Solid-phase microextraction-comprehensive two-dimensional gas chromatography-time of flight-mass spectrometry (SPME-GC×GC-TOF-MS) of non-steroidal anti-inflammatory drugs from water

Abstract

Solid-phase microextraction with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (SPME-GCxGC-ToFMS) was used for the qualitative analysis of nine non-steroidal anti-inflammatory drugs (NSAIDs) from water. This work focused on the selectivity of the extraction and on the added selectivity provided by the addition of the second column in the chromatography for the analysis of underivatized NSAIDs. Selectivity due to fiber selection, solution pH and extraction temperature is discussed. The final extraction conditions obtained were polydimethylsiloxane/carboxen/divinylbenzene fiber, pH 3.2, temperature 70 o C and extraction time 30 minutes. The extracted NSAIDs were then analyzed by GCxGC-ToFMS and the chromatographic selectivity using a combination of a 5% phenyl polydimethyl siloxane column in the first dimension and a trifluoropropyl polysiloxane column in the second dimension is discussed. This method can be applied to determine the NSAIDs in complex matrices such as urine, blood for clinical toxicology for trace level analysis.

Tavşan Plazmasında Naproksenin GC-MS Yöntemi ile Analizi

“…In addition, it is seen that the GC-MS method developed and validated by us has a better mountain range in plasma than previous studies (Vittal et al, 2019;Hamid and Elsaman, 2017;Pushpa and Sailaja, 2020;Aresta et al, 2005;Shimek et al, 1982;Navalon et al, 1999;Westerlund et al, 1979;Hsu et al, 2006;Sultan et al, 2005;Goktas et al, 2020;Hlozek et al, 2014;Muthal and Snow, 2016).…”

“…In literature research, high-performance liquid chromatography (HPLC) (Vittal et al, 2019;Hamid and Elsaman, 2017;Pushpa and Sailaja, 2020;Satterwhite and Boudinot, 1988;Upton et al;1980;Vree et al, 1992;Aresta et al, 2005;Hirai et al, 1997;Shimek et al, 1982;Navalon et al, 1999;Westerlund et al, 1979;Hsu et al, 2006;Mikami et al, 2000), liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Sultan et al, 2005;Pietruk et al, 2021) and gas chromatography-mass spectrometry (GC-MS) (Krokos et al, 2018;Goktas et al, 2020;Hlozek et al, 2014;Muthal and Snow, 2016) methods for determining naproxen in biological materials have been reached.…”

“…There are different chromatographic studies on the determination of naproxen in biological materials. Among these, the most used methods are usually HPLC (Vittal et al, 2019;Hamid and Elsaman, 2017;Pushpa and Sailaja, 2020;Satterwhite and Boudinot, 1988;Upton et al;1980;Vree et al, 1992;Aresta et al, 2005;Hirai et al, 1997;Shimek et al, 1982;Navalon et al, 1999;Westerlund et al, 1979;Hsu et al, 2006;Mikami et al, 2000), LC-MS/MS (Sultan et al, 2005;Pietruk et al, 2021) and GC-MS (Krokos et al, 2018;Goktas et al, 2020;Hlozek et al, 2014;Muthal and Snow, 2016).…”

“…In the literature, naproxen was extracted with a solid-phase extraction method from human plasma in previous studies (Aresta et al, 2005;Hirai et al, 1997;Mikami et al, 2000;Muthal and Snow, 2016). The solid-phase extraction technique is an expensive method.…”