Sodium Selenite inhibits mitophagy, downregulation and mislocalization of blood-testis barrier proteins of bovine Sertoli cell exposed to microcystin-leucine arginine (MC-LR) via TLR4/NF-kB and mitochondrial signaling pathways blockage

Adegoke, Elikanah Olusayo; Wang, Handong; Machebe, N. S.; Adeniran, S.O.; Wang, Hao; Chen, Wang; Han, Zhang; Zhang, Guixue; Zheng, Ping

doi:10.1016/j.ecoenv.2018.09.073

Cited by 33 publications

(20 citation statements)

References 72 publications

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“…In fact, this effect of Se was dose-dependent and exhibited the U-shaped response i.e., optimal ameliorative effects were observed at moderate doses (0.25, 0.50), and temporary cytotoxic effects were evident when the dose was increased to 0.75, and 1.00 mg/L [97]. Interestingly, the mechanistic basis for these effects of Se was later elucidated by the same group of researchers [98], where they demonstrated that Se-pretreatment could ameliorate microcystin-LR (MC-LR)-triggered cytotoxicity in bovine Sertoli cells. Pre-treatment with inorganic Se (0.50 mg/L) inhibited the nuclear factor kappa B (NFκB) activation in cultured Sertoli cells.…”

Section: Role Of Selenium In Male Reproductionmentioning

confidence: 99%

“…Pre-treatment with inorganic Se (0.50 mg/L) inhibited the nuclear factor kappa B (NFκB) activation in cultured Sertoli cells. Selenium also exhibited some immunomodulatory roles, which were evident by the inhibition of inflammatory cytokine activation in MC-LR-exposed Sertoli cells [98]. Besides, Se-pretreatment also modulated the expression of mitochondria-related genes such as Cytochrome c oxidase subunit I ( COX-1 ), Cytochrome c oxidase subunit 2 (COX-2), Acetyl-CoA acetyltransferase 1 (ACAT1), Mitochondrial transcription factor A ( mtTFA ) and Subunit 2 of NADH dehydrogenase ( MT-ND2 ).…”

Section: Role Of Selenium In Male Reproductionmentioning

confidence: 99%

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Role of Selenium and Selenoproteins in Male Reproductive Function: A Review of Past and Present Evidences

et al. 2019

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Selenium (Se) is an important trace mineral having many essential roles at the cellular and organismal levels in animal and human health. The biological effects of Se are mainly carried out by selenoproteins (encoded by 25 genes in humans and 24 in mice). As an essential component of selenoproteins, Se performs structural and enzymic roles; in the latter context it is well known for its catalytic and antioxidative functions. Studies involving different animal models have added great value to our understanding regarding the potential implications of Se and selenoproteins in mammalian fertility and reproduction. In this review, we highlight the implications of selenoproteins in male fertility and reproduction followed by the characteristic biological functions of Se and selenoproteins associated with overall male reproductive function. It is evident from observations of past studies (both animal and human) that Se is essentially required for spermatogenesis and male fertility, presumably because of its vital role in modulation of antioxidant defense mechanisms and other essential biological pathways and redox sensitive transcription factors. However, bearing in mind the evidences from mainstream literature, it is also advisable to perform more studies focusing on the elucidation of additional roles played by the peculiar and canonical selenoproteins i.e., glutathione peroxidase 4 (GPX4) and selenoprotein P (SELENOP) in the male reproductive functions. Nevertheless, search for the elucidation of additional putative mechanisms potentially modulated by other biologically relevant selenoproteins should also be included in the scope of future studies. However, as for the implication of Se in fertility and reproduction in men, though a few clinical trials explore the effects of Se supplementation on male fertility, due to inconsistencies in the recruitment of subjects and heterogeneity of designs, the comparison of such studies is still complicated and less clear. Therefore, further research focused on the roles of Se and selenoproteins is awaited for validating the evidences at hand and outlining any therapeutic schemes intended for improving male fertility. As such, new dimensions could be added to the subject of male fertility and Se supplementation.

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Section: Role Of Selenium In Male Reproductionmentioning

confidence: 99%

Section: Role Of Selenium In Male Reproductionmentioning

confidence: 99%

Role of Selenium and Selenoproteins in Male Reproductive Function: A Review of Past and Present Evidences

et al. 2019

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“…Previous studies on MC‐LR (Adamovsky et al, ; Adegoke, Wang, Machebe, Adeniran, et al, ; Adegoke, Wang, Machebe, Wang, et al, ) indicated that MC‐LR elicit its toxic effects via TLR4 signaling pathways. Therefore, the TLR4 signaling pathway was made the target for amelioration and attenuation of MC‐LR toxicity in this study.…”

