Solanum�nigrum Linne improves DNCB‑induced atopic dermatitis‑like skin disease in BALB/c mice

Abstract

The present study aimed to investigate the effects of Solanum nigrum linne (Snl) in a model of 1-chloro-2,4-dinitrobenzene (dncB)-induced atopic dermatitis (ad) and in TnF-α/iFn-γ-stimulated HacaT cells. AD is a chronic inflammatory skin disease and is characterized by erythema, edema, increased pruritus and eczema. Steroids are most commonly used for anti-inflammatory therapy; however, their long-term use is limited due to side-effects, such as osteoporosis, brittle skin, muscle weaknesses and diabetes. Therefore, patients with ad require alternative treatment strategies. in previous studies, Snl has been reported to be effective against oxidants and cancer. However, to the best of our knowledge, the effects of SNL on AD have not yet been investigated. The present study examined the effects of Snl ethanol extract on a model of dncB induced ad and on TnF-α/iFn-γ-stimulated HacaT cells. The skin tissue was sectioned to measure the thicknesses of the epidermis and dermis, as well as the numbers of eosinophils, mast cells and cd8 infiltration by H&e, toluidine blue, Masson's trichrome and iHc staining. eliSa was performed using serum to measure ige levels. The present study also examined the expression of various inflammatory cytokines, MaPK and nF-κB in TnF-α/iFn-γ-stimulated HacaT cells. SNL significantly reduced the levels of cytokines released from HacaT cells stimulated with TnF-α/iFn-γ. Snl also significantly reduced the levels of p-p38 at 30 min and significantly reduced the activation of NF-κB in a time course experiment. In addition, SNL significantly reduced the level of serum IgE and dermal thickness and the infiltration of mast cells and cd8 in the BalB/c mouse model of dncB-induced ad. The results of the current study suggest that Snl exerts a suppressive effect on pro-inflammatory cytokines in vitro and in vivo through the regulation of the immune system.

Immunomodulatory and Anti-inflammatory Effects of Asiatic Acid in a DNCB-Induced Atopic Dermatitis Animal Model

“…However, AA treatment dose-dependently reduced collagen fiber deposition and epidermal and dermal thickness ( Figure 5 ). Our results are consistent with those of a previous study showing that Solanum nigrum L. treatment downregulates mast cell infiltration and epidermal and dermal thickness in a DNCB-induced AD animal model [ 28 ]. Thus, AA treatment regulated mast cells infiltration and skin lesion formation in AD, which might explain the recovery from AD skin lesions.…”

The Effect and Mechanism of Burnet Gels on Steroid-Dependent Dermatitis in Guinea Pig Model

“…The results of this study showed that the HDD guinea pig model showed hyperplasia of epidermis, parakeratosis in the stratum corneum, and decreased expression of epidermis-related proteins, which may be related to the formation of inflammatory cascades. IL-4 and IFN-γ can induce the apoptosis of HDD keratinocytes to increase and damage the skin barrier, reduce the expression of FLG, LOR, and Caspase-14 in the epidermis, continuously aggravate the degree of skin lesions, and prolong the duration of the skin lesions, causing the epidermis to increase thickness and parakeratosis [16][17][18].…”

β-Nicotinamide Mononucleotide (NMN) Administrated by Intraperitoneal Injection Mediates Protection Against UVB-Induced Skin Damage in Mice

“…The liver was stained with H&E, and the skin was stained with Masson's trichrome, H&E, and toluidine blue (TB). 31 Generally speaking, the liver and skin tissues are first fixed with 10% formaldehyde, then dehydrated with alcohol, transparent with xylene, and embedded in paraffin. Then, the embedded wax block was cut into 5-8 micron slices with a microtome, and dried at a constant temperature at 45 °C.…”