Solar Powered Electro-Bio-Stabilization of Soil with Ammonium Pollution Prevention System

Keykha, Hamed A.; Asadi, Afshin

doi:10.1520/acem20170001

Cited by 15 publications

(11 citation statements)

References 28 publications

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“…The limitations associated with its prospective eld application are ammonium production as a byproduct, non-uniformity of precipitation, and transport of healthy ureolytic bacteria in large quantities to the site 18,22 . While the potential strategies to negate a higher concentration of ammonium ions and non-uniformity are being investigated comprehensively [23][24][25][26][27][28] , the transport of calcifying bacterial culture to the desired site is largely unaddressed. The presence of native ureolytic bacteria and their enrichment on-site can tackle the challenge of bacteria transport 29,30 .…”

Section: Introductionmentioning

confidence: 99%

Biocementation Mediated By Stimulated Ureolytic Microbes From Brahmaputra Riverbank For Mitigation of Soil Erosion

Dubey

Ravi

Mukherjee

et al. 2021

Preprint

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Riverbank erosion is a global problem with significant socio-economic impacts. Microbially induced carbonate precipitation (MICP) has recently emerged as a promising technology for improving the mechanical properties of soils. The presented study investigates the potential of native calcifying bacterial communities of the Brahmaputra riverbank for the first time via biostimulation and explores its effect on the mitigation of soil erosion. The ureolytic and calcium carbonate cementation ability of the enriched cultures were investigated with reference to the standard calcifying culture of Sporosarcina pasteurii (ATCC 11859). 16S rRNA analysis revealed Firmicutes to be the most predominant calcifying class with Sporosarcina pasteurii and Pseudogracilibacillus auburnensis as the prevalent strains. The morphological and mineralogical characterization of carbonate crystals confirmed the calcite precipitation potential of these communities. The erosion resistance of soil treated with native calcifying communities was examined via needle penetration and lab-scale flume erosion test. We found a substantial reduction in soil erosion in the biocemented sample with calcite content of 7.3% and needle penetration index of 16 N/mm. We report cementation potential of biostimulated ureolytic cultures for a cost-competitive and environmentally-conscious alternative to current erosion mitigation practices.

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Section: Introductionmentioning

confidence: 99%

Biocementation Mediated By Stimulated Ureolytic Microbes From Brahmaputra Riverbank For Mitigation of Soil Erosion

Dubey

Ravi

Mukherjee

et al. 2021

Preprint

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“…However, these alternatives have certain disadvantages and therefore may not be suitable for bio‐cementation applications; (ii) injecting tap water to flush the produced ammonium, and then extracting the discharged solution by wells is also an effective way (Van Paassen et al . 2009), (iii) a column electrokinetic cell composed of graphite anode/cathode electrode was used to retain

{NH}_{4}^{+}

in the cathode chamber, preventing leakage of

{NH}_{4}^{+}

into soil (Keykha and Asadi 2017), (iv) When

{normalK}_{2} {HPO}_{4} \cdot 3 {normalH}_{2} O

is added into bacterial solutions and

{CaCl}_{2}

was replaced by

{MgCl}_{2}

{NH}_{4} {MgPO}_{4} \cdot 6 {normalH}_{2} O

will precipitate, which can fix 88·52% of

{NH}_{4}^{+}

(Yu et al . 2019), etc.…”

Section: Introductionmentioning

confidence: 99%

Microbially induced carbonate precipitation via methanogenesis pathway by a microbial consortium enriched from activated anaerobic sludge

Yang

2020

J. Appl. Microbiol.

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Aims Various applications of microbially induced carbonate precipitation (MICP) has been proposed. However, most studies use cultured pure strains to obtain MICP, ignoring advantages of microbial consortia. The aims of this study were to: (i) test the feasibility of a microbial consortium to produce MICP; (ii) identify functional micro‐organisms and their relationship; (iii) explain the MICP mechanism; (iv) propose a way of applying the MICP technique to soil media. Methods and Results Anaerobic sludge was used as the source of the microbial consortium. A laboratory anaerobic sequencing batch reactor and beaker were used to perform precipitation experiment. The microbial consortium produced MICP with an efficiency of 96·6%. XRD and SEM analysis showed that the precipitation composed of different‐size calcite crystals. According to high‐throughput 16S rRNA gene sequencing, the functional micro‐organisms included acetogenic bacteria, acetate‐oxidizing bacteria and archaea Methanosaeta and Methanobacterium beijingense. The methanogenesis acetate degradation provides dissolved inorganic carbon and increases pH for MICP. A series of reactions catalysed by many enzymes and cofactors of methanogens and acetate‐oxidizers are involved in the acetate degradation. Conclusion This work demonstrates the feasibility of using the microbial consortium to achieve MICP from an experimental and theoretical perspective. Significance and Impact of the Study A method of applying the microbial‐consortium MICP to soil media is proposed. It has the advantages of low cost, low environmental impact, treatment uniformity and less limitations from natural soils. This method could be used to improve mechanical properties, plug pores and fix harmful elements of soil media, etc.

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“…Other findings relate to the injection of calcium ions at the anode and carbonate ions originating from microbially hydrolysed urea at the cathode which results into a good rate of calcification throughout soft clay (kaolinite) by means of EKs transport (electromigration and electroosmosis) of the reactive species (Keykha et al 2014). In addition, EKs also seem to be a promising way of removing ammonium ions which can potentially become a source of environmental contamination (Keykha et al 2017). The present study focuses on the potentially detrimental and beneficial effects of applied EKs to suggest an efficient EA-MICP approach.…”

Section: Introductionmentioning

confidence: 99%

Benefits and drawbacks of applied direct currents for soil improvement via carbonate mineralization

2020

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The study presented herein adopts a new vision of the processes involved in carbonate mineralization induced by MICP from an electrochemical and crystal growth perspective. More precisely a specific line of focus refers to the species involved in the bio-chemical reactions and especially their net particle charge. By altering electro-chemical conditions via the application of direct electric currents, we observe distinctive trends related to: (i) overall reaction efficiency; (ii) carbonate mineralization/dissolution and (iii) spatial distribution of precipitates. The study introduces the concept of EA-MICP which stands for Electrically Assisted MICP as a means of improving the efficiency of soil bio-consolidation and overcoming various challenges which were previously reported in conventional MICP-based works. Results reveal both the detrimental and highly beneficial role that electric currents can hold in the complex, reactive and transport processes involved. An interesting finding is the “doped” morphology of calcite crystals, precipitated under electric fields, validated by microstructural observations.

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Solar Powered Electro-Bio-Stabilization of Soil with Ammonium Pollution Prevention System

Cited by 15 publications

References 28 publications

Biocementation Mediated By Stimulated Ureolytic Microbes From Brahmaputra Riverbank For Mitigation of Soil Erosion

Biocementation Mediated By Stimulated Ureolytic Microbes From Brahmaputra Riverbank For Mitigation of Soil Erosion

Microbially induced carbonate precipitation via methanogenesis pathway by a microbial consortium enriched from activated anaerobic sludge

Benefits and drawbacks of applied direct currents for soil improvement via carbonate mineralization

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