Soil metagenomics reveals differences under conventional and no-tillage with crop rotation or succession

Souza, Renata Carolini; Cantão, Maurício Egídio; Vasconcelos, Ana Tereza Ribeiro de; Nogueira, Marco Antônio; Hungría, Mariangela

doi:10.1016/j.apsoil.2013.05.021

Cited by 109 publications

(114 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is well known that fungi have optimal growth in the 2.0 to 7.0 pH range, whereas, for bacteria, the best range is between pH 5.0 and 9.0 (SMITH and DORAN, 1996). Our results are also consistent with a metagenomic analysis in a similar area at the same experimental station, showing greater abundance of fungi with CT than with NT, and the authors suggested that it may be related to higher tolerance of fungi to environmental stresses (SOUZA et al, 2013).…”

Section: Resultssupporting

confidence: 81%

Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil

Babujia¹,

Silva²,

Nogueira³

et al. 2014

Rev. Ciênc. Agron.

Self Cite

View full text Add to dashboard Cite

-The no-tillage (NT) system of soil management is recognized as more sustainable than conventional tillage (CT), with an important role played by soil microorganisms. The objective of this study was to estimate differences in soil microbial diversity under NT and CT at different soil depths. For that, bacterial (16S rDNA) and fungal (18S rDNA) communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in a 20-year field experiment established on an Oxisol in southern Brazil on which soybean has been grown in the summer and wheat in the winter. Soil samples were collected at the depths of 0-5, 5-10, 10-20 and 20-30 cm, and submitted to DGGE analyses. The results revealed lower similarity (28%) between bacterial communities in the NT and the CT systems at the 0-5 cm layer. The Shannon index (H) confirmed higher bacterial diversity with NT at all depths, when compared to CT. In relation to fungal communities, higher diversity was detected with CT, especially at the 0-5-cm depth. The results indicate that fungal communities can be more tolerant to environmental stresses related to soil disturbance than bacteria. More emphasis should be given for understanding processes affecting the diversity of microorganisms in agricultural soils, with particular emphasis on tillage systems.Key words: Bacterial diversity. Fungal diversity. PCR-DGGE. Soil tillage.RESUMO -O sistema de plantio direto (PD) é reconhecido como mais sustentável que o plantio convencional (PC), desempenhando um importante papel na atividade dos microrganismos do solo. O objetivo deste estudo foi estimar diferenças na diversidade microbiana do solo sob PD e PC, em diferentes profundidades. Para isso, foram avaliadas as comunidades bacteriana (16S rDNA) e fúngica (18S rDNA) através da eletroforese em gel com gradiente desnaturante (DGGE), em um experimento de campo estabelecido há 20 anos em um Latossolo Vermelho no sul do Brasil, com a soja cultivada no verão e trigo no inverno. As amostras de solo foram coletadas nas profundidades de 0-5; 5-10; 10-20 e 20-30 cm, e foram submetidas a análises de DGGE. Os resultados revelaram baixa similaridade (28%) na profundidade de 0-5 cm para as comunidades bacterianas entre o sistema de PD e PC. O índice de Shannon (H) confirmou diversidade bacteriana superior no PD em todas as profundidades, quando comparado ao PC. Em relação às comunidades fúngicas, a maior diversidade foi detectada no sistema de PC, especialmente na profundidade de 0-5 cm. Os resultados indicam que as comunidades fúngicas podem ser mais tolerantes a estresses ambientais relacionados com a perturbação do solo que as bactérias. Maior ênfase deve ser dada para compreender os processos que afetam a diversidade dos microrganismos em solos agrícolas, com particular destaque para os sistemas de preparo do solo. Palavras-chave: Diversidade bacteriana. Diversidade fúngica. PCR-DGGE. Manejo do Solo

show abstract

Section: Resultssupporting

confidence: 81%

Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil

Babujia¹,

Silva²,

Nogueira³

et al. 2014

Rev. Ciênc. Agron.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The long-term cultivation might affect the whole soil community. Souza et al (2013) showed that the major differences among the soil biodiversity and microbial community structures were associated with tillage management. Differences among the soil structures induced by different tillage systems and land-use conversion influence the structures of microbial communities (Sessitsch et al, 2001;Peixoto et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Land-use changes influence soil bacterial communities in a meadow grassland in Northeast China

