Soil Collected in the Great Smoky Mountains National Park Yielded a Novel
            <i>Listeria sensu stricto</i>
            Species,
            <i>L. swaminathanii</i>

Carlin, Catharine R; Liao, Jingqiu; Hudson, Lauren K.; Peters, Tracey L.; Denes, Thomas G.; Orsi, Renato H.; Guo, Xiaodong; Wiedmann, Martin

doi:10.1128/spectrum.00442-22

Cited by 18 publications

(15 citation statements)

References 59 publications

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“…'L. swaminathanii' is a novel sensu stricto species that was originally and subsequently isolated from the Great Smoky Mountains National Park [2,22]. That, along with the isolation of this species in the present study, provides support that 'L.…”

Section: Discussionsupporting

confidence: 73%

“…Most of the distinct strains (13 of 14) isolated in this study are in the sensu stricto clade, except for the single L. booriae isolate. The Listeria sensu stricto clade currently contains the pathogenic species L. monocytogenes and L. ivanovii, along with eight other species [5,22,24]. In food processing environments, the detection of sensu stricto Listeria species implies the potential presence of L. monocytogenes [2,22].…”

Section: Discussionmentioning

confidence: 99%

“…The Listeria sensu stricto clade currently contains the pathogenic species L. monocytogenes and L. ivanovii, along with eight other species [5,22,24]. In food processing environments, the detection of sensu stricto Listeria species implies the potential presence of L. monocytogenes [2,22]. Nevertheless, further investigation of the co-presence of L. monocytogenes and other non-pathogenic sensu stricto clade species in natural environments is required to identify index strains that could indicate the presence of L. monocytogenes.…”

Section: Discussionmentioning

confidence: 99%

“…Nantahala National Forest (NNF), the largest national forest in North Carolina, covers over 531,000 acres with elevations ranging from 1,200 ft to 5,800 ft (366 to 1768 meters) [21]. Previous investigations have shown that there is a great deal of biodiversity of Listeria within national forests and national parks in the southern Appalachian region of the United States [2,3,22,23]. Previous research suggests that soil moisture, molybdenum, and salt concentrations signi cantly drive the presence or absence of Listeria spp.…”

Section: Introductionmentioning

confidence: 99%

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Whole-Genome Sequencing and Metagenomics Reveals Diversity and Prevalence of Soil Listeria spp. in the Nantahala National Forest

Wang,

Schamp,

Hudson

et al. 2023

Preprint

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Background. Listeria spp. are widely distributed environmental bacteria associated with human foodborne illness. Whole-genome sequencing (WGS) of Listeria monocytogenes clinical isolates is the current standard for surveillance and identifying outbreaks. WGS of Listeria isolates can assist in understanding the diversity of Listeria species. The capability of precise detection and characterization of Listeria spp. strains in the natural environment is important for identifying transmission routes that can lead to food contamination and human illness. Recently, metagenomic sequencing has emerged as a promising tool for the direct detection of Listeria spp. in environmental samples. The objective of this study was to use a practical hybrid approach that can provide valuable information on the occurrence of Listeria spp. in soil environments in the southern Appalachian region of the United States. Results. This study involved soil sampling from four locations across varying altitudes in the Nantahala National Forest. Forty-two Listeria spp. isolates and 12 metagenomes of soil bacterial communities were obtained. Analysis of the isolates genomes revealed 14 distinct strains, representing a diversity of Listeria species. The predominant species isolated was L. cossartiae subsp. cayugensis (n = 8), with the other species including L. monocytogenes (n = 3), ‘L. swaminathanii’ (n = 1), L. marthii (n = 1), and L. booriae (n = 1). Most distinct strains (n = 13) were isolated from lower altitudes (1,500 or 2,500 ft), while the ‘L. swaminathanii’ strain was isolated from two different altitudes. Metagenomic analysis demonstrated a decline in both bacterial community diversity and relative abundance of Listeria spp. with increasing altitude. Of the soil factors assessed, pH and cation exchange capacity were significantly positively correlated with the abundance of Listeria spp. in soil as detected by metagenomics. Conclusions. By integrating culture-independent metagenomics with culture-based WGS, this study provides insights into the diversity and prevalence of Listeria spp. in the Nantahala National Forest. These findings contribute to the current knowledge on the distribution of Listeria spp. in the natural environment and offer a potential culture-independent method for tracking the transmission of foodborne pathogens.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Whole-Genome Sequencing and Metagenomics Reveals Diversity and Prevalence of Soil Listeria spp. in the Nantahala National Forest

Wang,

Schamp,

Hudson

et al. 2023

Preprint

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“…type strains, suggesting they would qualify as a novel species. After the initial submission of this work, these novel species isolates were further characterized and determined to belong to the proposed novel species “ Listeria swaminathanii ” ( 34 , 35 ).…”

Section: Resultsmentioning

confidence: 99%

Soil Collected from a Single Great Smoky Mountains Trail Contains a Diversity of Listeria monocytogenes andListeriaspp.

et al. 2023

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Listeria monocytogenes is a foodborne pathogen that can cause serious systemic illness that, although rare, usually results in hospitalization and has a relatively high mortality rate compared to other foodborne pathogens. Identification of novel and diverse Listeria spp. can provide insights into the genomic evolution, ecology, and evolution and variance of pathogenicity of this genus, especially in natural environments.

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Phylogenomic analyses of the Listeriaceae family support species reclassification and proposal of a new family and new genera

Bouznada,

Belaouni,

Saker

et al. 2024

Antonie van Leeuwenhoek

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Soil Collected in the Great Smoky Mountains National Park Yielded a Novel Listeria sensu stricto Species, L. swaminathanii

Cited by 18 publications

References 59 publications

Whole-Genome Sequencing and Metagenomics Reveals Diversity and Prevalence of Soil Listeria spp. in the Nantahala National Forest

Whole-Genome Sequencing and Metagenomics Reveals Diversity and Prevalence of Soil Listeria spp. in the Nantahala National Forest

Soil Collected from a Single Great Smoky Mountains Trail Contains a Diversity of Listeria monocytogenes andListeriaspp.

Phylogenomic analyses of the Listeriaceae family support species reclassification and proposal of a new family and new genera

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