Solid−Liquid Extraction Laser Excited Time-Resolved Shpol'skii Spectrometry:  A Facile Method for the Direct Detection of 15 Priority Pollutants in Water Samples

Bystol, Adam J.; Whitcomb, Jennifer L.; Campiglia, Andrés D.

doi:10.1021/es010575b

Cited by 30 publications

(32 citation statements)

References 10 publications

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“…Attractive features include a simple experimental procedure and compatibility with portable instrumentation. Due to the non-destructive nature of luminescence measurements, extraction membranes can be brought to the lab for subsequent sample elution and fluorophore confirmation via high-resolution techniques [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Alfarhani

Al-Tameemi

Schenone

et al. 2016

Microchemical Journal

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a b s t r a c tBenzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B[a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-related cancer. This article focuses on the development of screening methodology for the routine analysis of B[a]P metabolites in urine samples. It explores the solid-surface room-temperature fluorescence (RTF) properties of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-trans-9,10-dihydrodiol, benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol and benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples with octadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6% (3-hydroxy-benzo[a]pyrene) are obtained with the aid of fiber optic probe that eliminates the need for manual optimization of signal intensities. Analytical recoveries from human urine samples varied from 87.5 ± 3.1% (3-hydroxy-benzo[a]pyrene) to 99.8 ± 2.5% (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figures of merit and the simplicity of the experimental procedure demonstrate the potential of this approach for screening biomarkers of PAH exposure in numerous urine samples.

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Section: Introductionmentioning

confidence: 99%

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Alfarhani

Al-Tameemi

Schenone

et al. 2016

Microchemical Journal

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“…Recently, Campiglia and coworkers (149)(150)(151)(152)(153)(154)(155)(156) resurrected the use of Shpol'skii spectrometry for the determination of PAHs. Whereas none of the early studies of Shpol'skii spectrometry focused on the EPA 16 PAHs, most of the efforts of Campiglia and coworkers (149)(150)(151)(152)(153)(154)(155)(156) were directed towards this list of PAHs.…”

Section: Shpol'skii Spectrometrymentioning

confidence: 99%

“…Whereas none of the early studies of Shpol'skii spectrometry focused on the EPA 16 PAHs, most of the efforts of Campiglia and coworkers (149)(150)(151)(152)(153)(154)(155)(156) were directed towards this list of PAHs. In the early 2000s they reported improved methodology for laser-excited time-resolved Shpol'skii spectrometry (LETRSS) of PAHs at 77 K (liquid nitrogen) and 4.2 K (liquid helium) based on cryogenic fiber optic probes in the sample, excitation with frequency doubled tunable dye laser, and detection using an intensified coupled device (155).…”

Section: Shpol'skii Spectrometrymentioning

confidence: 99%

Analytical Methods for Determination of Polycyclic Aromatic Hydrocarbons (PAHs) — A Historical Perspective on the 16 U.S. EPA Priority Pollutant PAHs

Wise

Sander

Schantz

2015

Polycyclic Aromatic Compounds

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The identification of 16 polycyclic aromatic hydrocarbons (PAHs) as priority pollutants by the U.S. Environmental Protection Agency (EPA) in 1976 has been a primary driver for analytical methods development for the determination of PAHs. In this article, the historical development of methods in liquid chromatography (LC) and gas chromatography (GC) to separate these 16 PAHs is discussed. In LC a significant effort was the search for and the fundamental understanding of the unique stationary phase capable of achieving the desired separation of the 16 EPA PAHs. For GC methods, the focus on stationary phase development has been the separation of critical isomers with a broader scope than the 16 EPA PAHs. The current routine LC and GC methods for the 16 EPA PAHs are well established; however, new advances in analytical techniques beyond LC and GC are discussed. Many analysts are now interested in more than just the 16 EPA PAHs (e.g., higher molecular mass PAHs and alkyl-substituted PAHs) and analytical methods have emerged to address these needs. Reference materials and their use in the determination of PAHs are discussed.

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“…In cases where the exact sample composition was unknown and the formulation of a correct blank for lifetime background subtraction was impossible, the fluorescence decay at the base of the target peak was used for background subtraction at the target wavelength. The accuracy of this procedure was investigated previously (18). Origin software (version 5, Microcal Software, Inc.) was used for curve fitting of fluorescence lifetimes.…”

Section: K Letrss Analysis Of Hplc Fractionsmentioning

confidence: 99%

“…In a later paper, SLE was coupled to 77 K LETRSS, and 15 EPA PAH were determined in a spiked Red River sample without previous separation (18). The analysis of more complex samples was not attempted.…”

Section: Introductionmentioning

confidence: 99%

Laser-Induced Multidimensional Fluorescence Spectroscopy in Shpol'skii Matrixes for the Analysis of Polycyclic Aromatic Hydrocarbons in HPLC Fractions and Complex Environmental Extracts

Bystol

Thorstenson

Campiglia

2002

Environ. Sci. Technol.

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Fluorescence measurements are easily made with the aid of a cryogenic fiber optic probe. Emission wavelength time matrixes, excitation emission matrixes, and time-resolved excitation emission matrixes are rapidly collected with a pulsed tunable dye laser, a spectrograph, and an intensified charged-coupled device. Compound identification is based on spectral and lifetime information. The potential of this approach for supporting high-performance liquid chromatography analysis and for the direct determination of benzo[a]pyrene without previous separation is demonstrated.

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Solid−Liquid Extraction Laser Excited Time-Resolved Shpol'skii Spectrometry: A Facile Method for the Direct Detection of 15 Priority Pollutants in Water Samples

Cited by 30 publications

References 10 publications

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Analytical Methods for Determination of Polycyclic Aromatic Hydrocarbons (PAHs) — A Historical Perspective on the 16 U.S. EPA Priority Pollutant PAHs

Laser-Induced Multidimensional Fluorescence Spectroscopy in Shpol'skii Matrixes for the Analysis of Polycyclic Aromatic Hydrocarbons in HPLC Fractions and Complex Environmental Extracts

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