Solid-phase extraction–liquid chromatographic method for the determination and pharmacokinetic studies of albiflorin and paeoniflorin in rat serum after oral administration of Si–Wu decoction

Sheng, Yuxin; Li, Lie; Wang, Chuanshe; Li, Yanyan; Guo, Dan

doi:10.1016/j.jchromb.2004.03.041

Cited by 25 publications

(19 citation statements)

References 24 publications

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“…[2][3][4][5][6][7]14) To our excitement, swertisin, which was deglucosed from spinosin and/or 6ٞ-feruloylspinosin by hepatic metabolism or enteral germs, was detected in all kinds of bio-samples and in particular with a larger amount in feces for the first report. This compound, reaching the systemic circulation, is therefore correlated to drug bioactivities [2][3][4][5][6][7]14) and suggests that TCMs have characteristics of transformation in vivo. The extremital Fig.…”

Section: Metabolic Study Of Flavonoid Extract Of Jujube Seeds In Rat mentioning

confidence: 99%

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Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn)

Bao

et al. 2009

CHEMICAL & PHARMACEUTICAL BULLETIN

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Semen Ziziphi Spinosae (jujube seeds), the dried semen of Ziziphus jujuba MILL. var. spinosa (BUNGE) HU ex H. F. CHOU, is one of the most famous traditional Chinese medicines (TCMs) with hypnosis, sedation, and anticonvulsivant effects.1) It has been widely used for the treatment of insomnia in clinical practice.2) Chemical investigations on jujube seeds resulted in the discovery of several groups of bioactive components including flavonoids, saponins, and fatty acids. 3,4) Modern pharmacological studies have demonstrated that flavonoids are the main bioactive compounds responsible for the sedative and hypnotic effects of jujube seeds. 2,5) Hence it is important to explicate the biotransformation of flavonoids in vivo so as to clarify the mechanism of pharmacological action and to promote its availability as well. To date, there are few reports regarding the physiological disposition of flavonoids after oral administration of jujube seeds-containing TCMs 6,7) ; however, the biotransformation of flavonoids in vivo remains unknown. The challenges for the metabolic study in vivo of TCMs extract lie in two aspects: one is the complexity of TCMs containing hundreds of constituents, and the other is the lack of proper analytical methods for the identification of the metabolites even in trace amount. 8)A major goal of this study was to develop a highly specific and sensitive high-performance liquid chromatography method coupled with diode-array detection and ion-trap mass spectrometry (HPLC-DAD-MS n ) for the study of the constituents and metabolites in rat plasma, urine, and feces after oral administration of flavonoid extract of jujube seeds, providing scientific plausibility for the activity of this extract. These achievements would support preclinical pharmacokinetic studies and achieve a better understanding of the pharmacological action mechanism of flavonoid extract of jujube seeds. ExperimentalMaterials Jujube seeds were purchased from a genuine area Xintai (Hebei, China) and identified by one of the authors, Dr. Ping Li. Spinosin (with a purity of 99.0%), and 6ٞ-feruloylspinosin (with a purity of 99.0%) (Fig. 1) was prepared in our laboratory. Male Sprague-Dawley (SD) rats were acquired from the Laboratory Animal Center of China Pharmaceutical University (Nanjing, China). HPLC grade acetonitrile was purchased from Merck KGaA Company (Merck, Germany). Deionized water was purified by Milli-Q system (Millipore, U.S.A.). Physiological saline was purchased from Shijiazhuang Pharmaceutical Corporation (Shijiazhuang, China). Heparin sodium, obtained from Hanbang Science & Technology (Nanjing, China), was dissolved in physiological saline at a concentration of 1250 U/ml and used to rinse the test tubes prior to blood collection for plasma. Glacial acetic acid, as well as the other reagents, was of analytical grade.Preparation of Flavonoid Extract from Jujube Seeds Jujube seeds (3.0 kg) were ground into powder (particle size 20-40 mesh) and refluxed with 24 l of petroleum ether (60-90°C) three times (each for 12 h). Af...

