Abstract:Scanning transmission X-ray microscopy (STXM) provides the imaging of biological specimens allowing the parallel collection of localized spectroscopic information by X-ray fluorescence (XRF) and/or X-ray Absorption Near Edge Spectroscopy (XANES). The complex metabolic mechanisms which can take place in biological systems can be explored by these techniques by tracing even small quantities of the chemical elements involved in the metabolic pathways. Here, we present a review of the most recent publications in t… Show more
“…Bonanni and Gianoncelli 28 presented a comprehensive review of advancements in the soft X-ray regime for studying metabolic features in biological systems. The paper discussed the combined use of low-energy XRF spectrometry, STXM and XANES techniques and provided valuable information on synchrotron soft X-ray microspectroscopy beamlines suitable for metabolic research.…”
Section: Synchrotron and Large-scale Facilitiesmentioning
This review covers developments in and applications of XRF techniques such as EDXRF, WDXRF, TXRF, XRF microscopy using technologies such as synchrotron sources, X-ray optics, X-ray tubes and detectors in laboratory, mobile and hand-held systems.
“…Bonanni and Gianoncelli 28 presented a comprehensive review of advancements in the soft X-ray regime for studying metabolic features in biological systems. The paper discussed the combined use of low-energy XRF spectrometry, STXM and XANES techniques and provided valuable information on synchrotron soft X-ray microspectroscopy beamlines suitable for metabolic research.…”
Section: Synchrotron and Large-scale Facilitiesmentioning
This review covers developments in and applications of XRF techniques such as EDXRF, WDXRF, TXRF, XRF microscopy using technologies such as synchrotron sources, X-ray optics, X-ray tubes and detectors in laboratory, mobile and hand-held systems.
“…14 Soft X-ray microscopy is indeed becoming a more and more widespread analytical tool for investigating complex systems at the micrometric and sub-micrometric levels, especially when combined with X-ray spectroscopy capabilities. 15 Several synchrotrons worldwide have installed STXM beamlines, [16][17][18][19][20][21][22][23][24][25] similar to each other but at the same time each with unique capabilities. STXM is a scanning technique where the sample is usually raster-scanned across a micro-or nano-probe by photons delivered by suitable X-ray optics (Fig.…”
The development of an in situ combination of AFM and soft X-ray μXRF in a single instrument, with AFM integration accomplished without substantial original set-up alteration.
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