Solid intraperitoneal Landschütz tumour in mice: implications in cancer chemotherapy and immunology studies

Abstract

Multiple intraperitoneal injections of various normal sera into BALB/c mice inoculated intraperitoneally with Landschütz ascites tumour cells abrogated the development of ascitic syndrome in almost all the animals. In a large proportion of the survivors solid intraperitoneal tumours developed, composed of characteristic ascites tumour cells engulfed and encapsulated in connective tissue. The effect of serum on the development of the solid tumour was diminished if the donor had been immunized against mouse IgG. Inoculated animals treated with serum hyperimmune against mouse IgG showed accelerated ascitic tumour growth. Cyclophosphamide or arabinosylcytosine strongly inhibited growth of solid tumours. Simultaneous administration of arabinosylcytosine and its antagonist cycloheximide did not interrupt tumour growth.

Differences in production of solid Landhscütz tumours in BALB/c and ICR mice

“…They were confined in the BALB{c mice to the peritoneal wall and the internal layer of the abdominal muscle and did not penetrate into the subcutaneous tissue but did so in the ICR mice causing extensive ulceration of the skin. The histological appearance of the tumours has already been described (Stuart & EI Hassan, 1964;Hod et al, 1975). During regression there was massive infiltration of mononuclear cells through the abdominal muscles and tumour tissue could hardly be recognized owing to watery and fibroid degeneration.…”

“…The Landschiitz tumour was discovered, and has mostly been studied, in the ascites form. But solid tumours can be produced if tumour cells are inoculated subcutaneously or if they are given in-traperitoneaJly and the mice are treated with an immune serum from rabbits (EI Hassan & Stuart, 1966) or with normal sera or even saline (Hod, Zimber, Gidoni & Schonfeld, 1975). The tumour is apparently transplantable in all mouse strains and even in rats but there are differences in host resistance.…”