Most tropical soils have high acidity and low natural fertility. The appropriate application of lime and cattle manure corrects acidity, improves physical and biological properties, increases soil fertility, and reduces the use of chemical and/or synthetic fertilizers by crops, such as soybean, the main agricultural export product of Brazil. This study aimed to assess the effects of the combination of the application of dolomite limestone (0, 5, and 10 Mg ha −1 ) and cattle manure (0, 40, and 80 Mg ha −1 ) on grain yield and the chemical properties of an Oxisol (Red Latosol) cultivated with soybean for two consecutive years. The maximum grain yield was obtained with the application of 10 Mg ha −1 of lime and 80 Mg ha −1 of cattle manure. Liming significantly increased pH index, the concentrations of calcium (Ca 2+ ) and exchangeable magnesium (Mg 2+ ), and cation exchange capacity (CEC) of soil and reduced potential acidity (H + + Al 3+ ), while the application of cattle manure increased pH level; the concentrations of potassium (K + ), Ca 2+ , and exchangeable Mg 2+ ; and CEC of the soil. During the 2 years of assessment, the greatest grain yields were obtained with saturation of K + , Ca 2+ , and Mg 2+ in CEC at the 4.4, 40.4, and 17.5 levels, respectively. The results indicated that the ratios of soil exchangeable Ca/Mg, Ca/K, K/Mg, and K/(Ca+Mg) can be modified to increase the yield of soybean grains.