Solid phase microextraction for quantitative analysis – Expectations beyond design?

Nolvachai, Yada; Amaral, Michelle S.S.; Herron, Riley; Marriott, Philip J.

doi:10.1016/j.greeac.2022.100048

Cited by 23 publications

(3 citation statements)

References 129 publications

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“…Statistical analyses for peaks that met these criteria were performed using R Studio (version 1.3.1093). Quantitation of absolute amounts of compounds in SPME samples can be problematic ( Nolvachai et al 2023 ), so quantitative analyses of each peak were performed on the area of that peak expressed as a percentage of the total areas of all peaks in the sample. The Best Normalize package ( Peterson 2021 ) was used to identify transformations, almost always OrderNorm ( Peterson and Cavanaugh 2020 ), that met the assumptions of normality and heteroscedasticity required in the following analyses.…”

Section: Methodsmentioning

confidence: 99%

Differential pheromone profile as a contributor to premating isolation between two sympatric sibling fruit fly species

Castro-Vargas,

Oakeshott,

Yeap

et al. 2024

Journal of Insect Science

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Bactrocera tryoni (Froggatt) and Bactrocera neohumeralis (Hardy) are sibling fruit fly species that are sympatric over much of their ranges. Premating isolation of these close relatives is thought to be maintained in part by allochrony—mating activity in B. tryoni peaks at dusk, whereas in B. neohumeralis, it peaks earlier in the day. To ascertain whether differences in pheromone composition may also contribute to premating isolation between them, this study used solid-phase microextraction and gas chromatography-mass spectrometry to characterize the rectal gland volatiles of a recently collected and a more domesticated strain of each species. These glands are typical production sites and reservoirs of pheromones in bactrocerans. A total of 120 peaks were detected and 50 were identified. Differences were found in the composition of the rectal gland emissions between the sexes, species, and recently collected versus domesticated strains of each species. The compositional variation included several presence/absence and many quantitative differences. Species and strain differences in males included several relatively small alcohols, esters, and aliphatic amides. Species and strain differences in females also included some of the amides but additionally involved many fatty acid esters and 3 spiroacetals. While the strain differences indicate there is also heritable variation in rectal gland emissions within each species, the species differences imply that compositional differences in pheromones emitted from rectal glands could contribute to the premating isolation between B. tryoni and B. neohumeralis. The changes during domestication could also have significant implications for the efficacy of Sterile Insect Technique control programs.

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Section: Methodsmentioning

confidence: 99%

Differential pheromone profile as a contributor to premating isolation between two sympatric sibling fruit fly species

Castro-Vargas,

Oakeshott,

Yeap

et al. 2024

Journal of Insect Science

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“…This green technique offers several advantages, such as simplicity, low cost, drastic solvent reduction, and improved accuracy, so it was successfully used in various analytical applications. The original design of the device involved a syringe equipped with a fiber coated with a suitable sorbent phase, and several improvements have been made to this technique [ 19 , 20 , 21 , 22 , 23 , 24 ]. In addition, SPME provides two different extraction methods: the direct immersion mode (DI-SPME), where the fiber is fully submersed in the aqueous phase, and the headspace mode (HS-SPME), by placing the fiber in the space above the sample [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Development of In-Needle SPME Devices for Microextraction Applied to the Quantification of Pesticides in Agricultural Water

Alshehri,

Hammami,

Alshehri

et al. 2024

Molecules

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The chemical industry explosion in the 20th century has led to increased environmental pollution, affecting fauna, flora, and waterways. These substances alter water’s taste, color, and smell, making it unfit for consumption or toxic. Agricultural water networks face threats from pollution before and after treatment. Some chemical contaminants, like pesticides, are embedded in natural biogeochemical cycles. In this study, we developed a simple and low-cost procedure for the fabrication of needles coated with polydimethylsiloxane (PDMS) as an efficient sorbent for the microextraction of organic pollutant traces from water. The prepared needles were used as an alternative for commercial solid-phase micro-extraction (SPME) devices in analytical chemistry. The PDMS polymeric phase was characterized by Fourier-transform infrared spectroscopy (FT-IR), thermogravimetry (TGA), and scanning electron microscopy (SEM). The PDMS-coated needles were used for extraction of thirteen pesticides by direct-immersion solid-phase microextraction (DI-SPME) from contaminated waters, followed by determination with gas chromatography-mass spectrometry (GC-MS). The developed analytical method showed limits of detection (LODs) between 0.3 and 2.5 ng mL−1 and RSDs in the range of 0.8–12.2%. The homemade needles were applied for the extraction of pesticides in surface and ground aqueous samples collected from an agricultural area. Several target pesticides were identified and quantified in the investigated water samples.

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“…Sun et al [26] reviewed research associated with different sample preparation methods, such as steam distillation [17,27], simultaneous distillation extraction [25], dynamic [18] and static [19] headspace, direct thermal desorption [21], direct microwave desorption [28], liquid-liquid extraction (LLE) [29], stir bar sorptive extraction (SBSE) [23], and headspace solid-phase microextraction (HS-SPME) applied to hop [22,30]. This last method attracts attention as being a "greener" approach since it is a solventless technique using sorptive polymeric fibres, is simple, versatile, and has a wide applications base, with SPME reported for samples in the environmental industry, food, drugs, and other areas, although the quantitative application of SPME is rather complex [22,31].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Two-Dimensional Gas Chromatography–Mass Spectrometry as a Tool for the Untargeted Study of Hop and Their Metabolites

Resende,

Amaral,

Botelho

et al. 2024

Metabolites

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Since hop secondary metabolites have a direct correlation with the quality of beer and other hop-based beverages, and the volatile fraction of hop has a complex composition, requiring effective separation, here we explore the application of headspace solid-phase microextraction as a sample preparation method, coupled with comprehensive two-dimensional gas chromatography–mass spectrometry (GC×GC–MS) analysis. The methodology involved the use of a DVB/PDMS fibre with 500 mg of hop cone powder, extracted for 40 min at 50 °C, for both GC–MS and GC×GC–MS. The varieties Azacca, Cascade, Enigma, Loral, and Zappa were studied comprehensively. The results demonstrate that GC×GC–MS increases the number of peaks by over 300% compared to classical GC–MS. Overall, 137 compounds were identified or tentatively identified and categorised into 10 classes, representing between 87.6% and 96.9% of the total peak area. The composition revealed the highest concentration of sesquiterpene hydrocarbons for Enigma, whilst Zappa showed a relatively significant concentration of monoterpene hydrocarbons. Principal component analysis for all compounds and classes, along with hierarchical cluster analysis, indicated similarities between Zappa and Cascade, and Azacca and Loral. In conclusion, this method presents an optimistic advancement in hop metabolite studies with a simple and established sample preparation procedure in combination with an effective separation technique.

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Solid phase microextraction for quantitative analysis – Expectations beyond design?

Cited by 23 publications

References 129 publications

Differential pheromone profile as a contributor to premating isolation between two sympatric sibling fruit fly species

Differential pheromone profile as a contributor to premating isolation between two sympatric sibling fruit fly species

Development of In-Needle SPME Devices for Microextraction Applied to the Quantification of Pesticides in Agricultural Water

Comprehensive Two-Dimensional Gas Chromatography–Mass Spectrometry as a Tool for the Untargeted Study of Hop and Their Metabolites

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