Soil microbial community responses to long-term nitrogen addition at different soil depths in a typical steppe

Niu, Guoxiang; Hasi, Muqier; Wang, Ruzhen; Wang, Yinliu; Geng, Qian; Hu, Shuya; Xu, Xiaohui; Yang, Junjie; Wang, Changhui; Han, Xingguo; Huang, Jianhui

doi:10.1016/j.apsoil.2021.104054

Cited by 38 publications

(23 citation statements)

References 56 publications

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“…In this work, P.communis rhizosphere bacterial community α-diversity increased with increasing N and P addition ratios, while non-rhizosphere bacterial community α-diversity decreased. SEM analysis showed that N and P addition ratio indirectly decreased α-diversity by lowering pH in non-rhizosphere soil, which is consistent with previous studies ( Chen et al., 2021 ; Niu et al., 2021 ). Although the N and P addition ratio increased the non-rhizosphere soil TC content, it did not have a significant increase on bacterial community α-diversity.…”

Section: Discussionsupporting

confidence: 92%

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio

Zhang

Sun

et al. 2023

Front. Plant Sci.

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IntroductionHuman activities have increased the nitrogen (N) and phosphorus (P) supply ratio of the natural ecosystem, which affects the growth of plants and the circulation of soil nutrients. However, the effect of the N and P supply ratio and the effect of plant on the soil microbial community are still unclear.MethodsIn this study, 16s rRNA sequencing was used to characterize the response of bacterial communities in Phragmites communis (P.communis) rhizosphere and non-rhizosphere soil to N and P addition ratio.ResultsThe results showed that the a-diversity of the P.communis rhizosphere soil bacterial community increased with increasing N and P addition ratio, which was caused by the increased salt and microbially available C content by the N and P ratio. N and P addition ratio decreased the pH of non-rhizosphere soil, which consequently decreased the a-diversity of the bacterial community. With increasing N and P addition ratio, the relative abundance of Proteobacteria and Bacteroidetes increased, while that of Actinobacteria and Acidobacteria decreased, which reflected the trophic strategy of the bacterial community. The bacterial community composition of the non-rhizosphere soil was significantly affected by salt, pH and total carbon (TC) content. Salt limited the relative abundance of Actinobacteria, and increased the relative abundance of Bacteroidetes. The symbiotic network of the rhizosphere soil bacterial community had lower robustness. This is attributed to the greater selective effect of plants on the bacterial community influenced by nutrient addition.DiscussionPlants played a regulatory role in the process of N and P addition affecting the bacterial community, and nutrient uptake by the root system reduced the negative impact of N and P addition on the bacterial community. The variations in the rhizosphere soil bacterial community were mainly caused by the response of the plant to the N and P addition ratio.

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Section: Discussionsupporting

confidence: 92%

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio

Zhang

Sun

et al. 2023

Front. Plant Sci.

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“…In this study, we also found that high‐N addition significantly increased TN content in the 10–20 cm soil in all sampling years, indicating more N leached down especially under high N addition rate. Our previous study also reported that the contents of NO 3 − ‐N, NH 4 + ‐N and DON in N addition plots were significantly higher as compared with those in control plots even down to the 70–100 cm depth of soil profiles (Niu, Hasi, et al., 2021).…”

Section: Discussionmentioning

confidence: 61%

“…Moreover, with combination of dynamics of soil NO 3 − ‐N, the amounts of base cations leaching out from upper to deeper soil layers would also increase with increasing rates and duration of N addition (Cameron et al., 2013; Doetterl et al., 2018; Niu, Hasi, et al., 2021). Our previous study in the same region also found that 13‐year N addition only decreased the contents of base cations in surface soils while had insignificant effects on those in subsoils (Niu, Hasi, et al., 2021). The significant decrease in soil total and exchangeable contents of base cations under long‐term N addition demonstrated that the decrease in supply potential of soil base cations to plants, which may cause nutrient imbalance and impoverishment under chronic N and incremental N enrichment (Fang et al., 2017; Lucas et al., 2011; R. Wang et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The atmospheric N deposition at the rate of 2 g N m −2 yr −1 was close to the background atmospheric N deposition and the 10 g N m −2 yr −1 was considered as the critical N saturation threshold of ecosystem functioning in this region (Bai et al., 2010; G. R. Yu et al., 2019). The level of 50 g N m −2 yr −1 was also applied to simulate extremely high N fertilization scenario in this agro‐pastoral region (Niu, Hasi, et al., 2021). We selected three durations of time based on a previous study with similar vegetation by Tian et al.…”

