Sodium Salicylate Inhibits Urokinase Activity in MDA MB-231 Breast Cancer Cells

Madunić, Josip; Horvat, Luka; Majstorović, Ivana; Jodłowska, Iga; Antica, Mariastefania; Matulić, Maja

doi:10.1016/j.clbc.2017.03.015

Cited by 12 publications

(11 citation statements)

References 55 publications

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“…1d). The selective effects of SA and ASA on cancer cells has been demonstrated elsewhere and is compatible with the clinical use of these drugs to treat many diseases 25,26 . Moreover, these results show that the effects of the drugs are not specific to B16F10 melanoma cells but also affect other cancer cells lines, which has been shown elsewhere 27 .…”

Section: Resultssupporting

confidence: 60%

Acetylsalicylic acid and salicylic acid present anticancer properties against melanoma by promoting nitric oxide-dependent endoplasmic reticulum stress and apoptosis

Ausina

Branco

Demaria

et al. 2020

Sci Rep

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Melanoma is the most aggressive and fatal type of skin cancer due to being highly proliferative. Acetylsalicylic acid (ASA; Aspirin) and salicylic acid (SA) are ancient drugs with multiple applications in medicine. Here, we showed that ASA and SA present anticancer effects against a murine model of implanted melanoma. These effects were also validated in 3D- and 2D-cultured melanoma B16F10 cells, where the drugs promoted pro-apoptotic effects. In both in vivo and in vitro models, SA and ASA triggered endoplasmic reticulum (ER) stress, which culminates with the upregulation of the pro-apoptotic transcription factor C/EBP homologous protein (CHOP). These effects are initiated by ASA/SA-triggered Akt/mTOR/AMPK-dependent activation of nitric oxide synthase 3 (eNOS), which increases nitric oxide and reactive oxygen species production inducing ER stress response. In the end, we propose that ASA and SA instigate anticancer effects by a novel mechanism, the activation of ER stress.

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Section: Resultssupporting

confidence: 60%

Acetylsalicylic acid and salicylic acid present anticancer properties against melanoma by promoting nitric oxide-dependent endoplasmic reticulum stress and apoptosis

Ausina

Branco

Demaria

et al. 2020

Sci Rep

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“…This suggests that aspirin is a promising chemopreventive agent and it is not only anti-inflammatory but also anticarcinogenic. These anticancer properties have also been exhibited in human breast cancer MDA-MB-231 cells [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages

Hsieh

Wang

2018

Mediators of Inflammation

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The tumor microenvironment is rich in multiple cell types that influence tumor development. Macrophages infiltrate tumors, where they are the most abundant immune cell population and secrete a number of cytokines. Aspirin acts as a chemopreventive agent against cancer development. This study investigated whether aspirin regulates crosstalk between breast cancer cells and macrophages. To study these interactions in a tumor microenvironment, a conditioned media was employed using 4T1 breast cancer cells cultured in RAW 264.7 cell-conditioned medium (RAW-CM), and a cocultured model of both cells was used. When 4T1 cells were cultured in the RAW-CM, there were increases in cell viability and secretion of the cytokines VEGF, PAI-1, TNF-α, and IL-6. Treatment with aspirin inhibited 4T1 cell growth and migration and MCP-1, PAI-1, and IL-6 production. In the coculture of both cells, aspirin inhibited secretion of MCP-1, IL-6, and TGF-β. Furthermore, aspirin significantly decreased the M2 macrophage marker CD206, but increased M1 marker CD11c expression. In summary, aspirin treatment inhibited the crosstalk of 4T1 and RAW 264.7 cells through regulation of angiogenic and inflammatory mediator production and influenced the M1/M2 macrophage subtype. This highlighted that aspirin suppresses the tumor favorable microenvironment and could be a promising agent against triple-negative breast cancer.

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“…This inhibition may associate with the salicylic acid group. During the process of breast cancer metastasis, aspirin can resist platelet activation and thus inhibit platelet-induced epithelial transformation, migration and invasion ability of breast cancer cells 12 . Asprin can also inhibit the activation of the transcription factor-1 (AP-1) and the nuclear factor κB (NF-κB), thus affecting cell proliferation, differentiation and apoptosis, transformation, invasion and metastasis of tumor cells 13 .…”

Section: Introductionmentioning

confidence: 99%

Zinc Complex of 3,5-di-tert-butyl Salicylate Inhibits the Proliferation, Migration, and Invasion of Triple-Negative Breast Cancer Cells

Chen

Wang

Fan

et al. 2021

Preprint

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Zinc complex of 3,5-di-tert-butyl salicylate (Zn{[CH3)3C]2Sal}22-) is a zinc ion chelate of salicylate. In this study, we found that it inhibits the proliferation, invasion, migration and induce apoptosis of triple-negative breast cancer 4T1 cells. The results of RNA-seq showed that expression of 17 genes was up-regulated and 26 genes was down-regulated significantly by Zn{[CH3)3C]2Sal}22- treatment. Further GO analysis and KEGG analysis showed that the activity of Zn{[CH3)3C]2Sal}22- against triple-negative breast cancer cells may be involved JAK-STAT3, HIF-1, and TNF signaling pathways. The expression of key genes was verified by RT-PCR. The phosphorylation of STAT3 and its upstream SRC decreased drastically upon Zn{[CH3)3C]2Sal}22- treatment as analyzed by western blot. Our results indicate that Zn{[CH3)3C]2Sal}22- inhibits the activity of TNBC cells by down-regulating STAT3 signaling pathway.

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Sodium Salicylate Inhibits Urokinase Activity in MDA MB-231 Breast Cancer Cells

Cited by 12 publications

References 55 publications

Acetylsalicylic acid and salicylic acid present anticancer properties against melanoma by promoting nitric oxide-dependent endoplasmic reticulum stress and apoptosis

Acetylsalicylic acid and salicylic acid present anticancer properties against melanoma by promoting nitric oxide-dependent endoplasmic reticulum stress and apoptosis

Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages

Zinc Complex of 3,5-di-tert-butyl Salicylate Inhibits the Proliferation, Migration, and Invasion of Triple-Negative Breast Cancer Cells

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