et al. 2017

View full text Add to dashboard Cite

Abstract. The conversion of natural grassland into agricultural fields is an intensive anthropogenic perturbation commonly occurring in semiarid regions, and this perturbation strongly affects soil microbiota. In this study, the influences of land-use conversion on the soil properties and bacterial communities in the Horqin Grasslands in Northeast China were assessed. This study aimed to investigate (1) how the abundances of soil bacteria changed across land-use types, (2) how the structure of the soil bacterial community was altered in each land-use type, and (3) how these variations were correlated with soil physical and chemical properties. Variations in the diversities and compositions of bacterial communities and the relative abundances of dominant taxa were detected in four distinct land-use systems, namely, natural meadow grassland, paddy field, upland field, and poplar plantation, through the high-throughput Illumina MiSeq sequencing technique. The results indicated that landuse changes primarily affected the soil physical and chemical properties and bacterial community structure. Soil properties, namely, organic matter, pH, total N, total P, available N and P, and microbial biomass C, N, and P, influenced the bacterial community structure. The dominant phyla and genera were almost the same among the land-use types, but their relative abundances were significantly different. The effects of land-use changes on the structure of soil bacterial communities were more quantitative than qualitative.

show abstract

“…Although the fungi to bacteria dominance under NT system is broadly accepted by the scientific community, Strickland and Rousk (2010) have suggested research addressing the mechanism by which the eventual shift may occur, because recent studies have shown that NT enhances both fungal and bacterial biomass. For example, a metagenomic study comparing NT and CT in a similar soil of Londrina, the dominance of bacteria in CT was not confirmed (Souza et al, 2013). Independent of shifts in the fungi-to-bacteria ratio, the changes in the microbial biomass C/N ratio may also affect the rate of immobilization and mineralization of soil nutrients, as more nutrients are stored in microbial biomass.…”

Section: Discussionmentioning

confidence: 99%

Soil Quality in Relation to Forest Conversion to Perennial or Annual Cropping in Southern Brazil

Balota

Yada

Amaral

et al. 2015

Rev. Bras. Ciênc. Solo

Self Cite

View full text Add to dashboard Cite

Many forested areas have been converted to intensive agricultural use to satisfy food, fiber, and forage production for a growing world population. There is great interest in evaluating forest conversion to cultivated land because this conversion adversely affects several soil properties. We examined soil microbial, physical, and chemical properties in an Oxisol (Latossolo Vermelho distrófico) of southern Brazil 24 years after forest conversion to a perennial crop with coffee or annual grain crops (maize and soybeans) in conventional tillage or no-tillage. One goal was to determine which soil quality parameters seemed most sensitive to change. A second goal was to test the hypothesis that no-tillage optimized preservation of soil quality indicators in annual cropping systems on converted land. Land use significantly affected microbial biomass and its activity, C and n mineralization, and aggregate stability by depth. Cultivated sites had lower microbial biomass and mineralizable C and N than a forest used as control. The forest and no-tillage sites had higher microbial biomass and mineralizable C and N than the conventional tillage site, and the metabolic quotient was 65 and 43 % lower, respectively. Multivariate analysis of soil microbial properties showed a clear separation among treatments, displaying a gradient from conventional tillage to forest. Although the soil at the coffee site was less disturbed and had a high organic C content, the microbial activity was low, probably due to greater soil acidity and Al toxicity. Under annual cropping, microbial activity in no-tillage was double that of the conventional tillage management. The greater microbial activity in forest and no-tillage sites may be attributed,

show abstract

Soil metagenomics reveals differences under conventional and no-tillage with crop rotation or succession

Cited by 109 publications

References 79 publications

Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil

Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil

Land-use changes influence soil bacterial communities in a meadow grassland in Northeast China

Soil Quality in Relation to Forest Conversion to Perennial or Annual Cropping in Southern Brazil

Contact Info

Product

Resources

About