The Soil Management Assessment Framework (SMAF) was developed to evaluate impacts of land use and management practices on soil quality (SQ), but its suitability for Brazilian tropical soils was unknown. We hypothesized that SMAF would be sensitive enough to detect SQ changes associated with sugarcane (Saccharum officinarum L.) expansion for ethanol production. Field studies were performed at three sites across the south-central region of Brazil, aiming to quantify the impacts of a land use change sequence (i.e., native vegetation-pasture-sugarcane) on SQ. Eight soil indicators were individually scored using SMAF curves developed primarily for North American soils and integrated into an overall Soil Quality Index (SQI) and its chemical, physical, and biological sectors. The SMAF scores were correlated with two other approaches used to assess SQ changes, soil organic C (SoC) stocks and Visual Evaluation of Soil Structure (VESS) scores. our findings showed that the SMAF was an efficient tool for assessing land use change effects on the SQ of Brazilian tropical soils. The SMAF scoring curves developed using robust algorithms allowed proper assignment of scores for the soil chemical, physical, and biological indicators assessed. The SQI scores were significantly correlated with SoC stocks and VESS scores. Long-term transition from native vegetation to extensive pasture promoted significant decreases in soil chemical, physical, and biological indicators. overall SQI suggested that soils under native vegetation were functioning at 87% of their potential capacity, while pasture soils were functioning at 70%. Conversions of pasture to sugarcane induced slight improvements in SQ, primarily because of improved soil fertility. Sugarcane soils are functioning at 74% of their potential capacity. Based on this study, management strategies were developed to improve SQ and the sustainability of sugarcane production in Brazil.Abbreviations: AGS, macroaggregate stability; BD, bulk density; BG, b-glucosidase activity; MBC, microbial biomass carbon; SMAF, Soil Management Assessment Framework; SQ, soil quality; SQI, soil quality index; SOC, soil organic carbon; VESS, Visual Evaluation of Soil Structure. S oil quality or health is a key factor required to achieve sustainable agricultural systems that will meet our increasing demands for food, feed, fiber, and fuels. Therefore, in recent decades SQ has been discussed worldwide and become a major agenda item for the scientific community (Karlen et al., , 2014a. Soil quality was defined as the capacity of a specific kind of soil to function, within natural or managed ecosystem boundaries, to sustain plant and animal productivity, maintain or enhance water and air quality, and support human health and habitation (Karlen et al., 1997 Core Ideas:• The SMAF efficiently detected soil quality changes under Brazilian tropical conditions.• Soil Quality Index was 0.87 (native vegetation), 0.70 (pasture), and 0.74 (sugarcane).• Sugarcane expansion improves soil quality, mainly due to increasing so...