“…In contrast, ultrasound is an attractive modality to manipulate micro-objects with distinct advantages of biocompatibility, deeper penetration, no requirement for special sample properties (conductivity, transparency, and more), and the ability to generate large forces. [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38] Currently, ultrasounddriven manipulation, in terms of trapping and rotating, has been achieved for microbeads, [39][40][41][42][43] cells, [44][45][46][47][48][49][50] and plant seeds, 51,52 by using surface acoustic waves and microbubbles. However, most of these subjects have nearly spherical structures with good symmetry and are ∼5-100 μm in size; only a few work to date has been conducted on model organisms with slender bodies like C. elegans (∼600-1000 μm) 53,54 and zebrafish (∼1-5 mm).…”