Solid sampling technique for direct detection of condensed tannins in bark by matrix‐assisted laser desorption/ionization mass spectrometry

Abstract

A novel method for the direct analysis of condensed tannin components in bark was developed on the basis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a simple solid sampling technique. The MALDI mass spectra obtained from the wood (bark) powder sample clearly showed a series of peaks corresponding to the sodium ion adducts of condensed tannin oligomers up to around m/z 3000. The results indicate that the condensed tannins in the bark sample used in this work mostly consist of combinations of flavan-3-ol units such as profisetinidin (PF), prorobinetinidin (PR) and prodelphinidin (PD), at least up to 10-mers (m/z approximately 3000).

Direct profiling and imaging of plant metabolites in intact tissues by using colloidal graphite‐assisted laser desorption ionization mass spectrometry

“…Interestingly, intense signals from ion species at m/z 717 were observed at the stigma. This species has not been unambiguously identified, but it has spectral similarity to that of condensed tannin compounds (Behrens et al, 2003;Ishida et al, 2005) based on its fragment ions species (at m/z 602, 339, 311, 309, 291, and 247).…”

“…However, the applicability of LDI MS to imaging plant metabolites, including direct profiling of cuticular wax compounds (Sluszny et al, 2005), analysis of phosphatidylcholine (Zabrouskov et al, 2001), condensed tannins in bark (Ishida et al, 2005), and profiling of plant carotenoids (Fraser et al, 2007), has been successfully demonstrated. The advantage of LDI MS, over the 'hyphenated techniques' (GC-, LC-, and capillary electrophoresis (CE)-MS) is the ability to sample biochemicals directly without any extraction protocol.…”

Tannins from Canarium album with potent antioxidant activity

“…Individual oligomers of vegetable tannins are well resolved in MALDI-TOF MS spectra with their molecular weights determinated in the analysis of condensed tannins (Ohnishi-Kameyama et al, 1997;Yang and Chien, 2000;Ishida et al, 2005). When MALDI-TOF MS is used to characterize tannins, the (Krueger et al, 2003).…”

Preliminary investigation of the central nervous system effects of ‘Tira‐capeta’ (Removing the Devil), a cigarette used by some Quilombolas living in Pantanal Wetlands of Brazil

“…Phytochemical studies with Syzygium aromaticum ('cravo-da-Índia') showed the following constituents: essential oils (Juliani et al, 2006), flavonoids (Nassar, 2006), gallic acid (Pathak et al, 2004), phenylpropanoids (Miyazawa and Hisama, 2003) and tannins (Tanaka et al, 1996). In the Eucalyptus globulus (eucalyptus) were found essential oils (Cimanga et al, 2002); triterpenic acids and fatty acids (Pereira et al, 2005;Guo and Yang, 2006), phloroglucinols (Singh and Bharate, 2006), flavonoids (Ikawati et al, 2001) and condensed tannins (Ishida et al, 2005). Many of the chemical constituents present in Allium sativum (garlic) are known (Amagase, 2006).…”