Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India

Yadav, Arun K.; Lalramliana,

doi:10.1007/s12639-011-0076-x

Cited by 16 publications

(9 citation statements)

References 19 publications

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“…There are certain species of Steinernema that can infect the host in soil with moisture level as low as 1% (Gungor et al, 2006) and 2% moisture level (Kung, 1990). In the present study, the highest numbers of established IJ were observed at 10% and 15% moisture level which is in close agreement with reports for H. indica strain T2 (Maketon et al, 2011), H. indica isolate from Meghalaya, India (Yadav, 2012) and H. bacteriophora (Susurluk et al, 2001). Further increase in water content resulted in decline in number of invading IJ indicating that higher moisture level can have detrimental effects on the control potential of EPN (Koppenhöfer et al, 1995).…”

Section: Discussionsupporting

confidence: 93%

“…Ecological characterisation of H. indica isolated from different geographical regions have been reported previously (Ebssa et al, 2004;Iraki et al, 2000;Karunakar et al, 1999;Ma et al, 2013;Nguyen et al, 2006;Noosidum et al, 2010;Shamseldean et al, 1996;Shapiro-Ilan et al, 2005;Shapiro-Ilan et al, 2014;Shapiro-Ilan et al, 2009;Walia et al, 2008;Xu et al, 2010;Yadav, 2012). In the present study, we provide the ecology of the EPN H. indica isolated from the island of Viti Levu, Fiji.…”

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confidence: 72%

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Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Kour

Khurma

Brodie

2021

Journal of Nematology

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Entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are obligate parasites of soil inhibiting insects. EPNs are being widely researched as promising biocontrol agents for a wide range of agricultural pests. It is known that strains of EPNs isolated from different geographical regions differ in their attributes, such as host-finding ability, host range, infectivity, reproduction, and environmental stress tolerance. A precise knowledge of these factors is therefore an essential pre-requisite for devising successful strategies to use these nematodes in biological control programmes. Thus, ecological characterisation of the EPN Heterorhabditis indica (Rhabditida: Heterorhabditidae) newly isolated and representing the only species of EPN reported from the island of Viti Levu, Fiji was carried out using Galleria mellonella larvae (L) (Pyralidae: Galleriinae) as hosts to allow comparisons between bioassays conducted in different laboratories around the world. Temperature data showed that native isolates of H. indica are warm-adapted nematodes with thermal range for infectivity between 15˚C and 35˚C and can reproduce between 20˚C and 30˚C. They are highly virulent with LC50 values against G. mellonella ranging from 2.8 IJ to 3.8 IJ/larva. However, they showed poor desiccation tolerance and fail to infect hosts in soil with moisture levels below 8%. They showed a moderate level of hypoxic tolerance and can be stored at 15˚C for 4 months. Results also showed great variability within the selected native isolates of H. indica. Beneficial traits for selected isolates were added up to identify a superior candidate. The current study also suggested that the thermal niche breadth for infection can differ among conspecific strains of an EPN species. The results of this experimental study on ecological aspects of these native isolates of H. indica should form a basis for their potential use in biological control of insect pests in Fiji.

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Section: Discussionsupporting

confidence: 93%

supporting

confidence: 72%

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Kour

Khurma

Brodie

2021

Journal of Nematology

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“…The differences in the number of larvae emerging from one insect observed between the bioassays are confirmed by the other studies which demonstrated that the number of infective juveniles may fluctuate from few to few dozen thousand (Mason & Hominick 1995;Strong et al 1996). The differences in biology of nematodes isolated from the natural environment was also confirmed by the scientists conducting research on isolates of S. feltiae originating from Spain (Campos-Herrera et al 2006;Yadav & Lalramliana 2012).…”

Section: Resultssupporting

confidence: 52%

Effectiveness of selected strains of entomopathogenic nematodes in peat substrate in container breeding

Matuska-Łyżwa

2013

Ecological Questions

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Abstract. Pest control in urban green areas is particularly difficult due to the limitation of pesticide use. Using efficient methods for biological control of herbivores there may be a good solution. Planting plants growing in the forest container breeding have a better growth and better root system comparing to the seedlings produced in traditional forest nurseries. Such plants are not subject to significant shock when replanted, however, they are still exposed to pests living near plantings. Our research assessed using entomopathogenic nematodes to be introduced into the soil used for plantings in order early protect seedlings against their pests. Nematodes isolated from the natural environment as well as, for control samples, from biopreparations were used in the study. The research was conducted on the soil substrate in containers with seedlings. During the experiment were taken the following parameters: insect mortality, nematode invasion extensity, migration time of invasive larvae, number of migrating larvae, and their survival.The results showed that entomopathogenic nematodes isolated from various provinces in Poland were effective, but characterized by a diverse insecticidal activity in a peat substrate.

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“…It is widely recognized now that the selection of a suitable EPN species as a biocontrol agent in a given geographical area requires adequate knowledge about the biology and ecology of the nematode. Information relating to the above three EPN isolates, such as the effects of soil moisture on the establishment and the effects of relative humidity on the emergence and reproduction were studied by Lalramliana and Yadav (2009) and Yadav and Lalramliana (2012c). Among other abiotic factors, the temperature is also considered as an important factor in determining the suitability of EPN as an appropriate biocontrol agent.…”

Section: Introductionmentioning

confidence: 99%

Effects of storage temperature on survival and infectivity of three indigenous entomopathogenic nematodes strains (Steinernematidae and Heterorhabditidae) from Meghalaya, India

Lalramliana

Yadav

2015

J Parasit Dis

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Three locally isolated strains of entomopathogenic nematodes (EPNs), viz. Heterorhabditis indica, Steinernema thermophilum and Steinernema glaseri, from Meghalaya, India were characterized in terms of storage temperature and survival and infectivity of their infective juveniles (IJs). The survival and infectivity of nematode IJs was studied at, 5 ± 2 and 25 ± 2°C, for a period of 120 days, using deionized water as storage medium. The viability of nematode IJs was checked by mobility criterion at different storage periods, while the infectivity of nematode IJs was ascertained on the basis of establishment of IJs, using Galleria mellonella larva mortality tests in petridishes. The results of this study revealed that storage temperature markedly affects the survival as well as the establishment of nematode IJs of the three EPN species. At 5°C, comparatively higher rate of IJ's survival (i.e. 74-86 %) was observed for 15 days of storage, but the same reduced drastically to 28-32 % after 30 days of storage for H. indica and S. thermophilum. On the other hand, at 25°C, the survival of nematode IJs was observed till 120 days for all the three studied EPNs. In case of S. thermophilum and S. glaseri, higher rate of IJs survival ([75 %) was observed respectively at 15 and 30 days of observation. The study also showed that the establishment of IJs of the three EPN species declines with increase in storage periods, at both the test temperatures. In general, the nematodes stored at 25°C showed comparatively better establishment than those stored at 5°C. Among the three EPN studied, the establishment of S. glaseri was comparatively better than the rest of the species at both the temperatures and for different storage durations. In conclusion, our study adds further valuable information about the effect of storage temperature on survival and infectivity of three indigenous EPN species of Meghalaya, India which appears to be promising biocontrol agents of local insect pests.

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Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India

Abstract: Entomopathogenic nematodes (EPNs)

Cited by 16 publications

References 19 publications

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Effectiveness of selected strains of entomopathogenic nematodes in peat substrate in container breeding

Effects of storage temperature on survival and infectivity of three indigenous entomopathogenic nematodes strains (Steinernematidae and Heterorhabditidae) from Meghalaya, India

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