Soehngenia saccharolytica gen. nov., sp. nov. and Clostridium amygdalinum sp. nov., two novel anaerobic, benzaldehyde-converting bacteria

Parshina, Sofiya N.

doi:10.1099/ijs.0.02668-0

Cited by 84 publications

(34 citation statements)

References 41 publications

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“…The presence of Ottowia together with Advenella Ghosh et al 2011), Corynebacterium (Dalal et al 2012;Guo et al 2015), and Sphingobium (Kertesz and Kawasaki 2010) might be associated with the removal of phenols, NHCs as well as PAHs under the anaerobic conditions (Table S8). Soehngenia, the second most abundant anaerobic bacterium in this study, was previously reported to degrade benzaldehyde (Parshina et al 2003), an intermediate in anaerobic phenol degradation (Sharma and Philip 2015). Hydrolytic fermentative acidogenic activity in anaerobic bioreactors is likely controlled by strict anaerobic bacteria like Longilinea, Clostridium, and Proteiniphilum (Yamada et al 2007;Kohda et al 1997;Letowski et al 2001;Chen and Dong 2005;Larsen et al 2009) and facultative aerobic bacteria such as Corynebacterium (Kampfer et al 2009).…”

Section: Discussionmentioning

confidence: 76%

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Joshi

Zhang

Tian

et al. 2016

Appl Microbiol Biotechnol

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The combined anaerobic-aerobic biosystem is assumed to consume less energy for the treatment of high strength industrial wastewater. In this study, pollutant removal performance and microbial diversity were assessed in a longterm (over 300 days) bench-scale sequential anaerobicaerobic bioreactor treating coking wastewater. Anaerobic treatment removed one third of the chemical oxygen demand (COD) and more than half of the phenols with hydraulic retention time (HRT) of 42 h, while the combined system with total HRT of 114 h removed 81.8, 85.6, 99.9, 98.2, and 85.4 % of COD, total organic carbon (TOC), total phenols, thiocyanate, and cyanide, respectively. Two-dimensional gas chromatography with time-of-flight mass spectrometry showed complete removal of phenol derivatives and nitrogenous heterocyclic compounds (NHCs) via the combined system, with the anaerobic process alone contributing 58.4 and 58.6 % removal on average, respectively. Microbial activity in the bioreactors was examined by 454 pyrosequencing of the bacterial, archaeal, and fungal communities. Proteobacteria (61.2-93.4 %), particularly Betaproteobacteria (34.4-70.1 %), was the dominant bacterial group. Ottowia (14.1-46.7 %), Soehngenia (3.0-8.2 %), and Corynebacterium (0.9-12.0 %), which are comprised of phenol-degrading and hydrolytic bacteria, were the most abundant genera in the anaerobic sludge, whereas Thiobacillus (6.6-43.6 %), Diaphorobacter (5.1-13.0 %), and Comamonas (0.2-11.1 %) were the major degraders of phenol, thiocyanate, and NHCs in the aerobic sludge. Despite the low density of fungi, phenol degrading oleaginous yeast Trichosporon was abundant in the aerobic sludge. This study demonstrated the feasibility and optimization of less energy intensive treatment and the potential association between abundant bacterial groups and biodegradation of key pollutants in coking wastewater.

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Section: Discussionmentioning

confidence: 76%

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Joshi

Zhang

Tian

et al. 2016

Appl Microbiol Biotechnol

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“…Medium preparation and analytical methods used were as described by Parshina et al (2003Parshina et al ( , 2005. CO 2 analysis was performed according to Henstra & Stams (2004).…”

Section: Desulfotomaculum Nigrificans Dsm 574mentioning

confidence: 99%

Desulfotomaculum carboxydivorans sp. nov., a novel sulfate-reducing bacterium capable of growth at 100 % CO

Parshina

Sipma

Nakashimada

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

107

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A moderately thermophilic, anaerobic, chemolithoheterotrophic, sulfate-reducing bacterium, strain CO-1-SRB T , was isolated from sludge from an anaerobic bioreactor treating paper mill wastewater. Cells were Gram-positive, motile, spore-forming rods. The temperature range for growth was 30-68 6C, with an optimum at 55 6C. The NaCl concentration range for growth was 0-17 g l "1; there was no change in growth rate until the NaCl concentration reached 8 g l . The pH range for growth was 6?0-8?0, with an optimum of 6?8-7?2. The bacterium could grow with 100 % CO in the gas phase. With sulfate, CO was converted to H 2 and CO 2 and part of the H 2 was used for sulfate reduction; without sulfate, CO was completely converted to H 2 and CO 2 . With sulfate, strain CO-1-SRB T utilized H 2 /CO 2 , pyruvate, glucose, fructose, maltose, lactate, serine, alanine, ethanol and glycerol. The strain fermented pyruvate, lactate, glucose and fructose. Yeast extract was necessary for growth. Sulfate, thiosulfate and sulfite were used as electron acceptors, whereas elemental sulfur and nitrate were not. A phylogenetic analysis of 16S rRNA gene sequences placed strain CO-1-SRB T in the genus Desulfotomaculum, closely resembling Desulfotomaculum nigrificans DSM 574 T and Desulfotomaculum sp. RHT-3 (99 and 100 % similarity, respectively). However, the latter strains were completely inhibited above 20 and 50 % CO in the gas phase, respectively, and were unable to ferment CO, lactate or glucose in the absence of sulfate. DNA-DNA hybridization of strain CO-1-SRB T with D. nigrificans and Desulfotomaculum sp. RHT-3 showed 53 and 60 % relatedness, respectively. On the basis of phylogenetic and physiological features, it is suggested that strain CO-1-SRB T represents a novel species within the genus Desulfotomaculum, for which the name Desulfotomaculum carboxydivorans is proposed. This is the first description of a sulfate-reducing micro-organism that is capable of growth under an atmosphere of pure CO with and without sulfate. The type strain is CO-1-SRB T (=DSM 14880 T =VKM B-2319 T ).

