Solid-phase extraction and optimized separation of doxorubicin, epirubicin and their metabolites using reversed-phase high-performance liquid chromatography

Nicholls, G. D.; Clark, Brian J.; Brown, John E.

doi:10.1016/0731-7085(91)80104-h

Cited by 37 publications

(22 citation statements)

References 13 publications

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“…Besides silica based reversedphase C18 [46,47,56,60,66,67,70], C8 [72] and C2 [44,65] sorbents, also polymeric Oasis HLB [48,49,57,71] and MCX [50] sorbents have been employed. A Biotrap 500 MS online SPE column has also been used [69].…”

Section: Solid Phase Extractionmentioning

confidence: 99%

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Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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Section: Solid Phase Extractionmentioning

confidence: 99%

“…One method could be applied to each of the four pairs (main compound and respective reduced metabolite) individually [68]. Four methods were developed that could determine simultaneously two or more main compounds alone [69] or together with their reduced metabolites [13,[70][71][72].…”

Section: Analytes and Concentrations Of Interestmentioning

confidence: 99%

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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“…At evenly distributed times between 0 and 2 h, a fixed volume of the culture was removed, and cells pelleted by centrifugation. Free doxorubicin in the supernatant was extracted and measured by a sensitive and specific high-performance liquid chromatography technique [20]. Binding was calculated by comparison with cell-free doxorubicin solutions incubated in parallel.…”

Section: Binding Modelmentioning

confidence: 99%

Mathematical and computational models of drug transport in tumours

Groh

Hubbard

Jones

et al. 2014

J. R. Soc. Interface.

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The ability to predict how far a drug will penetrate into the tumour microenvironment within its pharmacokinetic (PK) lifespan would provide valuable information about therapeutic response. As the PK profile is directly related to the route and schedule of drug administration, an in silico tool that can predict the drug administration schedule that results in optimal drug delivery to tumours would streamline clinical trial design. This paper investigates the application of mathematical and computational modelling techniques to help improve our understanding of the fundamental mechanisms underlying drug delivery, and compares the performance of a simple model with more complex approaches. Three models of drug transport are developed, all based on the same drug binding model and parametrized by bespoke in vitro experiments. Their predictions, compared for a 'tumour cord' geometry, are qualitatively and quantitatively similar. We assess the effect of varying the PK profile of the supplied drug, and the binding affinity of the drug to tumour cells, on the concentration of drug reaching cells and the accumulated exposure of cells to drug at arbitrary distances from a supplying blood vessel. This is a contribution towards developing a useful drug transport modelling tool for informing strategies for the treatment of tumour cells which are 'pharmacokinetically resistant' to chemotherapeutic strategies.

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“…Compared to RPLC, the normal phase retention behavior of HILIC offers the potential for dramatic changes in selectivity with quite simple mobile phase. The compositions of the eluents used in most RPLC methods are comparatively complex [15][16][17][18][19][20], and phosphate buffers as well as anionic surfactants are favored in HPLC-UV but not in MS detection. It can be expected that HILIC on silica with high-organic low-ionic-strength volatile buffer will be a good choice for developing LC-MS assay for quantifications of epirubicin and its metabolites in biological matrices.…”

Section: Effect Of Sample Sizementioning

confidence: 99%

“…1. Several RPLC methods have been developed for separation of epirubicin and related compounds [15][16][17][18][19][20]. In our previous work, milligram amounts of epirubicin were isolated from raw product by RPLC carried out in the overloaded elution mode [21] and in the displacement mode [22] on an analytical-scale Kromasil KR100-10C 18 column.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic behavior of epirubicin and its analogues on high-purity silica in hydrophilic interaction chromatography

2004

Journal of Chromatography A

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Hydrophilic interaction chromatography has been applied for the separation of epirubicin and its analogues using high-purity silica column with aqueous-organic mobile phase. Parameters affecting the chromatographic behavior of the solutes such as organic modifier, buffer pH, ionic strength and sample size, have been investigated. Of utmost importance for successful separation of these analogues is the choice of organic modifier, since it impacts both the solvent selectivity and the ionization of silica silanols as well as buffer solution, and consequently the retention behavior of solutes. Acetonitrile was shown to offer superior separation of these analogues to methanol, isopropanol or tetrahydrofuran. Results of the effects of organic modifier, buffer pH and ion strength indicate that the retention mechanism is a mixed-mode of adsorption and ion exchange. In addition, an irreversible adsorption of these compounds was found on silica in the weakly acidic or neutral mobile phases, and the effect of various factors on irreversible adsorption was also preliminarily discussed. More significantly, these basic compounds have exhibited peaks with a slanted front and a sharp tail, a typical overloading peak profile belonging to the behavior of competitive anti-Langmuir isotherm by increasing the sample size at the experimental conditions.

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Solid-phase extraction and optimized separation of doxorubicin, epirubicin and their metabolites using reversed-phase high-performance liquid chromatography

Cited by 37 publications

References 13 publications

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Mathematical and computational models of drug transport in tumours

Chromatographic behavior of epirubicin and its analogues on high-purity silica in hydrophilic interaction chromatography

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