Section: Discussionmentioning

confidence: 98%

“…Western blot analysis was performed as described by Adegoke et al (). Briefly, C34 pretreated and non‐pretreated Sertoli cells exposed to MC‐LR for 24 hours were harvested, washed with PBS, lysed in RIPA buffer (Santa Cruz, Santa Cruz, CA, USA) containing a protease inhibitor cocktail (Santa Cruz) for 10 minutes at room temperature and centrifuged at 12 000 g for 10 minutes, to remove cellular debris.…”

Section: Methodsmentioning

confidence: 99%

Pharmacological inhibition of TLR4/NF‐κB with TLR4‐IN‐C34 attenuated microcystin‐leucine arginine toxicity in bovine Sertoli cells

Adegoke

Adeniran

Zeng

et al. 2019

J of Applied Toxicology

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This study investigated the pharmacological inhibition of the toll‐like receptor 4 (TLR4) genes as a measure to attenuate microcystin‐LR (MC‐LR) reproductive toxicity. Bovine Sertoli cells were pretreated with TLR4‐IN‐C34 (C34) for 1 hour. Thereafter the pretreated and non‐pretreated Sertoli cells were cultured in medium containing 10% heat‐activated fetal bovine serum + 80 μg/L MC‐LR for 24 hours to assess the ability of TLR4‐IN‐C34 to attenuate the toxic effects of MC‐LR. The results showed that TLR4‐IN‐C34 inhibited MC‐LR‐induced mitochondria membrane damage, mitophagy and downregulation of blood‐testis barrier constituent proteins via TLR4/nuclear factor‐kappaB and mitochondria‐mediated apoptosis signaling pathway blockage. The downregulation of the mitochondria electron transport chain, energy production and DNA replication related genes (mt‐ND2, COX‐1, COX‐2, ACAT, mtTFA) and upregulation of inflammatory cytokines (interleukin [IL]‐6, tumor necrosis factor‐α, IL‐1β, interferon‐γ, IL‐4, IL‐10, IL‐13 and transforming growth factor β1) were modulated by TLR4‐IN‐C34. Taken together, we conclude that TLR4‐IN‐C34 inhibits MC‐LR‐related disruption of mitochondria membrane, mitophagy and downregulation of blood‐testis barrier proteins of the bovine Sertoli cell via cytochrome c release and TLR4 signaling blockage.

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“…It may be that some components of fatal bovine serum (FBS) in thawing solution affected sperm activation, sperm motility, and metabolism. FBS contains many bioactive substances, which are commonly used nutrients and antifreeze protectors in culture and freezing of embryo and cell [30]. Therefore, it is necessary to further verify the effects of FBS on sperm motility, survival time, metabolic mode, and fertilization ability at room temperature, low temperature, and ultralow temperature.…”

Section: Discussionmentioning

confidence: 99%

DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance

Zheng¹,

Li²,

Huang³

et al. 2020

Turk J Vet Anim Sci

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The purpose of this experiment was to investigate the effects of Beltsville thawing solution (BTS), Androhep, and Dulbecco's modified Eagle medium + Fatal bovine serum (D-F) as diluents of frozen semen on sperm motility and sow reproductive performance. In experiment 1, boar semen was collected and diluted to 2.0 × 10 9 /60 mL, 1.5 × 10 9 /60 mL, and 1.5 × 10 9 /40 mL with Androhep. The appropriate semen dose was determined by postcervical insemination. In experiment 2, boar semen was fumigated with liquid nitrogen and thawed semen was diluted to 1.5 × 10 9 /40 mL with D-F, Androhep, and BTS, respectively. The effects of above diluents on sperm motility and sow reproductive performance were studied by postcervical insemination. The appropriate semen dose for postcervical insemination was 1.5 × 10 9 /40 mL. After thawing of frozen semen, sperm motility of semen diluted with D-F was significantly higher than that of semen diluted with Androhep and BTS. There was no significant difference in pregnancy rates among sows via artificial insemination. The litter size of sows using D-F diluted semen was significantly higher than that of sows using Androhep and BTS diluted semen. In conclusion, D-F freezing diluent can improve sperm motility of frozen boar semen after thawing, prolong sperm survival time, and increase sow reproductive performance.

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Sodium Selenite inhibits mitophagy, downregulation and mislocalization of blood-testis barrier proteins of bovine Sertoli cell exposed to microcystin-leucine arginine (MC-LR) via TLR4/NF-kB and mitochondrial signaling pathways blockage

Cited by 33 publications

References 72 publications

Role of Selenium and Selenoproteins in Male Reproductive Function: A Review of Past and Present Evidences

Role of Selenium and Selenoproteins in Male Reproductive Function: A Review of Past and Present Evidences

Pharmacological inhibition of TLR4/NF‐κB with TLR4‐IN‐C34 attenuated microcystin‐leucine arginine toxicity in bovine Sertoli cells

DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance

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