Soft wheat cultivars grown in the Saratov region and their resistance to Septoria blotch

Zeleneva, Yu. V.; Konkova, E. A.

doi:10.18699/vjgb-23-70

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…The wheat genotypes from Kazakhstan, Russia, and CIMMYT differed greatly in Septoria severity recorded in the adult plant stage in the field in Kazakhstan. This supports reports on varietal resistance and variation among Z. tritici populations in Kazakhstan and Russia [ 4 , 33 , 41 , 42 , 43 , 44 , 45 , 46 ]. Zeleneva and Konkova (2023a) evaluated the resistance of winter and spring wheat cultivars to detect resistance to Septoria blotch and studied the populations of Parastagonospora nodorum and P. pseudonodorum in the territory of the Saratov region of Russia to detect the presence of effector genes; eleven wheat genotypes were selected as resistant to different species of Septoria blotch ( Zymoseptoria tritici , P. nodorum , P. pseudonodorum ) [ 41 ].…”

Section: Discussionsupporting

confidence: 91%

Identification of Wheat Septoria tritici Resistance Genes in Wheat Germplasm Using Molecular Markers

Kokhmetova,

Bolatbekova,

Zeleneva

et al. 2024

Plants

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Zymoseptoria tritici (Z. tritici) is the main threat to global food security; it is a fungal disease that presents one of the most serious threats to wheat crops, causing severe yield losses worldwide, including in Kazakhstan. The pathogen leads to crop losses reaching from 15 to 50%. The objectives of this study were to (1) evaluate a wheat collection for Z. tritici resistance during the adult plant and seedling growth stages, (2) identify the sources of resistance genes that provide resistance to Z. tritici using molecular markers linked to Stb genes, and (3) identify potentially useful resistant wheat genotypes among cultivars and advanced breeding lines. This study evaluated 60 winter and spring wheat genotypes for Z. tritici resistance. According to the field reactions, 22 entries (35.7%) showed ≤10% disease severity in both years. The resistant reaction to a mix of Z. tritici isolates in the seedling stage was associated with adult plant resistance to disease in four wheat entries. The resistance of Rosinka 3 was due to the presence of Stb8; Omskaya 18 showed an immune reaction in the field and a moderately susceptible reaction in the seedling stage, possibly provided by a combination of the Stb7 and Stb2 genes. The high resistance in both the adult and seedling stages of Omskaya 29 and KR11-03 was due to the Stb4 and Stb2 genes and, possibly, due to the presence of unknown genes. A linked marker analysis revealed the presence of several Stb genes. The proportion of wheat entries with Stb genes was quite high at twenty-seven of the genotypes tested (45.0%), including four from Kazakhstan, nine from Russia, nine from the CIMMYT-ICARDA-IWWIP program, and five from the CIMMYT-SEPTMON nursery. Among the sixty entries, ten (16.7%) carried the resistance genes Stb2 and Stb8, and the gene Stb4 was found in seven cultivars (11.6%). Marker-assisted selection can be efficiently applied to develop wheat cultivars with effective Stb gene combinations that would directly assist in developing durable resistance in Kazakhstan. Resistant genotypes could also be used as improved parents in crossing programs to develop new wheat cultivars.

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Section: Discussionsupporting

confidence: 91%

Identification of Wheat Septoria tritici Resistance Genes in Wheat Germplasm Using Molecular Markers

Kokhmetova,

Bolatbekova,

Zeleneva

et al. 2024

Plants

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Characteristics of Zymoseptoria tritici isolates from the Russian Federation and the Republic of Kazakhstan by azoxystrobin sensitivity

Zeleneva,

Zubko,

Kokhmetova

et al. 2024

BIO Web Conf.

