Understanding the spatio-temporal variability of soil water content (SWC) in the Critical Zone (CZ) is essential to balance plant-water benefits and to enhance the ability to monitor and predict drought. In current study, the spatial variability and temporal stability of multi-layer SWC to a depth of 500 cm (35 soil layers) at 27 sampling sites were evaluated at a hillslope scale. A total of 64 SWC measurements were obtained from 28 March, 2017 to 23 December, 2018. We hypothesized the existence of a "special point" that is temporally stable both in horizontal direction (which means different layers) and in vertical direction (which means different profiles). We found a clear seasonal dynamic pattern of mean SWC for all sites in the 0-500 cm profile, with SWC varying from 4.0 to 24.8%. In the horizontal layer, both the 130-cm and 140-cm layers contained a maximum number of temporal stability sites (N = 4) which all located at H 11 , H 13 , H 14 and H 26. In the vertical profile, H 19 was the most stable site, followed by H 10 and H 26 , with 15, 10 and 6 stable layers, respectively. The decreasing order of SWC temporal stability was 200-500 cm, 0-500 cm and 0-200 cm, which means the temporal stability of SWC in 0-500 cm profile was more dependent on the 0-200 cm sub-profile. At a three-dimension scale, the most temporal stability layer and site was 180-cm of H 1 and H 21 of 220-cm, which confirmed our hypothesis. This study provides an accurate description of SWC spatio-temporal variation at the hillslope scale, which is helpful for addressing eco-hydrological models and water management issues in the Earth's CZs.