Solar-to-bioH2 production enhanced by homologous overexpression of hydrogenase in green alga Chlorella sp. DT

Chien, Lee-Feng; Kuo, Ting-Ting; Liu, Bang-Hong; Lin, Hsin-Di; Feng, Ting-Yung; Huang, Chieh-Chen

doi:10.1016/j.ijhydene.2012.09.068

Cited by 37 publications

(18 citation statements)

References 41 publications

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“…Engineering has also focused on indirect targets, including electron competitors such as RuBisCo (Pinto et al ., ), cyclic electron flow (Kruse et al ., ; Tolleter et al ., ), starch degradation (Chochois et al ., ) and respiration (Ruehle et al ., ), with mutants in all of these pathways reportedly yielding increased H 2 production levels. Finally, additional components have been added to the electron transfer pathway, including a plastid‐expressed NAD(P)H dehydrogenase (Baltz et al ., ), a hexose transporter (Doebbe et al ., ) and exogenous hydrogenases (Chien et al ., ).…”

Section: Biological Challenges and Bottlenecks For Microalgal H2 Prodmentioning

confidence: 97%

Challenges and opportunities for hydrogen production from microalgae

Oey

Sawyer

Ross

et al. 2016

Plant Biotechnology Journal

145

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SummaryThe global population is predicted to increase from ~7.3 billion to over 9 billion people by 2050. Together with rising economic growth, this is forecast to result in a 50% increase in fuel demand, which will have to be met while reducing carbon dioxide (CO 2) emissions by 50–80% to maintain social, political, energy and climate security. This tension between rising fuel demand and the requirement for rapid global decarbonization highlights the need to fast‐track the coordinated development and deployment of efficient cost‐effective renewable technologies for the production of CO 2 neutral energy. Currently, only 20% of global energy is provided as electricity, while 80% is provided as fuel. Hydrogen (H2) is the most advanced CO 2‐free fuel and provides a ‘common’ energy currency as it can be produced via a range of renewable technologies, including photovoltaic (PV), wind, wave and biological systems such as microalgae, to power the next generation of H2 fuel cells. Microalgae production systems for carbon‐based fuel (oil and ethanol) are now at the demonstration scale. This review focuses on evaluating the potential of microalgal technologies for the commercial production of solar‐driven H2 from water. It summarizes key global technology drivers, the potential and theoretical limits of microalgal H2 production systems, emerging strategies to engineer next‐generation systems and how these fit into an evolving H2 economy.

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Section: Biological Challenges and Bottlenecks For Microalgal H2 Prodmentioning

confidence: 97%

Challenges and opportunities for hydrogen production from microalgae

Oey

Sawyer

Ross

et al. 2016

Plant Biotechnology Journal

145

View full text Add to dashboard Cite

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“…To enhance H 2 production similar strategies as for lipid synthesis have been undertaken with mixed results. The hydrogenase has been engineered to decrease its sensitivity with oxygen (132) and overexpressed to enhance production up to 10-fold (133). Another strategy is to interfere with the photosystem II.…”

Section: Hydrogen Gas Generationmentioning

confidence: 99%

Genetic manipulation of microalgae for the production of bioproducts

et al. 2018

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Microalgae have been used during the past four decades in the Bio-industries for the production of high added value products and development of useful approaches with environmental applications. The fast growing rate, simple growth requirements and using sunlight as the major source of energy are the key factors for usage of algae. In the past 15 years, a considerable progress has been made regarding the use of microalgae for production of proteins, nutraceuticals, food supplements, molecular tags for diagnostics and fixation of greenhouse gases. Nevertheless, genetic manipulation of microalgae still remains a fairly un-explored area which could boost the production of bioproducts. It is anticipated that in the near future use of microalgae will revolutionize its applications in diverse industries. The aim of this work is to present a critical review on potential of microalgae for the production of high-added value molecules, their practical applications, and the role of genetic engineering in its utilization as a unique niche in industry. In addition, current challenges within synthetic biology approaches are discussed.

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“…Alternatively, the endogenous hydrogenase has been overexpressed in Chlorella sp. strain DT, resulting in 7–10-fold hydrogen production under semi-aerobic conditions ( Chien et al, 2012 ).…”

Section: Enhancement Of Biohydrogen Evolutionmentioning

confidence: 99%

In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity

2015

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The great phylogenetic diversity of microalgae is corresponded by a wide arrange of interesting and useful metabolites. Nonetheless metabolic engineering in microalgae has been limited, since specific transformation tools must be developed for each species for either the nuclear or chloroplast genomes. Microalgae as production platforms for metabolites offer several advantages over plants and other microorganisms, like the ability of GMO containment and reduced costs in culture media, respectively. Currently, microalgae have proved particularly well suited for the commercial production of omega-3 fatty acids and carotenoids. Therefore most metabolic engineering strategies have been developed for these metabolites. Microalgal biofuels have also drawn great attention recently, resulting in efforts for improving the production of hydrogen and photosynthates, particularly triacylglycerides. Metabolic pathways of microalgae have also been manipulated in order to improve photosynthetic growth under specific conditions and for achieving trophic conversion. Although these pathways are not strictly related to secondary metabolites, the synthetic biology approaches could potentially be translated to this field and will also be discussed.

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Solar-to-bioH2 production enhanced by homologous overexpression of hydrogenase in green alga Chlorella sp. DT

Cited by 37 publications

References 41 publications

Challenges and opportunities for hydrogen production from microalgae

Challenges and opportunities for hydrogen production from microalgae

Genetic manipulation of microalgae for the production of bioproducts

In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity

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