The agrosoddy-podzolic soil (Eutric Albic Glossic Retisol (Abruptic, Loamic, Aric, Cutanic)) is typical for Moscow Oblast and is used for agricultural purposes, resulting in use of various agrochemicals and pesticides. The presence of macropores and cracks in such soils leads to preferential water and substance transfer and nonequilibrium conditions. Therefore, it is important to study the numerical characteristics of the pore space of soils to adjust mathematical models of substance transfer. Undisturbed soil monoliths 10 cm in diameter taken from Ap (from 0 to 30 cm) and E, BE horizons (from 30 to 50 cm) were investigated under the field moisture conditions and after saturation using the tomographic core analyzer RKT-180 with the resolution of 200 μm/pixel. Using the X-ray computer tomography, it has been established that the plough layer of the agrosoddy-podzolic soil contains over 7% of macropores larger than 1 mm, while the subsurface layer has a porosity of about 3%. After saturation, some of the inter-aggregate pores overlap, which leads to a decrease in the total porosity to 4% in the upper and 2% in lower horizons, as well as increase in the average pore diameter. The number of macropores determined by tomographic analysis is one third higher than the values calculated using pedotransfer functions for this soil. The data obtained in this paper are recommended for use in national scenarios of migration of substances (pesticides, agrochemicals, salts) in soils.