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Section: Metabolic Study Of Flavonoid Extract Of Jujube Seeds In Rat mentioning

confidence: 99%

“…The results are quite consistent with previous publications in which flavonoids are reported to be extensively degraded by the intestinal microflora or enzymes in vivo. [6][7][8][9][10][11][17][18][19] …”

Section: Metabolic Study Of Flavonoid Extract Of Jujube Seeds In Rat mentioning

confidence: 99%

Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn)

Bao

et al. 2009

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…It has been widely used for thousands of years for the treatment of women's diseases, such as anemia, relief of menstrual discomfort, climacteric syndrome, and dysmenorrhea. Recent study has reported that it also possesses sedative, anticoagulant, antibacterial activities and exhibits vasodilatation, enhancement of cellular immunity, and radio-protection effects [1,2].…”

Section: Introductionmentioning

confidence: 99%

Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS

Zhu

Duan

Tang

et al. 2014

Acta Chromatographica

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Summary. An efficient and robust method for analyzing constituents of a well-known traditional Chinese medicine (TCM) formula SiWu decoction (SWD) contains Angelicae Sinensis Radix (ASR), Chuanxiong Rhizoma (CR), Paeoniae Radix Alba (PRA), and Rehmanniae Radix Praeparata (RRP) by ultra high-performance liquid chromatography (UHPLC)/time-of-flight mass spectrometry (TOF-MS) was established. The method efficiently applied to the separation of 75 compounds, including organic acids, phthalides, phenylpropanoid glycosides, iridoid glycosides, monoterpene glycosides, and galloyl glycosides in the complex prescription, 52 compounds in this study can be unambiguously identified or tentatively characterized. The separation was achieved within 20 min at the optimized chromatographic conditions. Our study provided a reliable and high-efficient method for the understanding of the chemical basis of SWD.

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“…Cell-and animal-based studies have shown that paeoniflorin and other monoterpene glycosides exhibit anti-inflammatory, antiplatelet, antithrombosis, antioxidative, and neuroprotective properties [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] . Due to these pharmacological properties, several bioanalytical assays were developed to measure plasma concentrations of paeoniflorin and albiflorin [34][35][36] . However, PK data regarding the monoterpene glycosides remain quite limited, particularly human PK data.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and disposition of monoterpene glycosides derived from Paeonia lactiflora roots (Chishao) after intravenous dosing of antiseptic XueBiJing injection in human subjects and rats

Cheng

Lin

et al. 2016

Acta Pharmacol Sin

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Aim: Monoterpene glycosides derived from Paeonia lactiflora roots (Chishao) are believed to be pharmacologically important for the antiseptic herbal injection XueBiJing. This study was designed to characterize the pharmacokinetics and disposition of monoterpene glycosides. Methods: Systemic exposure to Chishao monoterpene glycosides was assessed in human subjects receiving an intravenous infusion and multiple infusions of XueBiJing injection, followed by assessment of the pharmacokinetics of the major circulating compounds. Supportive rat studies were also performed. Membrane permeability and plasma-protein binding were assessed in vitro. Results: A total of 18 monoterpene glycosides were detected in XueBiJing injection (content levels, 0.001-2.47 mmol/L), and paeoniflorin accounted for 85.5% of the total dose of monoterpene glycosides detected. In human subjects, unchanged paeoniflorin exhibited considerable levels of systemic exposure with elimination half-lives of 1.2-1.3 h; no significant metabolite was detected. Oxypaeoniflorin and albiflorin exhibited low exposure levels, and the remaining minor monoterpene glycosides were negligible or undetected. Glomerular-filtration-based renal excretion was the major elimination pathway of paeoniflorin, which was poorly bound to plasma protein. In rats, the systemic exposure level of paeoniflorin increased proportionally as the dose was increased. Rat lung, heart, and liver exposure levels of paeoniflorin were lower than the plasma level, with the exception of the kidney level, which was 4.3-fold greater than the plasma level; brain penetration was limited by the poor membrane permeability. Conclusion: Due to its significant systemic exposure and appropriate pharmacokinetic profile, as well as previously reported antiseptic properties, paeoniflorin is a promising XueBiJing constituent of therapeutic importance.

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Solid-phase extraction–liquid chromatographic method for the determination and pharmacokinetic studies of albiflorin and paeoniflorin in rat serum after oral administration of Si–Wu decoction

Cited by 25 publications

References 24 publications

Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn)

Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn)

Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS

Pharmacokinetics and disposition of monoterpene glycosides derived from Paeonia lactiflora roots (Chishao) after intravenous dosing of antiseptic XueBiJing injection in human subjects and rats

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