Sol-gel-derived 58S bioactive glass containing holmium aiming brachytherapy applications: A dissolution, bioactivity, and cytotoxicity study

Delpino, Giulia Piagentini; Borges, Roger; Zambanini, Telma; Joca, Jhonny Frank Sousa; Gaubeur, Ivanise; Souza, Ana Carolina Santos de; Marchi, Juliana

doi:10.1016/j.msec.2020.111595

Cited by 30 publications

(19 citation statements)

References 40 publications

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“…Because the holmium content in the glasses is responsible for modulating the nuclear properties for brachytherapy applications, we developed a series of glasses with different holmium content, which could be used for bone cancer treatment following the dose rate designed for each patient. Our research group has already characterized these glasses regarding their dissolution, bioactivity, and cytotoxicity to MC3T3 cell lineage [ 46 ]. In this previous work, we also showed that holmium was incorporated in the glass network without any devitrification.…”

Section: Discussionmentioning

confidence: 99%

“…These compositions have been studied by our research group, derived from the 58S bioactive glass [ 59 ]. They show high bioactivity and biocompatibility towards pre-osteoblastic cell (MC3T3) [ 46 ]. For the synthesis, tetraethylorthosilicate (TEOS—Sigma-Aldrich, St. Louis, MO, USA 99.99%, CAS # 78-10-8) and triethyl phosphate (TEP—Sigma-Aldrich, St. Louis, MO, USA, >99.8%, CAS # 78-40-0) were used as precursors of SiO 2 and P 2 O 5 , respectively, and calcium nitrate tetrahydrate (Ca(NO 3 ) 3 .4H 2 O—Sigma-Aldrich, St. Louis, MO, USA, >99.0%, CAS # 13477-34-4) and holmium nitrate pentahydrate (Ho(NO 3 ) 3 .5H 2 O—Sigma-Aldrich, St. Louis, MO, USA, 99.9%, CAS #14483-18-2) were used as precursors of CaO, and Ho 2 O 3 , respectively.…”

Section: Methodsmentioning

confidence: 99%

“…For the synthesis, tetraethylorthosilicate (TEOS—Sigma-Aldrich, St. Louis, MO, USA 99.99%, CAS # 78-10-8) and triethyl phosphate (TEP—Sigma-Aldrich, St. Louis, MO, USA, >99.8%, CAS # 78-40-0) were used as precursors of SiO 2 and P 2 O 5 , respectively, and calcium nitrate tetrahydrate (Ca(NO 3 ) 3 .4H 2 O—Sigma-Aldrich, St. Louis, MO, USA, >99.0%, CAS # 13477-34-4) and holmium nitrate pentahydrate (Ho(NO 3 ) 3 .5H 2 O—Sigma-Aldrich, St. Louis, MO, USA, 99.9%, CAS #14483-18-2) were used as precursors of CaO, and Ho 2 O 3 , respectively. Glasses were obtained by modifying the sol-gel quick alkali route described in [ 46 , 60 ], which allows the obtainment of glass nanoparticles. Briefly, TEOS and TEP were hydrolyzed in a solution containing deionized water, ethanol, and 2 M HNO 3 (Sigma-Aldrich, St. Louis, MO, USA, 70%, CAS #7697-37-2) in a 13.9:50:2 ratio.…”

Section: Methodsmentioning

confidence: 99%

“…Besides their bioactivity, when these glasses interact with the body fluid, their surface is dissolved, and the dissolution products can promote specific biological responses [3]. For example, their holmium is leached into the body over slower dissolution kinetics, which guarantees the safety of using them as radioactive elements in the glass, once 166 Ho will not be leached into the body at concentrations enough to damage healthy cells [46]. Because these glasses can; (i) regenerate the bone loss caused by cancer through their osteoinduction and bioactivity, (ii) besides treating the bone cancer by brachytherapy, these glasses can be defined as theragenerative biomaterials [47].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we showed that holmium ions in bioactive glasses based on the system SiO 2 -CaO-P 2 O 5 -Ho 2 O 3 are responsible for stronger Si-O-Ho chemical bonds but lower glass connectivity, enabling proper glass dissolution kinetics to favor bioactivity [ 46 ]. However, given that the strong covalent bonds between holmium and adjacent non-bridging oxygens, holmium is leached into the body over slower dissolution kinetics, which guarantees the safety of using them as radioactive elements in the glass, once 166 Ho will not be leached into the body at concentrations enough to damage healthy cells [ 46 ]. Because these glasses can; (i) regenerate the bone loss caused by cancer through their osteoinduction and bioactivity, (ii) besides treating the bone cancer by brachytherapy, these glasses can be defined as theragenerative biomaterials [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