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“…Moreover, in Trypticase-yeast extract broth (without glucose) supplemented with 10 mmol of benzaldehyde/liter, it forms benzyl alcohol in approximately equal amounts as determined by gas chromatography (data not shown). To our knowledge, this property has been reported only for two species previously described, namely, Clostridium amygdalinum and Soehngenia saccharolytica (11), and for Clostridium acetobutylicum (9). However, a concentration of 10 mmol of benzaldehyde/liter inhibited the growth of C. acetobutylicum.…”

mentioning

confidence: 72%

“…Alignments were done with CLUSTAL W (13). The phylogenetic tree revealed that strain AIP 321.05 falls into cluster XIVa of Clostridium (7), with highest sequence similarity (99.3%) to an environmental strain, namely, Clostridium amygdalinum (11) (Fig. 1).…”

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confidence: 99%

First Isolation of Clostridium amygdalinum from a Patient with Chronic Osteitis

et al. 2006

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We describe a case of osteitis caused by a new and unusual Clostridium species, Clostridium amygdalinum, an environmental, moderately thermophilic bacterium. This is the first documented report of human infection caused by this organism. CASE REPORTA 34-year-old man sustained in November 2003 a bifocal open fracture of the right tibial shaft (rated Gustilo IIIB). The index trauma was a motorcycle accident, and the patient's open leg fracture stayed in contact with the ground for about 6 h before he was admitted to the primary care institution. In the emergency unit, the fracture was treated by external fixation, and the wound was closed with the help of plastic surgery (local muscle flap and skin graft). The patient was admitted to our institution in October 2005 because of septic nonunion of the right tibial shaft despite the fact that the external fixation was maintained. The diagnosis of septic nonunion was reached due to the two productive fistulas located at the anterior aspect of the leg combined with failure of external fixation and the persistence of the fracture on X-ray images. At that time, surgical management consisted of setting up a new extended external fixation combined with a surgical excision at the level of the right tibia (soft tissue debridement and removal of 10 cm of necrotic tibial bone). During that surgery, 14 biopsy specimens were obtained. Eight of them revealed after culture the presence of Clostridium sporogenes and four showed a grampositive, strictly anaerobic, motile, spore-forming rod, designated AIP 321.05. Bacterial growth was not seen in two biopsy specimens. The patient was then treated with linezolid (600 mg twice daily) for 2 months. Two months later, the patient had hyperthermia, and no bony consolidation was observed. A second reconstructive surgery was performed, consisting of a vascularized fibular bone graft. Several biopsies were performed. Two revealed after culture only the presence of the same unidentified strain AIP 321.05, as evidenced by 16S rRNA gene sequencing. C. sporogenes was not found. The same antibiotic treatment was continued. The evolution was favorable, with increasing features of bone union on X-ray images and no recurrence of infection. The recovery of the strain AIP 321.05 in six of the operative specimens at a 2-month interval led us to conclude that this bacterium was the cause of the chronicity of the infection for this patient.Strain AIP 321.05 was characterized by routine biochemical tests (10). Metabolic end products were assayed by quantitative gas chromatography as described previously (3). The bacterium was lecithinase and lipase negative on egg yolk agar plates. Gelatin and milk were not modified. Production of catalase and indole was not detected. Sulfite was reduced but not nitrate. Desulfoviridin was not produced. In Trypticaseglucose-yeast extract broth abundant gas was produced. The major metabolic end products were acetic acid, propionic acid, and butyric acid. Acid was produced from cellobiose, galactose, glucose, fructose, maltos...

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Soehngenia saccharolytica gen. nov., sp. nov. and Clostridium amygdalinum sp. nov., two novel anaerobic, benzaldehyde-converting bacteria

Cited by 84 publications

References 41 publications

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Desulfotomaculum carboxydivorans sp. nov., a novel sulfate-reducing bacterium capable of growth at 100 % CO

First Isolation of Clostridium amygdalinum from a Patient with Chronic Osteitis

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