“…Soft X-ray (SXR) microscopy has been successfully employed mainly in transmission mode, either using diffractive optics, such as zone-plates [23][24][25], raster scanning of the sample by focused SXR beam [26][27][28] or as a contact microscopy, where the sample is placed on top of a recording medium, such as a photoresist, and illuminated by SXR beam to make a ''picture'' of the specimen in the surface of the recording medium [29][30][31]. Synchrotron radiation at k = 2.4 nm was used for imaging frozenhydrated samples at atmospheric pressure, where details inside cells of algae as small as 35 nm were visible [32], or to examine rapidly frozen mouse 3T3 cells and obtained excellent cellular morphology at better than 50 nm lateral resolution, using transmission SXR microscope [33].…”