The effects of Zinc (Zn) on lipid peroxidation, antioxidative enzymes, growth, Zn accumulation, and leaf chlorophyll of Phyllostachys pubescens (Pradelle) Mazel ex J.Houz. were investigated in two greenhouse experiments. Hydroponics experiment with Zn application of 0, 20, 100, and 400 μM revealed that lower concentration of Zn in solution led to increased malondialdehyde (MDA) and proline contents but inhibited SOD activity in all treatments. P. pubescens had showed strong ability to accumulate Zn in stems and reached maximum level at 100 μM with 7.91-fold increase compared with control. In pot experiment, treatment with Zn ranged from 0, 200, 400, 800, 1,600, to 3,200 mg kg(-1). Application of 800 mg kg(-1) revealed 116, 24.6, and 28.3 times increase in Zn concentration of roots, stems, and leaves, respectively. Growth and chlorophyll contents of plants in pots were better promoted at 400 mg kg(-1) Zn, with 60.5 and 30.9 % enhanced roots and shoot compared with control. The bioaccumulation factor (BAF) was in the sequence of stem > roots > leaves. The translocation factor (TF) of stem was higher than leaves.