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Septoria tritici blotch is one of the harmful diseases of wheat cultivars cultivated in the Russian Federation and the Republic of Kazakhstan. The purpose of the research was to study a sample of Zymoseptoria tritici isolates obtained from the Saratov and Tambov (Russian Federation) and Almaty (Republic of Kazakhstan) regions according to the degree of the azoxystrobin sensitivity, as well as to evaluate these isolates for the presence of the G143A mutation using PCR-RFLP analysis. The azoxystrobin sensitivity of Z. tritici isolates was evaluated by studying the growth restriction of cultures on a nutrient medium (concentrations of active drug in the medium were 100, 10, 1, 0.1, 0.01 mg/l.). In the control, on the 21st day of cultivation, the diameter of the colonies of Z. tritici strains varied from 14.24±1.43 mm to 19.53±0.66 mm. Azoxystrobin had a significant inhibitory effect on the growth of fungal isolates from the Tambov region: EC50 is 1.72 μg/l, from the Almaty region EC50 is 2.36 μg/l, from the Saratov region EC50 is 2.63 μg/l. The results of biotesting made it possible to identify isolates of Z. tritici resistant to azoxystrobin: 277-22-5, 277-22-13 (from Kazakhstan); 104-23-9, 104-23-10 (from the Saratov region); 19-23-5, 19-23-8 (from the Tambov region). PCR-RFLP method made it possible to identify mitochondrial mutations G143A in selected fungal isolates, which are associated with the development of resistance to fungicides in the causative agent of septoria. This work is the beginning of a more in-depth study of the sensitivity of the economically significant phytopathogen Z. tritici to fungicides in the territories of Russia and the Republic of Kazakhstan.

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Identification of effector genes of <i>Parastagonospora nodorum</i>, <i>P. pseudonodorum</i> in Tambov populations and sensitivity genes to NEs in varieties and hybrid lines of spring soft wheat

Zeleneva,

Sudnikova,

Gusev

et al. 2024

Ecological genetics

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BACKGROUND: The purpose of the work is to assess the populations of the fungi Parastagonospora nodorum and P. pseudonodorum in 2023 based on the presence of effector genes, as well as to identify alleles of Snn1/snn1, Snn3/snn3 genes that control the sensitivity or resistance of wheat to PtrTox1 and PtrTox3 toxins. MATERIALS AND METHODS: Infectious samples were collected in 2023 from spring wheat leaves. In addition, the material for the study was 2 varieties and 23 lines of spring soft wheat of local selection. Using the molecular markers Xfcp624 and Xcfd20, the presence of the Snn1 and Snn3-B1 alleles, which control sensitivity to the fungal toxins PtrTox1 and PtrTox3, was detected. RESULTS: Using molecular screening, ToxA and Tox1 genes were identified in genotypes of P. pseudonodorum isolates; Tox3 and Tox267 in P. nodorum isolates. 2 varieties of spring soft wheat and 11 hybrid lines carry a recessive allele snn1, which protects against the phytopathogen toxin PtrTox1; wheat variety Tambovchanka and 2 hybrid lines (Stb-7/15, Rl-6-22) carry the recessive allele snn3 on chromosome B1, which confers resistance to the fungal toxin PtrTox3. CONCLUSIONS: The ToxA gene was found only among monoconidial isolates of P. pseudonodorum species obtained from leaves of spring soft wheat of Lebedushka variety. As a result of molecular screening, the Tox1 gene was identified among 70 P. pseudonodorum isolates. The presence of the Tox3 and Tox267 genes was established in 30 isolates of P. nodorum species obtained from plant samples of spring durum wheat Donskaya Elegiya.The variety of Pamyati Plakhotnika and 11 hybrid lines carry one recessive allele tsn1, snn1 or snn3, protecting the plant at the genetic level from the toxins PtrToxA, PtrTox1 and PtrTox3, respectively. Variety Tambovchanka and 7 lines have protection against two toxins at once.

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Soft wheat cultivars grown in the Saratov region and their resistance to Septoria blotch

Cited by 4 publications

References 28 publications

Identification of Wheat Septoria tritici Resistance Genes in Wheat Germplasm Using Molecular Markers

Identification of Wheat Septoria tritici Resistance Genes in Wheat Germplasm Using Molecular Markers

Characteristics of Zymoseptoria tritici isolates from the Russian Federation and the Republic of Kazakhstan by azoxystrobin sensitivity

Identification of effector genes of <i>Parastagonospora nodorum</i>, <i>P. pseudonodorum</i> in Tambov populations and sensitivity genes to NEs in varieties and hybrid lines of spring soft wheat

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