Soft‐Nanoparticle Functionalization of Natural Hydrogels for Tissue Engineering Applications

Elkhoury, Kamil; Russell, Carina S.; Sánchez-González, Laura; Mostafavi, Azadeh; Williams, Tyrell J.; Kahn, Cyril J.F.; Peppas, Nicholas A.; Arab‐Tehrany, Elmira; Tamayol, Ali

doi:10.1002/adhm.201900506

Cited by 110 publications

(80 citation statements)

References 191 publications

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“…4 These activities have mainly centered on the understanding and regulating of the interactions of cells, biomaterials, and biological factors. 5 Scaffolds have become a central platform for achieving the desired interactions between various cells and substrates. [1][2][3] As a result, various technologies such as electrospinning, micromolding, biotextiles, and three-dimensional (3D) printing (additive manufacturing) have been developed for the fabrication of scaffolds with biomimetic physicochemical properties and architectural features.…”

Section: Introductionmentioning

confidence: 99%

Process–Structure–Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds

Gerdes

Mostafavi

Ramesh

et al. 2020

Tissue Engineering Part A

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Bone defects are common and, in many cases, challenging to treat. Tissue engineering is an interdisciplinary approach with promising potential for treating bone defects. Within tissue engineering, three-dimensional (3D) printing strategies have emerged as potent tools for scaffold fabrication. However, reproducibility and quality control are critical aspects limiting the translation of 3D printed scaffolds to clinical use, which remain to be addressed. To elucidate the factors that yield to the generation of defects in bioprinting and to achieve reproducible biomaterial printing, the objective of this article is to frame a systematic approach for optimizing and validating 3D printing of poly(caprolactone) (PCL)-hydroxyapatite (HAp) composite scaffolds. We delineate the effect of PCL-to-HAp ratio, print velocity, print temperature, and extrusion pressure on the architectural and mechanical properties of the 3D printed scaffold. Furthermore, we present an in situ image-based monitoring approach to quantify key quality-related aspects of constructs, such as the ability to deposit material consistently and print elementary shapes with fewer flaws. Our results show that small defects generated during the printing process have a significant role in lowering the mechanical properties of 3D printed polymeric scaffolds. In addition, the in vitro osteoinductivity of the fabricated scaffolds is demonstrated.

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Section: Introductionmentioning

confidence: 99%

Process–Structure–Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds

Gerdes

Mostafavi

Ramesh

et al. 2020

Tissue Engineering Part A

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“…Liposomes are some of the oldest delivery carriers that are widely used to deliver bioactive agents to cells and tissues while protecting them from physiological barriers [13,14]. When encapsulated in liposomes, bioactive agents can be protected from stomach digestion and absorbed in substantial amounts in the gastrointestinal tract [15].…”

Section: Introductionmentioning

confidence: 99%

Growth-Inhibitory Effect of Chitosan-Coated Liposomes Encapsulating Curcumin on MCF-7 Breast Cancer Cells

et al. 2020

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Current anticancer drugs exhibit limited efficacy and initiate severe side effects. As such, identifying bioactive anticancer agents that can surpass these limitations is a necessity. One such agent, curcumin, is a polyphenolic compound derived from turmeric, and has been widely investigated for its potential anti-inflammatory and anticancer effects over the last 40 years. However, the poor bioavailability of curcumin, caused by its low absorption, limits its clinical use. In order to solve this issue, in this study, curcumin was encapsulated in chitosan-coated nanoliposomes derived from three natural lecithin sources. Liposomal formulations were all in the nanometric scale (around 120 nm) and negatively charged (around −40 mV). Among the three lecithins, salmon lecithin presented the highest growth-inhibitory effect on MCF-7 cells (two times lower growth than the control group for 12 µM of curcumin and four times lower for 20 µM of curcumin). The soya and rapeseed lecithins showed a similar growth-inhibitory effect on the tumor cells. Moreover, coating nanoliposomes with chitosan enabled a higher loading efficiency of curcumin (88% for coated liposomes compared to 65% for the non-coated liposomes) and a stronger growth-inhibitory effect on MCF-7 breast cancer cells.

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“…Liposomes are nano-scale systems widely used for the encapsulation, delivery, and controlled release of biologically active agents [ 15 , 16 , 17 ]. The unique architecture of liposomes allows the loading of hydrophobic and hydrophilic therapeutic molecules; their charge and surface properties can be tuned to enable stability during storage, control over the drug-release rate, and to meet specific therapeutic needs [ 18 , 19 ]. All these features allow liposomal nanoformulations to improve the drug therapeutic index and decrease its side effects [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Arab‐Tehrany

Elkhoury

Francius

et al. 2020

IJMS

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Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor ‎‎bioavailability in the human body, caused by its low aqueous solubility and fast degradation, ‎‎presents a big hurdle for its oral administration. Here, we used nano-vesicles made of ‎‎phospholipids to carry and protect curcumin in its membrane. Various curcumin amounts were ‎‎encapsulated in the produced phospholipid system to form drug-loaded liposomes. ‎Curcumin’s ‎concentration was evaluated using UV-visible measurements. The maximal ‎amount of curcumin ‎that could be added to liposomes was assessed. Nuclear magnetic ‎resonance (NMR) analyses ‎were used to determine curcumin’s interactions and localization ‎within the phospholipid ‎membrane of the liposomes. X-ray scattering (SAXS) and atomic ‎force microscopy (AFM) ‎experiments were performed to characterize the membrane structure ‎and organization, as well as its ‎mechanical properties at the nanoscale. Conservation of the membrane’s properties is found with ‎the addition of curcumin in various ‎amounts before saturation, allowing the preparation of a ‎defined nanocarrier with desired ‎amounts of the drug.

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Soft‐Nanoparticle Functionalization of Natural Hydrogels for Tissue Engineering Applications

Cited by 110 publications

References 191 publications

Process–Structure–Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds

Process–Structure–Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds

Growth-Inhibitory Effect of Chitosan-Coated Liposomes Encapsulating Curcumin on MCF-7 Breast Cancer Cells

Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

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