Solid-phase microextraction-liquid chromatography-mass spectrometry applied to the analysis of insecticides in honey

Abstract

. Solid-phase microextraction-liquid chromatographymass spectrometry applied to the analysis of insecticides in honey. Food Additives and Contaminants, 2007, 25 (01) AbstractAn approach based on solid-phase microextraction-liquid chromatography-mass spectrometry (SPME-LC-MS) has been developed for determining 12 insecticides (bromophos ethyl, chlorpyrifos methyl, chlorpyrifos ethyl, diazinon, fenoxycarb, fonofos, phenthoate, phosalone, pirimiphos methyl, profenofos, pyrazophos, and temephos) in honey. The influence of several parameters on the efficiency of the SPME was systematically investigated. Under optimal conditions, the procedure provided excellent linearity (>0.990), detection and quantification limits (between 0.001 and 0.1 µg g -1 and between 0.005 and 0.5 µg g -1 , respectively), and precision (<19 % at the quantification limits and from 6 to 14 % at ten times higher concentrations). However, recoveries were not so good, ranging from 19 to 92 %. Honey samples were found which were contaminated with bromophos ethyl, diazinon, fonofos, pirimiphos ethyl, pyrazophos and temephos at estimated concentrations from 6.2 ± 1.2 to 19 ± 3 ng g -1 .

The occurrence of pesticides and persistent organic pollutants in Italian organic honeys from different productive areas in relation to potential environmental pollution

“…Mevinphos was found in honey from both Trentino Alto Adige and Calabria, with a prevalence of 67% and 86%, respectively. The samples from Lombardia showed the fewest number of OPs; the highest prevalence (37%) was for Captan, a fungicide that is mainly used for diseases of apples during the growing season (Berrie & Cross, 2005;Blasco et al, 2003;Blasco et al, 2008), with a maximum concentration of 20.56 This result is surprising considering that the use of allopathic chemically synthesised medicinal products for preventive bee treatments is prohibited for organic system productions. Several other studies have previously demonstrated that the chemicals used by beekeepers inside the hives are frequently found in the apicultural matrices (Pedersen et al, 2006;Lambert et al, 2013, Garry et al, 2016.…”

Headspace single-drop microextraction of common pesticide contaminants in honey–method development and comparison with other extraction methods

“…The analysis of pesticide contaminants in honey demands extremely sensitive and selective analytical methods to meet the trace levels of pesticides occurrence in honey and overcome the analytical problems derive from complex matrix and HS-SDME coupled with gas chromatography and a selective detector could be a successive alternative approach for rapid screening purposes. For this purpose several common pesticide contaminants (diazinon, lindane, chlorpyrifos ethyl, p,p 0 -DDE, and endosulfan) that may occur in honey [5,[18][19][20][21][22] (Table 1) were selected to be analysed by gas chromatography and electron capture detection using HS-SDME, D-SDME [10] and traditional liquid-liquid extraction (LLE) [20]. The applicability of HS-SDME was studied by the use of a multivariate optimization and estimated through the comparison of the obtained analytical performance of the optimized method with D-SDME and LLE.…”

“…Solid-phase microextraction (SPME) [3][4][5][6][7], stir bar sorptive extraction (SBSE) [8,9], single-drop microextraction (SDME) [10], and dispersive liquid phase microextraction (DLPME) [11] are some of these recently developed techniques the goal to which is to reduce overall analysis time and solvent consumption, without comprising the analytical results. The achievements in the field of multiresidue analysis in complex food matrices by the different microextraction techniques have been well reported in several reviewing papers [12][13][14][15] and their applicability in the field of trace analysis in complex matrices is still in demand since the success of a multi-residue analysis in dietary products still relies on the appropriate choice of sample preparation and clean-up methodologies.…”

Quality of Meliponinae honey: Pesticides residues, pollen identity, and microbiological profiles

“…The monitoring of pesticide residues is essential to evaluate the potential risk to consumer health and also to provide relevant information on the use of chemicals in proximity to beehive cultivation. Analytical methodologies for pesticides identification and quantification are recent, and the majority of the published reports refers to the honey of A. mellifera (Blasco, Font, & Picó, 2007;Orso et al, 2014Orso et al, , 2016Tette et al, 2016). There are various methods to detect pesticides in foods, and the QuEChERS method, whose acronym means Quick, Easy, Cheap, Effective, Rugged, and Safe, is one of the most suitable among them (Anastassiades, Lehotay, Stajnbaher, & Schenck, 2003;Prestes, Friggi, Adaime, & Zanella, 2009).…”