Aim: To study the impact of altitudinal variation on plant community composition, structure, dispersion and regeneration status of Quercus semecarpifolia forest in Kumaun Himalaya. Methodology: Along the altitudinal gradient, the forest stands between 2400 and 2610 m asl were selected at low, mid and high altitude. The phyto-sociological analysis was carried by laying ten quadrats of 10m × 10m at each site. Soil samples were collected with the help of soil corer from two depths. Various ecological indices and population structure were investigated for each forest stand and regeneration status of forest was predicted by the population size of seedlings, saplings and trees. Tree biomass was estimated using allomatric equations and carbon stock was determined by multiplying biomass of species to factor 0.475. Results: With increasing altitude the number of tree species decreased and the shrub species richness increased, while herb species showed a unimodel pattern. Q. semecarpifolia was the dominant tree species at all the three sites with the IVI values of 220.14, 255.22 and 286.23 at LA, MA and HA, respectively. A complete absence of Q. semecarpifolia seedlings indicated no regeneration in low and high altitude stands while low proportion of seedlings in mid altitude forest stand indicated poor regeneration. Soil was acidic (pH 5.66-5.86), with higher silt content and showed decreasing pattern in physico-chemical properties with increasing altitude. The biomass of tree layer ranged from 871.49 to 1050.17 t ha-1. The tree layer carbon stock was maximum in high altitude forest (498.84 t ha-1) which was largely contributed by bole, stump roots and branches. Interpretation: Variation in species richness, distribution pattern and regeneration potential is related to site characteristics governed by altitude and require various efforts to conserve and protect these forests to check ecosystem imbalance.