2009
DOI: 10.1002/mrc.2444
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Solid‐state NMR spectroscopic studies on the interaction of sorbic acid with phospholipid membranes at different pH levels

Abstract: 2H, 31P, and 1H-magic-angle-spinning (MAS) solid-state NMR spectroscopic methods were used to elucidate the interaction between sorbic acid, a widely used weak acid food preservative, and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bilayers under both acidic and neutral pH conditions. The linewidth broadening observed in the 31P NMR powder pattern spectra and the changes in the 31P longitudinal relaxation time (T1) indicate interaction with the phospholipid headgroup upon titration of sorbic acid or dec… Show more

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Cited by 16 publications
(14 citation statements)
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“…Future experiments should establish to what extent these and other preservatives have uncoupling properties and what the quantitative effect of ⌬ depletion on the growth rate is. It is likely that their ability to cross the membrane barrier (log K ow ) into the aqueous phase will play a role, as seems to be the case with sorbic acid and acetic acid (42,43).…”
Section: Discussionmentioning
confidence: 99%
“…Future experiments should establish to what extent these and other preservatives have uncoupling properties and what the quantitative effect of ⌬ depletion on the growth rate is. It is likely that their ability to cross the membrane barrier (log K ow ) into the aqueous phase will play a role, as seems to be the case with sorbic acid and acetic acid (42,43).…”
Section: Discussionmentioning
confidence: 99%
“…2 H quadrupolar splittings of 2 H labels on the entire acyl chain of the POPC-d 31 lipids reflect dynamics of the lipid bilayer hydrophobic region [34, 35, 42], S CD order parameters calculated from the 2 H quadrupolar splittings yield individual 2 HH dynamics along the acyl chain. 2 H nuclei near the membrane surface have larger order parameters than the ones near the membrane core since 2 H nuclei near the membrane surface are more restricted [41].…”
Section: Discussionmentioning
confidence: 99%
“…Membrane structure and dynamic changes induced by the incorporation of proteins into the phospholipid bilayer can be revealed using 31 P solid-state NMR spectroscopy [33-35]. Also, 2 H solid-state NMR spectroscopy of 2 H labels along the lipid acyl chain can be used to study the dynamics of the hydrophobic region of the membrane [34-42], In this study, interactions between lipid bilayers and WT-PLB, P-PLB and R9C-PLB are investigated from the membrane perspective using 31 P and 2 H solid-state NMR spectroscopy. These experiments shed light onto the loss of functionality of the R9C mutation from the membrane perspective.…”
Section: Introductionmentioning
confidence: 99%
“…Finally, recent NMR data show that sorbic acid interacts mostly with head groups of membrane phospholipids rather than inserting deeply into the membrane [34 ]. This observation suggests that the compound does not have a detergent-like mode of action, but rather interferes with the membrane surface thus possibly modulating the activity of membrane proteins, that is amino-acid permeases similar to the situation observed in yeast [35] and Aspergillus niger [36].…”
Section: Weak Organic Acid Resistance and Adaptation In Bacillimentioning
confidence: 86%