Solid‐Phase Radioimmunoassay of Serum IgG, IgM, and IgA Antibodies to Cytomegalovirus

Torfason, Einar G.; Källander, C. F. R.; Halonen, Pekka

doi:10.1002/jmv.1890070202

Cited by 20 publications

(10 citation statements)

References 21 publications

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“…Higher CMV-specific antibody titres reflect recent or reactivated CMV infection (Musiani et al, 1988) and there is evidence that they are correlated with the CMV antigenemia test which is a quantitative marker for CMV load (van Zanten et al, 1995). In addition the increase in the titre of CMV-specific IgG that is observed in association with ageing is believed the reflect the increased frequency of viral reactivation within this cohort (Stowe et al, 2007;Torfason et al, 1981). The implication of our findings is that it is the magnitude of the CMVspecific immune response, rather than CMV infection itself, that is associated with symptoms of depression and anxiety.…”

Section: Discussionmentioning

confidence: 53%

Cytomegalovirus is associated with depression and anxiety in older adults

Phillips

Carroll

Khan

et al. 2008

Brain, Behavior, and Immunity

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Infection with cytomegalovirus (CMV), a beta-herpesvirus, is common within the population.Although asymptomatic, infection is associated with increased serum concentrations of cytokines such as TNFα and IL-6, which are also related to mood and wellbeing. The present study examined whether infection with CMV was associated with mood in a community-based sample of older adults. Blood samples and scores on the General Health Questionnaire were available for 137 participants. Serum was analysed for the presence of CMV-specific IgG and the antibody titre was used as an indirect measure of viral load. The majority of the participants (66%) were CMV seropositive and seropositive status was not associated with psychological morbidity. However, within the CMV positive group, individuals with higher CMV-specific antibody titres were more likely to be depressed, anxious, and suffer more overall psychological morbidity. This association could be mediated by the impact of affect-moderating cytokines secreted through the CMV-specific immune response.

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Section: Discussionmentioning

confidence: 53%

Cytomegalovirus is associated with depression and anxiety in older adults

Phillips

Carroll

Khan

et al. 2008

Brain, Behavior, and Immunity

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“…The detection of a higher prevalence of specific IgA to HSV and VZV in APFP patients shown in this study adds further support to the postulated role of these viruses in the etiology of APFP. Virus-specific IgA has been detected in both primary and recurrent VZV and CMV infection [Brunnel et al, 1975; Sarov, 1980, 1981;Sarov et al, 1981;Torfason et al, 1981;Haikin and Sarov, 1982;Sarov et al, 19821. HSV-2 IgA antibody to membrane antigen has been found to be detected significantly more frequently and at higher titers in patients with cervical dysplasia than among matched controls [Mendis et al, 19811.…”

Section: Discussionmentioning

confidence: 99%

Specific IgG and IgA antibodies to herpes simplex virus and varicella zoster virus in acute peripheral facial palsy patients

Hadar

Tovi

Sidi

et al. 1983

Journal of Medical Virology

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The role of herpes simplex virus (HSV) and varicella zoster virus (VZV) in acute peripheral facial palsy (APFP) was evaluated in 153 patients. The sera of patients were examined for IgG and IgA antibodies to HSV and VZV by the immunoperoxidase antibody-membrane antigen (IPAMA) technique. The prevalence of IgG and IgA seropositivity to HSV was significantly higher in the APFP patients than in the matched control group. No significant difference was found in the geometric mean titer (GMT) to HSV antibodies in the APFP patients compared to the matched control groups. The prevalence of IgA antibodies to VZV was significantly higher in the APFP patients group than in the matched control group. The GMT for VZV IgG antibodies was significantly higher in the APFP patients than in the matched control group. No significant difference was found in the GMT of VZV IgA antibodies. Eleven of 83 patients for whom paired sera were available had significantly increased or decreased IgG and IgA antibody titers to VZV on subsequent examination. Four of these patients did not show any evidence of zosterian eruption. These studies support the concept that VZV and HSV might have a role in the etiopathogenesis of APFP in some of the patients.

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“…Overall, 78% of the 32 women who delivered infected infants and 65% of the 17 who delivered uninfected babies possessed IgM antibodies. Or, stated from the point of view of serological results, of the 36 women that possessed IgM antibodies, only 25 (69%) transmitted the infection in utero. Of the 13 women that had undetectable IgM antibodies, 7 (54%) delivered infected infants.…”

Section: Ndmentioning

confidence: 95%

Immunoglobulin M antibodies detected by enzyme-linked immunosorbent assay and radioimmunoassay in the diagnosis of cytomegalovirus infections in pregnant women and newborn infants

Stagno¹,

Tinker²,

Elrod³

et al. 1985

J Clin Microbiol

132

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Immunoglobulin M (IgM) antibodies were detected by a commercially available enzyme-Linked Immunosorbent Assay (ELISA) in 36 of 49 (73%) pregnant women with primary cytomegalovirus (CMV) infection. A positive ELISA-IgM result occurred in 10 of 13 patients (77%) assessed within 8 weeks of seroconversion. The sensitivity of the radioimmunoassay (RIA) to identify primary infection in pregnant women was comparable, 78% in general and 86% for women tested within 16 weeks of seroconversion. Of the 36 women with primary infection who had detectable IgM antibodies by ELISA, 25 (69%) were delivered of congenitally infected infants, whereas of the 13 with undetectable IgM antibodies, 7 (54%) transmitted the infection in utero. IgM antibodies were detected by ELISA in only 5 of 43 (11%) women who experienced a recurrence of CMV which either did or did not result in congenital infection. RIA was less likely to measure CMV-specific IgM in recurrent infection, inasmuch as 1 of 19 (5.2%) women with proven recurrent infection had detectable IgM antibody, giving RIA a better specificity for primary infection. Specific IgM antibodies were detected by ELISA in 42 of 61 (69%) babies congenitally infected with CMV and in 4 of 70 (5.7%) uninfected control newborn infants. The RIA was superior for diagnosis of congenital CMV infection, with a sensitivity of 89% and a specificity of 100%. The lower sensitivity of the ELISA-IgM occurred in the category of congenitally infected infants born to mothers with recurrent infection (43%), a group that is at the lowest risk of disease or to develop sequalae. This commercially available ELISA-IgM could be used in combination with a CMV-specific IgG test for monitoring women during pregnancy for primary infection.

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Solid‐Phase Radioimmunoassay of Serum IgG, IgM, and IgA Antibodies to Cytomegalovirus

Cited by 20 publications

References 21 publications

Cytomegalovirus is associated with depression and anxiety in older adults

Cytomegalovirus is associated with depression and anxiety in older adults

Specific IgG and IgA antibodies to herpes simplex virus and varicella zoster virus in acute peripheral facial palsy patients

Immunoglobulin M antibodies detected by enzyme-linked immunosorbent assay and radioimmunoassay in the diagnosis of cytomegalovirus infections in pregnant women and newborn infants

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