Solid-state culture of Aspergillus niger on support

Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct

“…Apparently, sugar cane bagasse acted as only a solid inert support and the microbial growth and enzyme production were stimulated by wheat bran and orange bagasse. The use of sugar cane bagasse as inert support was suitable for the growth and pectinase production by Aspergillus niger, P. viridicatum RFC3 and Thermoascus aurantiacus (Oriol et al, 1988;Silva et al, 2002;Martins et al, 2002). The quantities of PG and PL obtained in this study were high compared to those reported for pectinolytic strains such as Aspergillus niger (25 U Pg and 350 U Pl per gram of substrate), P. italicum (6U Pl/g e 25U Pg/g), P. frequentans (3.4 U Pg /g) and A. foedidus (1000 U Pl/g) cultivated on solid substrates (Garzón and Hours, 1992;Hours et al, 1994;Castilho et al, 2000).…”

General and microbiological aspects of solid substrate fermentation

“…However, the moisture level at which free moisture becomes apparent varies considerably between substrates and is dependant upon their water binding characteristics. For example, free water is observed when the moisture content exceeds 40% in maple bark and 50-55% in rice and cassava (Oriol et al, 1988a). With most lignocellulosic substrates free water becomes apparent before the 80% moisture level is reached (Moo-Young et al, 1983).…”

“…SSF is a batch process using natural heterogeneous materials (Raimbault, 1981 andTengerdy, 1985), containing complex polymers like lignin (Agosin et al, 1989), pectin (Kumar, 1987;Oriol, 1988a), lignocellulose (Roussos, 1985). SSF has been focused mainly to the production of feed, hydrolytic enzymes, organic acids, gibberelins, flavours and biopesticides.…”

On-line monitoring of citric acid production in solid-state culture by respirometry

“…Sugarcane bagasse, with 0.8-2.0 mm particle diameter range and exhaustively washed with distilled water, was used as a support [4,8] absorbing the nutrient medium at a ratio of 17:67 and sterilised by steaming for 1 h. Inoculum, prepared as described above, was added and mixed using aseptic conditions. The resulting initial moisture content was 73%.…”

“…Automatic on-line analysis of CO 2 and O 2 in the exit gases coming from SSC reactors allows real-time information on the physiological state of the cultures to be obtained and correlation with other parameters such as biomass [2][3][4] to be established.…”