Solid-phase microextraction integrated nanobiosensors for the serial detection of cytoplasmic dopamine in a single living cell

Chang, Yaran; Yongjia, Chen; Shao, Yunlong; Li, Boye; Wu, Yuanyuan; Zhang, Wenmei; Zhou, Yang; Yu, Zhihui; Lu, Liping; Wang, Xiayan; Guo, Guangsheng

doi:10.1016/j.bios.2020.112915

Cited by 25 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accurate measurements of cytoplasmic DA in intact pre- or postsynaptic neurons have been challenging due to lack of sensitivity of most analytical methods and their effects on cell viability ( Chang et al, 2021 ; Olefirowicz and Ewing, 1990 ; Post and Sulzer, 2021 ). However, given that DA is present at high millimolar concentrations within the synaptic vesicles ( Omiatek et al, 2013 ; Zhang et al, 2020 ), it is likely that rapid uptake of DA post release will result in high cytoplasmic DA concentrations.…”

Section: Discussionmentioning

confidence: 99%

The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus

Puri

Romano

Lin

et al. 2022

eLife

View full text Add to dashboard Cite

Dopamine is a key catecholamine in the brain and the kidney, where it is involved in a number of physiological functions such as locomotion, cognition, emotion, endocrine regulation and renal function. As a membrane impermeant hormone and neurotransmitter, dopamine is thought to signal by binding and activating dopamine receptors, members of the G protein couple receptor (GPCR) family, only on the plasma membrane. Here, using novel nanobody-based biosensors, we demonstrate for the first time that the dopamine D1 receptor (D1DR), the primary mediator of dopaminergic signaling in the brain and kidney, not only functions on the plasma membrane but becomes activated at the Golgi apparatus in the presence of its ligand. We present evidence that activation of the Golgi pool of D1DR is dependent on Organic Cation Transporter 2 (OCT2), a dopamine transporter, providing an explanation for how the membrane impermeant dopamine accesses subcellular pools of D1DR. We further demonstrate that dopamine activates Golgi-D1DR in murine striatal medium spiny neurons (MSN) and this activity depends on OCT2 function. We also introduce a new approach to selectively interrogate compartmentalized D1DR signaling by inhibiting Gas coupling, using a nanobody-based chemical recruitment system. Using this strategy, we show that Golgi-localized D1DRs regulate cAMP production and mediate local protein kinase A activation. Together, our data suggest that spatially compartmentalized signaling hubs are previously unappreciated regulatory aspects of D1DR signaling. Our data provide further evidence for the role of transporters in regulating subcellular GPCR activity.

show abstract

Section: Discussionmentioning

confidence: 99%

The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus

Puri

Romano

Lin

et al. 2022

eLife

View full text Add to dashboard Cite

show abstract

“…Most often—for instance, in the works cited above—the instruments used for this purpose are mass spectrometers [ 25 – 27 ], although other options have also been explored. For instance, in their recent work, Chang et al integrated polypyrrole modified carbon fibers with a working electrode for the extraction of dopamine from single living cells followed by electrochemical detection [ 28 ]. In this work, the authors demonstrated that their developed nanoprobe, which had a conical tip with a diameter of less than 100 nm and a coating thickness of ca.…”

Section: Miniaturization Of Spme Devicesmentioning

confidence: 99%

“…C The schematic of electrochemical detection using an after extracted bifunctional nanoprobe as a WE. Reprinted from [ 28 ] with permission …”

Section: Miniaturization Of Spme Devicesmentioning

confidence: 99%

“…Nonetheless, the most common SPME-MS couplings are not the only options for directly interfacing SPME with analytical instrumentation. For instance, as discussed above, Chang et al coupled SPME with electrochemical detection [ 28 ]. Furthermore, recent publications have detailed the coupling of SPME with a fluorimeter for the screening of doxorubicin [ 32 ].…”

Section: Rapid and Sensitive Determination Of Target Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Solid-phase microextraction: a fit-for-purpose technique in biomedical analysis

Bojko

2022

Anal Bioanal Chem

View full text Add to dashboard Cite

Solid-phase microextraction (SPME) possesses unique features that allow it to be used in analyses that would not be possible with traditional sample-preparation methods. The simplicity of SPME protocols and extraction devices makes it a uniform platform for analyzing biological samples, either via the headspace or in direct immersion mode. Furthermore, flexible probe design enables SPME to be applied to target objects of different sizes, offering analysis on a scale ranging “from single cell to living organs”. SPME microfibers are minimally invasive, which enables them to be applied for the spatial and temporal monitoring of target analytes or to assess changes in the entire metabolome or lipidome. Furthermore, SPME permits the capture of the elusive portion of the metabolome, thus complementing exhaustive methods that are biased towards highly abundant and stable species. Significantly, SPME can be interfaced with analytical instrumentation to create a rapid diagnostic tool. However, despite these advantages, SPME has some limitations that must be well-understood and addressed. This paper presents examples of up-to-date applications of SPME, challenges related to particular studies, and future perspectives regarding the application of SPME in biomedical analysis.

show abstract

“…Abnormal levels of dopamine in body fluids can cause various neurological diseases, such as schizophrenia or Parkinson’s disease [ 1 ]. Moreover, it has profound significance to control DA at a level suitable for the normal activities of human cells [ 2 ]. Therefore, accurate and convenient detection of DA is an important and valuable work in the field of biology.…”

Section: Introductionmentioning

confidence: 99%

Peroxidase-Like Platinum Clusters Synthesized by Ganoderma lucidum Polysaccharide for Sensitively Colorimetric Detection of Dopamine

et al. 2021

View full text Add to dashboard Cite

The sensitive and selective detection of dopamine (DA) is very important for the early diagnosis of DA-related diseases. In this study, we reported the colorimetric detection of DA using Ganoderma lucidum polysaccharide (GLP) stabilized platinum nanoclusters (Ptn-GLP NCs). When Pt600-GLP NCs was added, 3,3’,5,5’-tetramethylbenzidine (TMB) was rapidly catalyzed and oxidized to blue oxTMB, indicating the peroxidase-like activity of Pt600-GLP NCs. The catalytic reaction on the substrate TMB followed the Michaelis-Menton kinetics with the ping-pong mechanism. The mechanism of the colorimetric reaction was mainly due to the formation of hydroxyl radical (•OH). Furthermore, the catalytic reaction of Pt600-GLP NCs was used in the colorimetric detection of DA. The linear range for DA was 1–100 μM and the detection limit was 0.66 μM. The sensitive detection of DA using Pt-GLP NCs with peroxidase-like activity offers a simple and practical method that may have great potential applications in the biotechnology field.

show abstract

Solid-phase microextraction integrated nanobiosensors for the serial detection of cytoplasmic dopamine in a single living cell

Cited by 25 publications

References 30 publications

The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus

The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus

Solid-phase microextraction: a fit-for-purpose technique in biomedical analysis

Peroxidase-Like Platinum Clusters Synthesized by Ganoderma lucidum Polysaccharide for Sensitively Colorimetric Detection of Dopamine

Contact Info

Product

Resources

About