Soil microbial community characteristics under different vegetation types at the national nature reserve of Xiaolongshan Mountains, Northwest China

Yang

et al. 2022

Front. Environ. Sci.

Revegetation is an important restoration approach after reclamation in coal mining subsidence area. However, few studies have paid attention to the impacts of different vegetation species on soil fertility and bacterial community composition in the reclamation area filled with fly ash for a long time. In this study, soil fertility and bacterial community composition were investigated in a non-subsidence area (FCK) and a coal mining subsidence reclamation area restored vegetation with woody plants (pagoda trees, FS; peach trees, FP) and herbaceous plants (wheat-maize rotation, FW) for 20 years. Results showed that topsoil and subsoil nutrients including available K and alkali-hydrolyzable N, soil organic matter, and total carbon in the non-subsidence site were significantly higher than those in reclamation sites. Topsoil fertility indices (SFI) in reclamation sites were lower than that in non-subsidence site, and soil fertility index in FW was higher than that in FS and FP. Moreover, the SFI in topsoil (from −0.24 to 2.08) was significantly higher than that in subsoil (from −1.12 to −0.39). 16S rRNA gene sequencing analysis showed Proteobacteria, Acidobacteria and Actinobacteria were the dominant bacterial phyla in all sites, but the subsoil bacterial alpha indices (Shannon and Simpson) in the non-subsidence site were higher than those in reclamation sites. Principal coordinates analysis and non-metric multidimensional scaling analysis showed that the bacterial community composition in reclamation sites was significantly different from that in the non-subsidence site, and they were similar in the soil with wheat-maize rotation model between non-subsidence and reclamation sites. Co-occurrence network analysis noted that the network of dominant bacterial operational taxonomic units in the subsoil was more complex than that in the topsoil. Redundancy analysis suggested soil pH in topsoil and subsoil was an important driving factor for soil bacteria community composition. Overall, the reclamation site with the wheat-maize rotation model had higher soil fertility and bacterial community composition was similar to that of the non-subsidence site, indicating revegetation with the wheat-maize rotation model is a good reclamation approach to improve soil fertility in coal mining subsidence area.

Section: Impacts Of Soil Physicochemical Properties On Soil Microbial...supporting

confidence: 55%

Response of Soil Fertility and Bacterial Community Composition to Vegetation Species in a Coal Mining Subsidence Area: A Survey After 20-Year Reclamation

Yang

et al. 2022

Front. Environ. Sci.

“…Actinomycetes exhibit hyphal growth patterns in soil, enabling plants to expand their surface area to deeper soil layers to take up nutrients; these mycelia form soil aggregates and act as active components for preserving water and nutrients. The dominant fungal phylum in soil is Ascomycota and Basidiomycota, which usually form symbionts with roots in dry and cold habitats ( 44 ). Most saprophytic fungi are Ascomycota that rapidly metabolize organic substrates in soils and which are more involved in cellulolysis compared to Basidiomycetes ( 45 ).…”

Section: Discussionmentioning

confidence: 99%

Compared with pure forest, mixed forest alters microbial diversity and increases the complexity of interdomain networks in arid areas

Guo,

Gong

2024

Microbiol Spectr

Soil microbial communities are key to material cycling and energy exchange in forest soils. As the Tianshan Mountains in Xinjiang are rich in tree species, it is important to understand the impact of tree species composition on soil microbial community structure and co-occurrence networks in arid mountain habitats. In this study, soil microbial community structure of five typical vegetation types in the Tianshan Mountains of Xinjiang, China, was investigated using bacterial 16S rRNA and fungal ITS sequences. The results showed that the relationship between the fungal community and tree species was strong, while the bacterial community was mainly affected by soil carbon. The dominant bacterial composition was Ascomycota, which had the highest relative abundance in herbaceous soils (27%–41%); the dominant fungal phylum was Ascomycota (50.22%–63.95% of the total sequences), followed by Stramonium (8.99%–28.09%). The fungal community structure of mixed coniferous and broadleaf forests was similar to that of conifers, while the bacterial community structure was similar to that of pure broadleaf forests. Mixed forests (mean connectivity: 812.50, clustering coefficient: 8.89) showed a more complex co-occurrence network and a significant increase in microbial structure related to soil carbon and nitrogen than pure forests (broad: mean connectivity: 710.69, clustering coefficient: 6.94; coniferous: mean connectivity: 730.79, clustering coefficient: 7.76). The results contribute to the understanding of the interactions between soil microorganisms and soil factors in different forest ecosystems and provide new insights into the interactions among forest microorganisms in arid zones. IMPORTANCE The results provide a comparative study of the response of soil microbial ecology to the afforestation of different tree species and deepen the understanding of the factors controlling soil microbial community structure.

“…(3) According to the standard protocol of Majorbio Bio-Pharm Technology Co., Ltd., (Shanghai, China), on the Illumina MiSeq platform (Illumina, San Diego, CA, USA), the purified amplicons were pooled equimolar and subjected to paired-end sequencing (2 × 250 bp). For the more detailed method, see the reference [30].…”

Section: Soil Microbial Community and Soil Propertiesmentioning

confidence: 99%

Short-Term Effects of Cover Grass on Soil Microbial Communities in an Apple Orchard on the Loess Plateau

Park

2021

Forests

Self Cite

Grass cover may improve soil environmental conditions in apple orchards. However, the mechanisms for how the soil microbial community changes after cover grass treatments are not well understood. In this study, we analyzed soil properties, microbial community diversity and composition in an apple orchard after being covered with native wild grasses for 3 years on the Loess Plateau, China. The ratios of cover grass were 0% (no cover, NC), 20% (low-intensity cover, LIC), 40% (moderate-intensity cover, MIC1), 60% (moderate-intensity cover, MIC2) and 80% (high-intensity cover, HIC). Meanwhile, the relationships between soil nutrients, cover grass properties, and microbial communities was analyzed by redundancy analysis and Pearson correlations. The results showed that cover grass altered the bacterial community composition, and significant changes at the phylum level were mainly caused by Proteobacteria, Bacteroidetes and Chloroflexi. Compared with NC, the abundance of Proteobacteria was lower in LIC, and the abundance of Bacteroidetes was lower in LIC, MIC1 and MIC2, while that of Chloroflexi was higher in LIC. LIC and MIC1 were the only cover grass intensities that altered the soil fungal community composition; there were no significant differences at the phylum level. The changes in the soil microbial community at the given phyla may be related to the change in soil available nitrogen content caused by cover grass. Here, we demonstrate that cover grass changed the soil microbial community, and the changes may be attributed to the given phyla in the bacterial community; soil copiotrophic groups (e.g., Proteobacteria and Bacteroidetes) were found to be at lower abundance in the low-intensity cover grass.