valsa canker

Pheophorbide-a as a Light-Triggered Liposomal Switch: For the Controlled Release of Alpinia galanga (A. galanga) Essential Oil and Its Stability, Antioxidant, and Antibacterial Activity Assessment

Antifungal Mechanism of Phenazine-1-Carboxylic Acid against Pestalotiopsis kenyana

Contact Info

Product

Resources

About