Soil metabolomics: Deciphering underground metabolic webs in terrestrial ecosystems

Song, Yang; Yao, Shi; Li, Xiaona; Wang, Tao; Jiang, Xin; Bolan, Nanthi; Warren, Charles R.; Northen, Trent R.; Chang, Scott X.

doi:10.1016/j.eehl.2024.03.001

Cited by 2 publications

(2 citation statements)

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“…LDI-FT-ICR-MS allowed for a direct view into the bandwidth of molecules that constitute SOM, and provided deeper insights into the molecular effects of long-term FYM addition. The technique may therefore complement existing targeted and non-targeted LC-MS approaches (Bahureksa et al, 2021;Brown et al, 2024;Song et al, 2024). Of special interest is the ability to study both soluble (e.g., as powder from dried extracts or via direct comparison with liquid-state FT-ICR-MS) and non-soluble compounds (soil particles, soil fractions, etc.…”

Section: Limitations Of the Approachmentioning

confidence: 99%

“…) or individual biomarker level (lignin phenols, amino sugars, etc.). Few have employed non-targeted metabolomics approaches having the potential to elucidating SOM formation, turnover, and stabilization on a molecular level (Li et al, 2023;Brown et al, 2024;Song et al, 2024). Tang et al (2023) used liquid chromatography coupled to time of flight tandem mass spectrometry (LC-MS) and found that extractable SOM (using organic solvents) responded to long-term FYM addition.…”

Section: Introductionmentioning

confidence: 99%

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Long-term effects of farmyard manure addition on soil organic matter molecular composition: C transformation as a major driver of energetic potential

Simon,

Miltner,

Mulder

et al. 2024

Preprint

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Long-term addition of farmyard manure (FYM) supports the accumulation of microbial C and soil organic matter (SOM), but the effects on energy storage remain unknown. In particular, it remains unresolved whether FYM or the stimulation of microbial transformations explains the increased microbial imprint. The latter would suggest that the accumulation of SOM transformation products controls energy storage, rather than FYM directly. We hypothesized that the overlap with original FYM signatures could be used as a measure of SOM transformation and its effect on SOM's nominal oxidation state of C (NOSC) and energetic potential ΔG0Cox. We employed solid-state laser desorption ionization Fourier transform mass spectrometry (LDI-FT-ICR-MS) to study molecular signatures of FYM samples and topsoil from four long-term field experiments receiving FYM, and unfertilized controls. In line with bulk elemental analysis, LDI-FT-ICR-MS suggested that FYM addition increased SOM's energetic potential (0.7 – 1.2 kJ/mol C). FYM addition changed SOM composition by 3 - 16% of ion abundance as compared to controls, being larger in longer-running field experiments. Markers unrelated to original FYM signatures (i.e., indirect effects) explained 67 – 84% of molecular changes while markers directly related to FYM explained only 2 – 12%. FYM addition shifted molecular composition to higher H/C, O/C and m/z, and lower aromaticity. Accumulated molecules had higher energetic potentials and were, despite being chemically similar to original FYM, elevated in mass, suggesting potential use of FYM-derived building blocks for microbial synthesis of larger molecules. Vanishing molecules showed lower energetic potential, mirroring the higher oxidation state of water-extractable organic matter, which pointed to an increased solubility of SOM. Our results indicate a uniform shift in SOM properties upon FYM addition, but highlight the role of site-specific trajectories of SOM compositional change. We discuss the implications of FYM-induced microbial transformations for energy storage and long-term stability of SOM.

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Section: Limitations Of the Approachmentioning

confidence: 99%