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Holmium-Containing Bioactive Glasses Dispersed in Poloxamer 407 Hydrogel as a Theragenerative Composite for Bone Cancer Treatment

et al. 2021

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Holmium-containing bioactive glasses can be applied in bone cancer treatment because the holmium content can be neutron activated, having suitable properties for brachytherapy applications, while the bioactive glass matrix can regenerate the bone alterations induced by the tumor. To facilitate the application of these glasses in clinical practice, we proposed a composite based on Poloxamer 407 thermoresponsive hydrogel, with suitable properties for applications as injectable systems. Therefore, in this work, we evaluated the influence of holmium-containing glass particles on the properties of Poloxamer 407 hydrogel (20 w/w.%), including self-assembly ability and biological properties. 58S bioactive glasses (58SiO2-33CaO-9P2O5) containing different Ho2O3 amounts (1.25, 2.5, 3.75, and 5 wt.%) were incorporated into the hydrogel. The formulations were characterized by scanning electron microscopy, differential scanning calorimetry, rheological tests, and [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] MTT cell viability against pre-osteoblastic and osteosarcoma cells. The results evidenced that neither the glass particles dispersed in the hydrogel nor the holmium content in the glasses significantly influenced the hydrogel self-assembly ability (Tmic ~13.8 °C and Tgel ~20 °C). Although, the glass particles considerably diminished the hydrogel viscosity in one order of magnitude at body temperature (37 °C). The cytotoxicity results evidenced that the formulations selectively favored pre-osteoblastic cell proliferation and osteosarcoma cell death. In conclusion, the formulation containing glass with the highest fraction of holmium content (5 wt.%) had the best biological results outcomes aiming its application as theragenerative materials for bone cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Holmium-Containing Bioactive Glasses Dispersed in Poloxamer 407 Hydrogel as a Theragenerative Composite for Bone Cancer Treatment

et al. 2021

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Biocompatible Glasses Applied in Cancer Treatment: Magnetic Hyperthermia and Brachytherapy

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Bioactive Glasses and Glass‐Ceramics

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Nanostructured bioactive glasses: A bird's eye view on cancer therapy

Kargozar

Moghanian

Rashvand

et al. 2023

WIREs Nanomed Nanobiotechnol

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Bioactive glasses (BGs) arewell known for their successful applications in tissue engineering and regenerative medicine. Recent experimental studies have shown their potential usability in oncology, either alone or in combination with other biocompatible materials, such as biopolymers. Direct contact with BG particles has been found to cause toxicity and death in specific cancer cells (bone‐derived neoplastic stromal cells) in vitro. Nanostructured BGs (NBGs) can be doped with anticancer elements, such as gallium, to enhance their toxic effects against tumor cells. However, the molecular mechanisms and intracellular targets for anticancer compositions of NBGs require further clarification. NBGs have been successfully evaluated for use in various well‐established cancer treatment strategies, including cancer hyperthermia, phototherapy, and anticancer drug delivery. Existing results indicate that NBGs not only enhance cancer cell death, but can also participate in the regeneration of lost healthy tissues. However, the application of NBGs in oncology is still in its early stages, and numerous unanswered questions must be addressed. For example, the impact of the composition, biodegradation, size, and morphology of NBGs on their anticancer efficacy should be defined for each type of cancer and treatment strategy. Moreover, it should be more clearly assessed whether NBGs can shrink tumors, slow/stop cancer progression, or cure cancer completely. In this regard, the use of computational studies (in silico methods) is highly recommended to design the most effective glass formulations for cancer therapy approaches and to predict, to some extent, the relevant properties, efficacy, and outcomes.This article is categorized under: Implantable Materials and Surgical Technologies > Nanomaterials and Implants Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease

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Sol-gel-derived 58S bioactive glass containing holmium aiming brachytherapy applications: A dissolution, bioactivity, and cytotoxicity study

Cited by 30 publications

References 40 publications

Holmium-Containing Bioactive Glasses Dispersed in Poloxamer 407 Hydrogel as a Theragenerative Composite for Bone Cancer Treatment

Holmium-Containing Bioactive Glasses Dispersed in Poloxamer 407 Hydrogel as a Theragenerative Composite for Bone Cancer Treatment

Biocompatible Glasses Applied in Cancer Treatment: Magnetic Hyperthermia and Brachytherapy

Nanostructured bioactive glasses: A bird's eye view on cancer therapy

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