Section: Methodsmentioning

confidence: 99%

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Intensity and Duration of Nitrogen Addition Jointly Alter Soil Nutrient Availability in a Temperate Grassland

Niu

Wang

et al. 2022

JGR Biogeosciences

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Increasing N input can alter soil nutrient availability and influence plant growth. Previous studies focused on N addition effects on N and P availability, while less on other mineral nutrients. Besides, how N addition duration affects nutrient availability has remained unclear. Based on a simulative N deposition experiment in a typical steppe with four N addition levels (0, 2, 10, and 50 g m−2 yr−1) under three N addition duration (2, 5, and 10 years), we determined contents of 10 mineral nutrients in surface soils. In the 0–10 cm soil, short‐term N addition (2‐year) significantly increased exchangeable Ca (+7.2%) and decreased exchangeable Mg (−22.5%) as compared with the control, while decreased available Fe, Cu, and Zn, but increased Mn remarkably (+80.4%). Medium‐term N addition (5‐year) significantly raised soil total N and available Fe, Mn and Cu, while decreased total P and exchangeable Ca, Na and Mg. The response patterns of these nutrients were largely similar in the 10–20 cm soil, but were weaker and significant only at high N inputs (50 g m−2 yr−1). Long‐term N addition (10‐year) significantly decreased contents of total base cations (K, Ca, Na, Mg) and micronutrients (Fe, Mn, Cu, Zn) by an average of 32.1% and 20.4%, respectively, across the two soil depths. Influences of pH and plant growth on micronutrients showed remarkable differences among different duration of N addition. These findings indicate that intensity and duration of N addition jointly alter soil nutrient availability and this should be considered in soil nutrient‐cycling modeling.

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“…For example, the addition of biochar and wheat straw to the soil bacterial community may have an in uence on the structure and diversity of the community and increase the diversity and richness of the bacterial community (Li et al 2020;Cmh et al 2020). The addition of exogenous organic materials can promote the growth of some bacteria and also inhibit some other bacteria's growthm, which results in a shift in the bacterial community structure of the soil (Niu et al 2021). From the diversity index table (Table 2), the CN20 treatment improved the variety and richness of the soil bacterial populations to the greatest extent.…”

Section: Effects Of C/n Ratio Organic Materials On the Soil Physicoch...mentioning

confidence: 99%

Organic materials with high C/N ratio: more beneficial to soil improvement and soil health

Ren

et al. 2022

Biotechnol Lett

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In order to gure out the effect of organic fertilizers with different carbon-nitrogen (C/N) ratios on the soil improvement and the healthy cultivation, the pot experiment method was used to study effects on the physical and chemical properties and the bacterial community structure of sandy loam soil using ve treatments of chemical fertilizer application with the C/N ratios of 15 (CN15), 20 (CN20), 25 (CN25), 30 (CN30) and the control (CK) respectively. Results show that the organic materials with different C/N ratios signi cantly improve the soil porosity and water content, which all show a linear change rule with the C/N ratio. It can also signi cantly increase the soil total carbon, total nitrogen, soil C/N ratio, soil microbial biomass carbon, microbial biomass nitrogen and microbial biomass C/N ratio. Among them, CN30 signi cantly increases the soil total carbon and C/N ratio, which are 5.34%-24.13% and 8.87%-30.15% respectively compared with other treatments. It can be also found that the dominant ora (at the phylum level) of each treatment are Actinobacteria, Proteobacteria and Chlorobi. The CN30 treatment presents the most obvious improvement in the diversity and richness of the soil bacterial community and is more conducive to the growth and reproduction of Proteobacteria and Firmicutes. The correlation analysis shows that C total /N total and C mic /N mic are the most important environmental factors affecting the soil physical and chemical properties and their correlation with the bacterial communities. The higher C/N ratio of organic materials results in a more signi cant improvement of the soil physical and chemical properties. This study provides a new theoretical basis for the soil health cultivation technology.

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Soil microbial community responses to long-term nitrogen addition at different soil depths in a typical steppe

Cited by 38 publications

References 56 publications

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio

Intensity and Duration of Nitrogen Addition Jointly Alter Soil Nutrient Availability in a Temperate Grassland

Organic materials with high C/N ratio: more beneficial to soil improvement and soil health

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