Solarplast®—An Enzymatically Treated Spinach Extract

Simon, Annie; Mazhar, Shahneela; Хохлова, Е. В.; Leeuwendaal, Natasha; Phipps, Christopher; Deaton, John G.; Rea, Kieran; Colom, Joan

doi:10.3390/plants12142678

Cited by 4 publications

(2 citation statements)

References 43 publications

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“…Oxidants, such as reactive oxygen species (ROS) and reactive nitrogen species (RNS), can generate free radicals that can cause severe oxidative damage to cellular lipids, membranes, proteins, and DNA ( Mazhar et al, 2023 ; Simon et al, 2023b ). Antioxidants can scavenge these free radicals and prevent cellular oxidative stress by enzymatic and non-enzymatic mechanisms ( Valko et al, 2007 ).…”

Section: Resultsmentioning

confidence: 99%

In vitro safety and functional characterization of the novel Bacillus coagulans strain CGI314

Mazhar,

Simon,

Khokhlova

et al. 2024

Front. Microbiol.

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IntroductionBacillus coagulans species have garnered much interest in health-related functional food research owing to their desirable probiotic properties, including pathogen exclusion, antioxidant, antimicrobial, immunomodulatory and food fermentation capabilities coupled with their tolerance of extreme environments (pH, temperature, gastric and bile acid resistance) and stability due to their endosporulation ability.MethodsIn this study, the novel strain Bacillus coagulans CGI314 was assessed for safety, and functional probiotic attributes including resistance to heat, gastric acid and bile salts, the ability to adhere to intestinal cells, aggregation properties, the ability to suppress the growth of human pathogens, enzymatic profile, antioxidant capacity using biochemical and cell-based methods, cholesterol assimilation, anti-inflammatory activity, and attenuation of hydrogen peroxide (H2O2)-induced disruption of the intestinal-epithelial barrier.ResultsB. coagulans CGI314 spores display resistance to high temperatures (40°C, 70°C, and 90°C), and gastric and bile acids [pH 3.0 and bile salt (0.3%)], demonstrating its ability to survive and remain viable under gastrointestinal conditions. Spores and the vegetative form of this strain were able to adhere to a mucous-producing intestinal cell line, demonstrated moderate auto-aggregation properties, and could co-aggregate with potentially pathogenic bacteria. Vegetative cells attenuated LPS-induced pro-inflammatory cytokine gene expression in HT-29 intestinal cell lines and demonstrated broad antagonistic activity toward numerous urinary tract, intestinal, oral, and skin pathogens. Metabolomic profiling demonstrated its ability to synthesize several amino acids, vitamins and short-chain fatty acids from the breakdown of complex molecules or by de novo synthesis. Additionally, B. coagulans CGI314’s strong antioxidant capacity was demonstrated using enzyme-based methods and was further supported by its cytoprotective and antioxidant effects in HepG2 and HT-29 cell lines. Furthermore, B. coagulans CGI314 significantly increased the expression of tight junction proteins and partially ameliorated the detrimental effects of H2O2 induced intestinal-epithelial barrier integrity.DiscussionTaken together these beneficial functional properties provide strong evidence for B. coagulans CGI314 as a promising potential probiotic candidate in food products.

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Section: Resultsmentioning

confidence: 99%

In vitro safety and functional characterization of the novel Bacillus coagulans strain CGI314

Mazhar,

Simon,

Khokhlova

et al. 2024

Front. Microbiol.

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“…The treatment times for GSH and HP were determined by referring to previous research [ 21 , 41 , 42 ]. The optimal concentrations of each sample were determined by preliminary examination ( Supplementary Materials ).…”

Section: Methodsmentioning

confidence: 99%

Comprehensive Transcriptome Profiling of Antioxidant Activities by Glutathione in Human HepG2 Cells

Uchida,

Ferdousi,

Takahashi

et al. 2024

Molecules

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Glutathione (GSH) has long been recognised for its antioxidant and detoxifying effects on the liver. The hepatoprotective effect of GSH involves the activation of antioxidative systems such as NRF2; however, details of the mechanisms remain limited. A comparative analysis of the biological events regulated by GSH under physiological and oxidative stress conditions has also not been reported. In this study, DNA microarray analysis was performed with four experiment arms including Control, GSH, hydrogen peroxide (HP), and GSH + HP treatment groups. The GSH-treated group exhibited a significant upregulation of genes clustered in cell proliferation, growth, and differentiation, particularly those related to MAPK, when compared with the Control group. Additionally, liver functions such as alcohol and cholesterol metabolic processes were significantly upregulated. On the other hand, in the HP-induced oxidative stress condition, GSH (GSH + HP group) demonstrated a significant activation of cell proliferation, cell cycle, and various signalling pathways (including TGFβ, MAPK, PI3K/AKT, and HIF-1) in comparison to the HP group. Furthermore, several disease-related pathways, such as chemical carcinogenesis–reactive oxygen species and fibrosis, were significantly downregulated in the GSH + HP group compared to the HP group. Collectively, our study provides a comprehensive analysis of the effects of GSH under both physiological and oxidative stress conditions. Our study provides essential insights to direct the utilisation of GSH as a supplement in the management of conditions associated with oxidative stress.

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Research on Topic Mining and Evolution Trends of Functional Agriculture Based on the BERTopic Model

Lin,

Xin,

Peng

et al. 2024

Agriculture

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Based on the BERTopic model, this study analyzes 15,744 scientific papers in the field of functional agriculture from 1995 to 2024 to uncover core themes and evolutionary trends in global functional agriculture, and particularly focuses on revealing the developmental trajectory in China. The results indicate that global functional agriculture research is characterized by diverse themes and intensive study, forming a multi-topic cross-network centered on plant chemical extraction and agricultural soil research, with a focus on food nutrition, human health, and environmental protection. By contrast, China’s functional agriculture research demonstrates a more focused and in-depth approach, concentrating on functional food development and agricultural environmental protection themes, with notable growth trends in areas such as selenium-enriched products and resistant starch. Combined with China’s agricultural development environment, this study makes the following suggestions for the development of functional agriculture in China: (1) Promoting interdisciplinary cooperation between functional agriculture and other technologies. (2) Developing agricultural products with Chinese characteristics and forming Chinese functional agricultural product brands. (3) Utilizing smart farming technology to boost functional agriculture.

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Solarplast®—An Enzymatically Treated Spinach Extract

Cited by 4 publications

References 43 publications

In vitro safety and functional characterization of the novel Bacillus coagulans strain CGI314

In vitro safety and functional characterization of the novel Bacillus coagulans strain CGI314

Comprehensive Transcriptome Profiling of Antioxidant Activities by Glutathione in Human HepG2 Cells

Research on Topic Mining and Evolution Trends of Functional Agriculture Based on the BERTopic Model

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