Soft-Tissue Imaging in a Human Mummy: Propagation-based Phase-Contrast CT

Romell, Jenny; Vågberg, William; Romell, Mikael; Häggman, Sofia; Ikram, Salima; Hertz, Hans M.

doi:10.1148/radiol.2018180945

Cited by 24 publications

(13 citation statements)

References 28 publications

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“…Must the subject be "reconstructed" after the autopsy for exposition?, etc. And, very importantly, the possibility of non-invasive techniques such as conventional radiology, computerized tomography or even magnetic resonance studies [18,19,20] should be considered, although the latter is not the best study technique in dried specimens. In some instances, the use of minimally invasive procedures such as endoscopy using a flexible cystoscope or a flexible fibrogastroscope can provide very useful images and samples [21,22].…”

Section: Methods Used In Paleopathologymentioning

confidence: 99%

Paleopathology of soft tissues: what mummies can reveal

Fernández

Esteban

Franco

2019

pjp

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Section: Methods Used In Paleopathologymentioning

confidence: 99%

Paleopathology of soft tissues: what mummies can reveal

Fernández

Esteban

Franco

2019

pjp

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“…Arranging two beam times for such experiments may represent a difficulty, but the use of cryo-cooled samples makes it possible to separate the imaging and MX datacollection steps in time, and ensure that the crystals do not move between these two steps and that registration is not lost. Alternatively, modern laboratorybased X-ray sources with small photonsource sizes and therefore sufficient spatial coherence for edge enhancement could be used, as reported in the literature for a variety of imaging methods (Bidola et al, 2017;Hauser et al, 2014;Mü ller et al, 2017;Romell et al, 2018;Zhou et al, 2015). One could, in principle, think of installing such a dedicated X-ray source either next to or at an MX beamline, or use laboratory equipment.…”

Section: Prelocation By 2d Full-field X-ray Imagingmentioning

confidence: 99%

Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography

Martiel

Huang

Villanueva-Pérez

et al. 2020

Int Union Crystallogr J

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Serial protein crystallography has emerged as a powerful method of data collection on small crystals from challenging targets, such as membrane proteins. Multiple microcrystals need to be located on large and often flat mounts while exposing them to an X-ray dose that is as low as possible. A crystal-prelocation method is demonstrated here using low-dose 2D full-field propagation-based X-ray phase-contrast imaging at the X-ray imaging beamline TOMCAT at the Swiss Light Source (SLS). This imaging step provides microcrystal coordinates for automated serial data collection at a microfocus macromolecular crystallography beamline on samples with an essentially flat geometry. This prelocation method was applied to microcrystals of a soluble protein and a membrane protein, grown in a commonly used double-sandwich in situ crystallization plate. The inner sandwiches of thin plastic film enclosing the microcrystals in lipid cubic phase were flash cooled and imaged at TOMCAT. Based on the obtained crystal coordinates, both still and rotation wedge serial data were collected automatically at the SLS PXI beamline, yielding in both cases a high indexing rate. This workflow can be easily implemented at many synchrotron facilities using existing equipment, or potentially integrated as an online technique in the next-generation macromolecular crystallography beamline, and thus benefit a number of dose-sensitive challenging protein targets.

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“…The x‐ray source needs to provide radiation with enough spatial coherence to enable interference, and therefore this type of imaging has mainly been performed at synchrotron facilities 26 . Recent developments in electron‐impact x‐ray sources have enabled laboratory phase‐contrast imaging using PBI, with promising results in, for example, zebrafish muscle, 27 mouse brain, 28 velvet worm myoanatomy 29 and mummified soft tissue 30 . A few cases of phase‐contrast imaging of insects have been reported, but they are to date limited to fossils in amber and stained or dried specimens (see, for example, Refs 31,32 …”

Section: Introductionmentioning

confidence: 99%

Laboratory phase‐contrast nanotomography of unstained Bombus terrestris compound eyes

Romell

Jie

Miettinen

et al. 2021

Journal of Microscopy

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Imaging the visual systems of bumblebees and other pollinating insects may increase understanding of their dependence on specific habitats and how they will be affected by climate change. Current high‐resolution imaging methods are either limited to two dimensions (light‐ and electron microscopy) or have limited access (synchrotron radiation x‐ray tomography). For x‐ray imaging, heavy metal stains are often used to increase contrast. Here, we present micron‐resolution imaging of compound eyes of buff‐tailed bumblebees (Bombus terrestris) using a table‐top x‐ray nanotomography (nano‐CT) system. By propagation‐based phase‐contrast imaging, the use of stains was avoided and the microanatomy could more accurately be reconstructed than in samples stained with phosphotungstic acid or osmium tetroxide. The findings in the nano‐CT images of the compound eye were confirmed by comparisons with light‐ and transmission electron microscopy of the same sample and finally, comparisons to synchrotron radiation tomography as well as to a commercial micro‐CT system were done.

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Soft-Tissue Imaging in a Human Mummy: Propagation-based Phase-Contrast CT

Cited by 24 publications

References 28 publications

Paleopathology of soft tissues: what mummies can reveal

Paleopathology of soft tissues: what mummies can reveal

Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography

Laboratory phase‐contrast nanotomography of unstained Bombus terrestris compound eyes

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